Electrospun conductive nanofiber yarns for accelerating mesenchymal stem cells differentiation and maturation into Schwann cell-like cells under a combination of electrical stimulation and chemical induction

Electrospun strong, bioactive, and bioabsorbable silk fibroin/poly (L-lactic-acid) nanoyarns for constructing advanced nanotextile tissue scaffolds

“…Controlling and regulating the stem cell differentiation is also significant importance for biomaterial scaffolds. Our previous studies have demonstrated that the nanoyarns themselves could not promote the differentiation of HADMSCs, but the nanoyarns could support the biochemical induction of HADMSC differentiation toward smooth muscle,Schwann cells, osteogenic and tenogenic lineages by using the corresponding differentiation medium [ 4 , 45 , 54 ]. Moreover, our previous study and existing studies from other groups also indicated that the loading of bioactive agents like therapeutic drugs and cytokines into nanoyarns are simple and feasible for inducing the directional differentiation of stem cells [ 32 , 55 , 56 ].…”

Exosomes derived from differentiated human ADMSC with the Schwann cell phenotype modulate peripheral nerve-related cellular functions

“…hADMSCs were differentiated into the SC-like phenotype following our previous protocol with some modification ( Fig. 1 A) [ 21 ]. The morphologies and biomarkers of undifferentiated and differentiated hADMSCs were evaluated after 2-week differentiation.…”

“…It is widely accepted that the SC-like cells differentiated from ADMSCs have a beneficial role for the treatment of peripheral nerve injuries [ 19 , 93 ]. Our previous studies have reported that aligned topography, electrical stimulation, and neural differentiation media promoted hADMSC differentiation towards myelinating SCs [ 21 , 52 ]. In the present study, hADMSC-SCs were achieved by the differentiation of hADMSCs using previously defined induction medium plus BDNF, which has been reported to promote SC marker expressions and myelination [ 94 , 95 ].…”

“…The rSCs were harvested from the sciatic nerves of rats according to the protocol from our previous study [ 21 , 57 ]. The rSCs were then seeded onto a poly- d -Lysine coated T-25 flask and cultured with DMEM medium with 10% FBS and 1% P/S in 5% CO 2 at 37 °C.…”

“…At the damaged site of a peripheral nerve, the implantation of MSCs has been reported to facilitate axonal growth and remyelination as well as enhance angiogenesis and vascularization [ [14] , [15] , [16] , [17] , [18] ]. Notably, MSCs can be differentiated into Schwann cell (SC)-like phenotypes (MSC-SCs) by using cocktail chemical induction, electrical stimulation, or their combinations [ [19] , [20] , [21] ]. Interestingly, several studies have shown that introducing MSC-SCs promoted sciatic nerve regeneration and functional recovery, and the regeneration efficacy with MSC-SCs was superior to that with acellular or MSC counterparts [ [22] , [23] , [24] ].…”