Electrospray ionization mass spectrometric method for the determination of cannabinoid precursors:N-acylethanolamine phospholipids (NAPEs)

Hansen, Henrik H.; Hansen, Steen Honoré; Bjørnsdottir, Inga; Hansen, Harald S.

doi:10.1002/(sici)1096-9888(199907)34:7<761::aid-jms832>3.0.co;2-r

Cited by 29 publications

(27 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). Based on tandem MS (MS2 and MS3), we speculated that this cluster of ions could potentially be N-acylated phosphatidylethanolamines, although it should be noted that unequivocal assignment can only be ascertained by more careful comparisons with the fragmentation patterns of N-acylated phosphatidylethanolamine standards (Guan et al, 2006;Hansen et al, 1999).…”

Section: Targeted and Non-targeted Analysis Of Membrane Lipids Using supporting

confidence: 87%

“…(D) Identification of ions of interest by tandem mass spectrometry, in this case, m/z 1028. The product ion spectrum is consistent with previously published work using synthetic N-acylated phosphatidylethanolamine (NAPE) (Hansen et al, 1999). Inset shows proposed structures of NAPE species with m/z 1028 (18:0 NPE (16:0/22:6) and 16:0 NPE (18:0/22:6)).…”

Section: Targeted Lipid Analysis Using Tandem Mass Spectrometrymentioning

confidence: 92%

See 1 more Smart Citation

Targeted and Non-Targeted Analysis of Membrane Lipids Using Mass Spectrometry

Guan

Wenk

2012

Methods in Cell Biology

View full text Add to dashboard Cite

Section: Targeted and Non-targeted Analysis Of Membrane Lipids Using supporting

confidence: 87%

Section: Targeted Lipid Analysis Using Tandem Mass Spectrometrymentioning

confidence: 92%

Targeted and Non-Targeted Analysis of Membrane Lipids Using Mass Spectrometry

Guan

Wenk

2012

Methods in Cell Biology

View full text Add to dashboard Cite

“…A method to characterize synthetic NAPE molecular species by direct infusion into the mass detector was reported previously ( 39 ). However, although this analytical approach may help to study the ionization processes and fragmentation patterns of synthetic NAPEs, it is not suitable for the analyses of NAPEs in a complex biological matrix such as brain tissue.…”

Section: Lc Separation Of Napesmentioning

confidence: 99%

Identification of biosynthetic precursors for the endocannabinoid anandamide in the rat brain

Astarita

Ahmed

Piomelli

2008

Journal of Lipid Research

View full text Add to dashboard Cite

“…NAPEs have been previously directly measured by two methods: TLC [26] and mass spectrometry [2; 26; 27; 28; 29]. While TLC is readily available and inexpensive, it lacks specificity for individual N -acyl chains and is less sensitive and more difficult to accurately quantify than mass spectrometry methods.…”

Section: Introductionmentioning

confidence: 99%

A liquid chromatography–tandem mass spectrometry method for measurement of N-modified phosphatidylethanolamines

Guo

Amarnath

Davies

2010

Analytical Biochemistry

View full text Add to dashboard Cite

N-acyl phosphatidylethanolamines (NAPEs) are synthesized in response to stress in a variety of organisms from bacteria to man. More recently, non-enzymatic modification of the ethanolamine headgroup of PE by various aldehydes including levuglandin/isoketals, which are γ-ketoaldehydes (γKAs) derived from arachidonic acid, has also been demonstrated. The levels of these various N-modified PEs formed during stress and their biological significance remain to be fully characterized. Such studies require an accurate, facile, and cost-effective method for quantifying N-modified PEs. Previously, NAPE and some of the non-enzymatically N-modified PE species have been quantified by mass spectrometry after hydrolysis to their constituent N-acylethanolamine by enzymatic hydrolysis, most typically with S. chromofuscus phospholipase D (PLD). However, enzymatic hydrolysis is not cost-effective for routine analysis of large number of samples and hydrolytic efficiency may vary for different N-modified PEs, making quantitation more difficult. We therefore sought a robust and inexpensive chemical hydrolysis approach. Methylamine (CH3NH2) mediated deacylation has previously been used in headgroup analysis of phosphatidylinositol phosphates. We therefore developed an accurate assay for NAPEs and γKA-PEs using CH3NH2 mediated deacylation and quantitation of the resulting glycerophospho-N-modified ethanolamines by LC/MS/MS.

show abstract

Electrospray ionization mass spectrometric method for the determination of cannabinoid precursors:N-acylethanolamine phospholipids (NAPEs)

Cited by 29 publications

References 42 publications

Targeted and Non-Targeted Analysis of Membrane Lipids Using Mass Spectrometry

Targeted and Non-Targeted Analysis of Membrane Lipids Using Mass Spectrometry

Identification of biosynthetic precursors for the endocannabinoid anandamide in the rat brain

A liquid chromatography–tandem mass spectrometry method for measurement of N-modified phosphatidylethanolamines

Contact Info

Product

Resources

About