Electrospun 3D Fibrous Scaffolds for Chronic Wound Repair

Chen, Huizhi; Peng, Yan; Wu, Shucheng; Tan, Lay Poh

doi:10.3390/ma9040272

Cited by 81 publications

(70 citation statements)

References 44 publications

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“…Fibrous scaffolds, which closely mimic natural ECM fibrous structure, have attracted great attention as replacement of the missing or dysfunctional ECM in skin regeneration . Particularly, electrospinning is an easy and versatile approach to the development of fibrous platforms in diverse designs, which is applied to fabricate fibrous substrates in this work.…”

Section: Resultsmentioning

confidence: 99%

Migration and Phenotype Control of Human Dermal Fibroblasts by Electrospun Fibrous Substrates

Chen

Lui

Tan

et al. 2019

Adv Healthcare Materials

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Electrospun fibrous matrices, mimicking extracellular matrix (ECM) hierarchical structures, are potential scaffolds for wound healing. To design functional scaffolds, it is important to explore the interactions between scaffold topographic features and cellular responses, especially directional migration and phenotypic changes, which are critical functional aspects during wound healing. Here, accelerated and persistent migration of human dermal fibroblasts (HDFs) is observed on fibers with aligned orientation. Furthermore, aligned fibers can induce fibroblast‐to‐myofibroblast differentiation of HDFs. During wound healing, the presence of myofibroblasts advances wound repair by rendering contractile force and ECM deposition within the early and middle courses, but its continuous persistence in the later event may not be desired due to the contribution in pathological scarring. To tune the balance, it is noted in this work that the introduction of matricellular protein angiopoietin‐like 4 (ANGPTL4) is capable of reversing the phenotypic alteration induced by aligned fibers, in a time‐dependent manner. These results indicate fibrous matrices with oriented configuration are functional in mediating directional cell migration and phenotypic change. The discoveries further suggest that tissue‐engineered fibrous grafts with precise alignment modulation and ANGPTL4 releasing properties may thus be promising to promote wound repair with minimizing scar formation.

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Section: Resultsmentioning

confidence: 99%

Migration and Phenotype Control of Human Dermal Fibroblasts by Electrospun Fibrous Substrates

Chen

Lui

Tan

et al. 2019

Adv Healthcare Materials

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“…Three general processes for the production of nanofibers at the laboratory scale include self-assembly, phase separation, and electrospinning process. Among these processes, electrospinning is the most common and industrial process for the production of nanofibers (Chen, Peng, Wu, & Tan, 2016;Khil, Cha, Kim, Kim, & Bhattarai, 2003;Kossovich, Salkovskiy, & Kirillova, 2010;Rho et al, 2006 (Heo, Yang et al, 2012).…”

Section: Nanofibersmentioning

confidence: 99%

“…Enhance HDFs infiltration; promote cellular proliferation; accelerate the regeneration rate of skin tissue and chronic wound closure regeneration rate of skin tissue and chronic wound (chen, Peng et al, 2016).…”

Section: Chronic Woundmentioning

confidence: 99%

Current status and future outlook of nano‐based systems for burn wound management

et al. 2019

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Wound healing process is a natural and intricate response of the body to its injuries and includes a well-orchestrated sequence of biochemical and cellular phenomena to restore the integrity of skin and injured tissues. Complex nature and associated complications of burn wounds lead to an incomplete and prolonged recovery of these types of wounds. Among different materials and systems which have been used in treating the wounds, nanotechnology driven therapeutic systems showed a great opportunity to improvement and enhancement of the healing process of different type of wounds. The aim of this study is to provide an overview of the recent studies about the various nanotechnology-based management of burn wounds and the future outlook of these systems in this area. Laboratory and animal models for assessing the efficacy of these systems in burn wound management also discussed. K E Y W O R D S burn wound, laboratory and animal models, multicomposites, nanomedicine, nanotechnologybased systems, nanoparticles, scaffolds

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“…Jinglei Wu et al 11 have reviewed various techniques to improve cell infiltration for electrospun scaffolds. Huizhi Chen et al 12 used a modified collector for improving cell infiltration by collecting fibers on water. Those techniques influence the bulk property of the electrospun mat.…”

Section: Fabrication and Characterization Of Electrospun Matsmentioning

confidence: 99%

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

Kumar¹

2017

ATROA

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Tissue engineered skin substitutes are used in chronic, partial or full thickness wounds, and serve to augment the growth and repair of the skin at the wound site, by acting as a temporary or permanent skin replacement. In this study Polycaprolactone a biodegradable polymer was electrospun in a binary solvent system to form nonwoven mats with porous fibers (PPCL). These mats were hydrolysed with alkali to make them more hydrophilic and promote cell adhesion. The electrospun mat served as a scaffold for culture of human keratinocyte (HaCaT) and rabbit hair follicle dermal fibroblast cells (HFDF) cells. F-actin staining and SEM analysis showed that the cells exhibited their characteristic morphology, with good adhesion and spreading. Live dead staining confirmed the viability of the cells on the PPCL mat. A bioengineered construct of HaCaT and HFDF cells cultured on either side of the PPCL mat was generated in vitro. Light microscopy of Toluidine blue stained cryo sections of the construct reveal that the HaCaT cells formed intact multilayers, while the fibroblasts showed a more distributed growth pattern with migration into the mat, hence emulating the epidermis and dermis of the skin. The results were substantiated in the SEM micrographs and fluorescent images of DiI and DiO labeled cocultures. The PPCL mat cocultured with HaCaT and HFDF cells thus finds potential as a self organized tissue engineered skin substitute..

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Electrospun 3D Fibrous Scaffolds for Chronic Wound Repair

Cited by 81 publications

References 44 publications

Migration and Phenotype Control of Human Dermal Fibroblasts by Electrospun Fibrous Substrates

Migration and Phenotype Control of Human Dermal Fibroblasts by Electrospun Fibrous Substrates

Current status and future outlook of nano‐based systems for burn wound management

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

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