Electrospray ionization mass spectrometry fingerprinting of propolis

Sawaya, Alexandra Christine Helena Frankland; Tomazela, Daniela M.; Cunha, Ildenize B. S.; Bankova, Vassya; Marcucci, María Cristina; Custodio, Angela R.; Eberlin, Marcos N.

doi:10.1039/b403873h

Cited by 133 publications

(131 citation statements)

References 42 publications

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“…This difference may be related to the varying species of Populus encountered by the bees; to differences in climate and soil which lead to variations in the biochemical pathways of plant secondary metabolites or to differences in the seasons when the resins were collected. This result is similar to variations observed in a previous study [19] in the composition of green propolis in Brazil.…”

Section: Resultssupporting

confidence: 92%

“…Both EASI and ESI mass spectra indicate similar chemical profiles with only variations in ion abundances. On the whole, the spectra obtained by EASI-MS were similar to those obtained by ESI-MS in previous studies [19]. Fig.…”

Section: Chemometric Analysis Of Datasupporting

confidence: 86%

“…The major components of the Korean samples placed in this group (K39-Geochang, K41-Muju and K38-Sangju) were identified by Ahn et al [8] using HPLC. These flavonoids are also quite common in propolis from the South of Brazil and Central Chile [6] and their ions have been observed in ESI-MS of samples from the USA and Europe [19]. Group 1,2 comprises samples containing marker ions from both group 1 and group 2 with similar intensities.…”

Section: Resultsmentioning

confidence: 96%

“…A total of 42 samples of propolis ethanol extracts were analyzed and are listed in Table 1. Some of the samples were crude propolis and were extracted in ethanol, using the method described in Sawaya et al [19]. In brief, 600 mg of ground crude propolis were macerated in 2 mL of pure ethanol for 1 week at a temperature of 30 • C, and then filtered to remove the insoluble portion and the wax.…”

Section: Reagents and Samplesmentioning

confidence: 99%

“…Therefore, fast MS fingerprinting methods can be successfully applied to the high-throughput direct analysis of crude propolis samples, especially to determine sample origin. We performed the first systematic study applying ESI-MS fingerprinting to propolis [19], analyzing over 40 propolis samples, mainly from Brazil. Since then, ESI-MS fingerprinting has been applied to a wide variety of food and beverage samples [20][21][22] as well as natural products [23].…”

Section: Introductionmentioning

confidence: 99%

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Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

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a b s t r a c tChemical profiles of a representative set of 49 propolis ethanolic extracts collected worldwide (North and South America, Europe, Asia and Oceania) were obtained via easy ambient sonic-spray ionization mass spectrometry (EASI-MS). This simple and easily implemented fingerprinting technique analyses directly (without any pre-separation or sample manipulation) a tiny droplet of the ethanolic extract placed on a inert surface under ambient conditions. Data acquisition took about a minute per sample with no substantial sample carry-over. Extraction of propolis with ethanol by using an ultrasonic bath method gave EASI-MS data similar to the traditional maceration method, reducing total analysis time for the crude propolis resin from a week to just ca 1 h. Principal component analysis of the EASI-MS data is shown to group samples according to the plant sources of their resins, which characterizes their geographical origin.

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Section: Resultssupporting

confidence: 92%

Section: Chemometric Analysis Of Datasupporting

confidence: 86%

Section: Resultsmentioning

confidence: 96%

Section: Reagents and Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

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How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp‐2 Cells

Sforcin

Farlow

Crevelin

et al. 2023

Chemistry & Biodiversity

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The aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp-2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography-mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.

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Chemical Composition and Evaluation of Antibacterial, Antibiofilm, and Mutagenic Potentials of a Propolis Sample from the Atlantic Forest of Midwest Brazil

da Silva Mirowski,

da Silva Coutinho de Araújo Bueno,

Elsner Rodrigues

et al. 2023

Chemistry & Biodiversity

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Sixteen triterpenoids with various skeletal types, five phenylpropanoid derivatives, and two flavonoids were isolated from a propolis sample produced by Apis mellifera collected in the Atlantic Forest of Midwest Brazil. Among these compounds, six triterpenes, namely 3β,20R‐dihydroxylanost‐24‐en‐3‐yl‐palmitate, (23E)‐25‐methoxycycloartan‐23‐en‐3‐one, 24‐methylenecycloartenone, epi‐lupeol, epi‐a‐amyrin, and epi‐β‐amyrin are being reported for the first time in propolis, while cycloartenone, (E)‐cinnamyl benzoate, and (E)‐cinnamyl cinnamate are new findings in Brazilian propolis. The presence of cycloartane‐ and lanostane‐type triterpenoids, the latter being a class of compounds of restricted distribution in propolis worldwide, has not been reported in propolis from Midwest Brazil until now. The ethyl acetate phase obtained from the ethanol extract was effective in preventing biofilm formation by Staphylococcus aureus, with an inhibition rate of about 96% at 0.5 mg.mL‐1, and with quercetin isolated as one of its active constituents. In contrast, the hexane phase exhibited notable antibacterial activity against Pseudomonas aeruginosa, inhibiting bacterial growth by 92% at 0.5 mg.mL‐1; however, none of the triterpenoids isolated from this phase proved active against this pathogen. The ethanol extract was neither toxic nor mutagenic at the concentrations tested, as determined by the in vivo SMART assay on Drosophila melanogaster, even under conditions of high metabolic activation.

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Electrospray ionization mass spectrometry fingerprinting of propolis

Cited by 133 publications

References 42 publications

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp‐2 Cells

Chemical Composition and Evaluation of Antibacterial, Antibiofilm, and Mutagenic Potentials of a Propolis Sample from the Atlantic Forest of Midwest Brazil

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