Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity

Abstract

In this study, chitosan/poly(ethylene oxide) (PEO)/lauric arginate (LAE) composite nanofibrous films were fabricated via electrospinning. The addition of LAE did not change the physical properties of chitosan/PEO in acetic aqueous solutions, but increased the fluorescent intensity of chitosan by electrostatic interactions, resulting in uniform and bead-free nanofibers with an average diameter of 150 nm. The Fourier transform infrared spectra and thermal analysis indicated that the LAE molecules were homogeneously dispersed within the chitosan/PEO nanofibers. The formation of electrostatic and hydrogen bonding interactions induced by the LAE addition changed the inter- and intramolecular interactions between PEO and chitosan and further affected the mobility of the polymer molecules, leading to the increased crystallinity and decreased melting point. The hydrophilicity of the nanofibrous films was significantly increased by the incorporation of LAE, as indicated by the decreasing water contact angle from 39° to 10°. Meanwhile, the chitosan/PEO/LAE nanofibrous films showed LAE concentration dependent antimicrobial activity against Escherichia coli and Staphylococcus aureus, suggesting enhanced antimicrobial activity. The fluorescent staining experiments demonstrated that the antimicrobial mechanism of the nanofibrous films was cell membrane damage.

Effect of the presence of ethyl lauroyl arginate on the technological properties of edible fish gelatin films

“…Therefore, the distinct cellular envelope should experience distinct types of interactions with antimicrobial agents (Bhawana et al , ). This theory was later corroborated by Deng et al () who evaluated the antibacterial activity of nanofibre films composed of chitosan/poly ethylene oxide and LAE against two pathogenic strains: one Gram‐positive ( S. aureus ) and another Gram‐negative ( E. coli ). They concluded lipopolysaccharides from the cell wall of Gram‐negative bacteria probably have a barrier effect against antimicrobial compounds.…”

An Antimicrobial Peptide-Loaded Chitosan/Polyethylene Oxide Nanofibrous Membrane Fabricated by Electrospinning Technology

“…The amino groups and hydroxyl groups of arginine and histidine in NP10 may enhance this change together with chitosan. According to similar reports by Zupančič et al (2016) and Deng et al (2018), this may be the reason for the change in peak intensity in the FTIR spectrum.…”

“…Besides, CS has hemostatic activity and anti-infective ability (Jin et al, 2002;Fan et al, 2016;Guan et al, 2016;Li et al, 2016). So far, there have been many reports on the successful preparation of CS-PEO nanofiber membranes, such as the addition of lauric arginate (Deng et al, 2018) and ZIF-8 nanoparticles (Kohsari et al, 2016a) for antibacterial and food safety CS-PEO nanofiber membranes. However, there is no report on AMPs and CS/PEO's effective combination to prepare nanofiber membranes (Dart et al, 2019).…”

Improved Antioxidant and Antifungal Activity of Chitosan Derivatives Bearing Urea Groups

“…Chitosan is one of the most abundant renewable resources and is the only cationic polysaccharide in nature . Chitosan‐based biomaterials have attracted increasing attention due to the characteristics of abundant sources, being renewable, low cost, non‐toxicity, and good biocompatibility . However, the commercial application of chitosan is greatly limited due to its dense structure and poor solubility, which is caused by the intramolecular and intermolecular hydrogen bonding of chitosan as well as the rigid crystalline structure .…”