Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering

Law, Jia Xian; Liau, Ling Ling; Saim, Aminuddin Bin; Yang, Ying; Idrus, Ruszymah Bt Hj

doi:10.1007/s13770-017-0075-9

Cited by 176 publications

(116 citation statements)

References 166 publications

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“…However, electrospun collagen shows immediate shrinking, disintegrating, and weak resistance against dissolution regardless of the solvent used for electrospinning . Chemical, physical, or enzymatic crosslinking can improve and regulate both water solubility and resistance to enzymatic degradation . HA shows enzymatic (hyaluronidase) as well as hydrolytic degradation behavior and can take up much water.…”

Section: Resultsmentioning

confidence: 99%

“…[43] Chemical, physical, or enzymatic crosslinking can improve and regulate both water solubility and resistance to enzymatic degradation. [44] HA shows enzymatic (hyaluronidase) as well as hydrolytic degradation behavior [45] and can take up much water. In contrast, PANi is non-biodegradable due to the lack of cleavable moieties.…”

Section: Hydrolytic Swelling Behaviormentioning

confidence: 99%

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Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

Roshanbinfar

Vogt

Ruther

et al. 2019

Adv Funct Materials

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Cardiac tissue engineering is a promising strategy to prevent functional deterioration or even to enhance cardiac function upon myocardial infarction. Here, electrospun fiber mats containing different combinations of electrically conductive polyaniline, collagen, and/or hyaluronic acid are assessed regarding material properties and compatibility with cardiomyocyte attachment and function. Microstructure analysis reveals that collagen fiber mats contain a wide range of fiber diameters after crosslinking (from ≈300 nm to ≈5 µm); all other fiber mats contain fibers in the range of ≈120 to ≈300 nm. Fiber mats exhibit comparable electrical conductivity to and greater mechanical properties than the native human myocardium, which is considered beneficial. Cell–matrix interaction analysis utilizing postnatal rat cardiomyocytes reveals that the fiber mats are non‐cytotoxic and permit cell attachment and contraction. Fiber mats containing collagen (9.89%), hyaluronic acid (1.1%), and polyaniline (PANi, 1.34%) exhibit the most favorable properties with longer contraction time, higher contractile amplitude, and lower beating rates. Improved contraction is accompanied by increased connexin 43 expression. Importantly, this fiber mat is a suitable material for human‐induced pluripotent stem cell–derived cardiomyocytes regarding cytotoxicity, cell attachment, and function. Collectively, these data demonstrate that fiber mats made of collagen, hyaluronic acid, and polyaniline are promising materials for cardiac tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Section: Hydrolytic Swelling Behaviormentioning

confidence: 99%

Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

Roshanbinfar

Vogt

Ruther

et al. 2019

Adv Funct Materials

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“…Higher Col I expression has long been used as an indicator of chondrocyte dedifferentiation. Col I is a fibrilforming collagen found in abundance in many tissues, including the cornea, dermis and tendons, but not articular cartilage (Law et al 2017a(Law et al , 2017b(Law et al , 2016. Here, Col I expression was lower in the HPL groups compared to the FBS group.…”

Section: Discussionmentioning

confidence: 77%

Human Platelet Lysate Promotes Proliferation but Fails to Maintain Chondrogenic Markers of Chondrocytes

Harto¹,

Mahmud²,

Othman³

et al. 2019

JSM

Self Cite

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Traditionally, foetal bovine serum (FBS) is used as a serum supplement for stem cell expansion in vitro. However, it is associated with xenoimmunisation and the transmission of animal pathogens, which may cause harm to stem cell recipients. As a safer alternative, human platelet lysate (HPL) has been introduced for propagating stem cells. Chondrocytes are expanded in vitro for cartilage repair via autologous chondrocyte implantation (ACI). In this study, we compare the efficacy of HPL prepared from expired platelet concentrates with that of FBS for promoting the proliferation and maintenance of the chondrogenic markers of primary human chondrocytes expanded in vitro. Chondrocytes were cultured in F12:Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% HPL, 10% HPL and 10% FBS. The cell morphology, viability and growth rate were examined from passage 1 (P1) to P3. RNA was isolated from P3 cells for quantitative polymerase chain reaction (qPCR) to determine the gene expression level of the chondrogenic, dedifferentiation and hypertrophic markers. HPL promoted chondrocyte proliferation without compromising cell viability. In addition, the chondrocytes cultured with HPL were smaller. However, HPL failed to maintain the chondrogenic markers, except SOX 9 (SRY-box transcription factor 9), which was upregulated, but not significantly. Nonetheless, HPL also suppressed the expression of type X collagen (Col X), a chondrocyte hypertrophic marker. In summary, we demonstrate the benefits of HPL supplementation in human chondrocyte culture, where it enhances cell proliferation and suppresses chondrocyte hypertrophy. In the future, HPL can be used for the large-scale expansion of chondrocytes for ACI. ABSTRAKSecara tradisinya, serum anak lembu (FBS) digunakan untuk pengkulturan sel in vitro. Namun demikian, FBS dikaitkan dengan penolakan oleh sistem imun dan penularan patogen haiwan yang berkemungkinan membawa kemudaratan kepada penerima sel induk. Lisat platelet manusia (HPL) telah diperkenalkan sebagai alternatif yang lebih selamat untuk pengkulturan sel. Sel kondrosit telah dikultur secara in vitro untuk merawat rawan yang rosak melalui teknik implantasi kondrosit autologus (ACI). Pada kajian ini, kami membandingkan keberkesanan HPL yang disediakan daripada platelet pekat tamat tempoh dan FBS dalam merangsang proliferasi dan mengekalkan penanda kondrogenik sel kondrosit manusia yang dikulturkan secara in vitro. Sel kondrosit dikultur dalam medium F12:DMEM yang ditambah dengan 5% HPL, 10% HPL dan 10% FBS. Morfologi, kadar kehidupan dan kadar pertumbuhan sel daripada P1 hingga P3 dikenal pasti. RNA dipencil daripada sel pada P3 untuk qPCR untuk menentukan ekspresi gen penanda-penanda kondrogenik, penyahbezaan dan hipertrofi. Hasil kajian mendapati HPL merangsang proliferasi sel kondrosit tanpa memkompromikan kadar kehidupan sel. Selain itu, sel kondrosit yang dikultur dengan HPL menunjukkan saiz yang lebih kecil. Namun demikian, HPL gagal mengekalkan penanda-penanda kondrogenik kecuali SOX 9 yang meningkat secara t...

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“…In addition, collagen has been reported to be relatively poorly immunogenic, even if used in allogeneic and xenogeneic forms, for example, recombinant human collagen or bovine and porcine collagen. However, mammalian collagen is associated with the risk of disease transmission, for example, bovine spongiform encephalopathy (for a review, see [89][90][91]). This risk can be reduced by the use of fish collagen, which became to be popular in tissue engineering, including skin tissue engineering and wound healing.…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

“…Specifically, it is a mixture of peptides and proteins produced by partial hydrolysis of collagen extracted from the skin, bone, and connective tissue of animals, such as cattle, pigs, chicken, and also fish. Gelatin can be defined as a complex mixtures of oligomers of the α subunits joined by covalent bonds, and intact and partially hydrolyzed α-chains of varying molecular weight (for a review, see [89,92]). Properties of gelatin, including its spinnability, depend on the source of collagen, animal species, age, type of collagen, type of conversion of collagen to gelatin (i.e., acidic vs. basic hydrolysis), and particularly on the conformation of gelatin molecules [108].…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering

Cited by 176 publications

References 166 publications

Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

Human Platelet Lysate Promotes Proliferation but Fails to Maintain Chondrogenic Markers of Chondrocytes

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

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