Electrospun Bilayer Composite Vascular Graft with an Inner Layer Modified by Polyethylene Glycol and Haparin to Regenerate the Blood Vessel

Kuang, Haizhu; Yang, Shuofei; Wang, Yao; He, Yuting; Ye, Kaiqiang; Hu, Junfeng; Shen, Wei; Morsi, Yos S.; Lu, Shuyang; Mo, Xiumei

doi:10.1166/jbn.2019.2666

Cited by 21 publications

(9 citation statements)

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“…In order to promote the blood compatibility of nanofibers, coaxial electrospun scaffolds were constructed in which the inner layer was comprised of PLGA/collagen nanofibers modified by mesoporous silica nanoparticles-grafted with PEG and heparin and the outer layer was composed of polyurethane nanofibers to improve mechanical properties. This composite enhanced the proliferation of both endothelial cells and smooth muscle cells by immunostaining of CD31 and alpha-smooth muscle actin (α-SMA) markers after a rabbit carotid artery implantation, respectively [147]. In addition, heparinized nanofibrous scaffolds also showed the ability to promote the proliferation of ECs, and were thus proposed for the production of bioengineered blood vessels [148].…”

Section: Nanofibers As Delivery Systems Of Angiogenic Substancesmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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Angiogenesis (or the development of new blood vessels) is a key event in tissue engineering and regenerative medicine; thus, a number of biomaterials have been developed and combined with stem cells and/or bioactive molecules to produce three-dimensional (3D) pro-angiogenic constructs. Among the various biomaterials, electrospun nanofibrous scaffolds offer great opportunities for pro-angiogenic approaches in tissue repair and regeneration. Nanofibers made of natural and synthetic polymers are often used to incorporate bioactive components (e.g., bioactive glasses (BGs)) and load biomolecules (e.g., vascular endothelial growth factor (VEGF)) that exert pro-angiogenic activity. Furthermore, seeding of specific types of stem cells (e.g., endothelial progenitor cells) onto nanofibrous scaffolds is considered as a valuable alternative for inducing angiogenesis. The effectiveness of these strategies has been extensively examined both in vitro and in vivo and the outcomes have shown promise in the reconstruction of hard and soft tissues (mainly bone and skin, respectively). However, the translational of electrospun scaffolds with pro-angiogenic molecules or cells is only at its beginning, requiring more research to prove their usefulness in the repair and regeneration of other highly-vascularized vital tissues and organs. This review will cover the latest progress in designing and developing pro-angiogenic electrospun nanofibers and evaluate their usefulness in a tissue engineering and regenerative medicine setting.

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Section: Nanofibers As Delivery Systems Of Angiogenic Substancesmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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“…Heparin can then be bound to the amine group with the use of activators such as 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and hydroxysulfosuccinimide (NHS). In a recent study, Kuang et al [137] immobilized heparin onto mesoporous silica nanoparticles (MSN) using PEG as the linker. The MSN-PEG-heparin nanoparticles were then dispersed into a PLGA/collagen solution for electrospinning.…”

Section: Reducing Thrombogenicity By Including An Anticoagulant Moleculementioning

confidence: 99%

Electrospun nanofiber scaffold for vascular tissue engineering

Rickel

Deng

Engebretson

et al. 2021

Materials Science and Engineering: C

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Due to the prevalence of cardiovascular diseases, there is a large need for small diameter vascular grafts that cannot be fulfilled using autologous vessels. Although medium to large diameter synthetic vessels are in use, no suitable small diameter vascular graft has been developed due to the unique dynamic environment that exists in small vessels. To achieve long term patency, a successful tissue engineered vascular graft would need to closely match the mechanical properties of native tissue, be non-thrombotic and non-immunogenic, and elicit the proper healing response and undergo remodeling to incorporate into the native vasculature. Electrospinning presents a promising approach to the development of a suitable tissue engineered vascular graft. This review provides a comprehensive overview of the different polymers, techniques, and functionalization approaches that have been used to develop an electrospun tissue engineered vascular graft.

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“…Furthermore, PEG has also been widely applied for the surface modification of artificial grafts due to its decreasing cytotoxicity and enhanced biocompatibility [ 15 ]. The available literature has indicated that the degradable PLGA/collagen nanofibers, modified by Mesoporous Silica Nanoparticle (MSN) grafting with PEG and heparin, can enhance blood compatibility and cell proliferation to maintain long-term vascular patency [ 16 ]. Another study demonstrated that a Polycaprolactone–Polyethylene Glycol Methyl Ether (PCL-PEGME) mat could induce the endothelial gene CD31 for expression [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Biocompatibility and Endothelial Differentiation Capacity of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites

et al. 2021

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Cardiovascular Diseases (CVDs) such as atherosclerosis, where inflammation occurs in the blood vessel wall, are one of the major causes of death worldwide. Mesenchymal Stem Cells (MSCs)-based treatment coupled with nanoparticles is considered to be a potential and promising therapeutic strategy for vascular regeneration. Thus, angiogenesis enhanced by nanoparticles is of critical concern. In this study, Polyethylene Glycol (PEG) incorporated with 43.5 ppm of gold (Au) nanoparticles was prepared for the evaluation of biological effects through in vitro and in vivo assessments. The physicochemical properties of PEG and PEG–Au nanocomposites were first characterized by UV-Vis spectrophotometry (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFMs). Furthermore, the reactive oxygen species scavenger ability as well as the hydrophilic property of the nanocomposites were also investigated. Afterwards, the biocompatibility and biological functions of the PEG–Au nanocomposites were evaluated through in vitro assays. The thin coating of PEG containing 43.5 ppm of Au nanoparticles induced the least platelet and monocyte activation. Additionally, the cell behavior of MSCs on PEG–Au 43.5 ppm coating demonstrated better cell proliferation, low ROS generation, and enhancement of cell migration, as well as protein expression of the endothelialization marker CD31, which is associated with angiogenesis capacity. Furthermore, anti-inflammatory and endothelial differentiation ability were both evaluated through in vivo assessments. The evidence demonstrated that PEG–Au 43.5 ppm implantation inhibited capsule formation and facilitated the expression of CD31 in rat models. TUNEL assay also indicated that PEG–Au nanocomposites would not induce significant cell apoptosis. The above results elucidate that the surface modification of PEG–Au nanomaterials may enable them to serve as efficient tools for vascular regeneration grafts.

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Electrospun Bilayer Composite Vascular Graft with an Inner Layer Modified by Polyethylene Glycol and Haparin to Regenerate the Blood Vessel

Cited by 21 publications

References 0 publications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Electrospun nanofiber scaffold for vascular tissue engineering

Evaluation of the Biocompatibility and Endothelial Differentiation Capacity of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites

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