Electrospun Cytocompatible Polycaprolactone Blend Composite with Enhanced Wettability for Bone Tissue Engineering

Chakrapani, Vidhya; Kumar, T.S. Sampath; Raj, Deepa K.; Kumary, T. V.

doi:10.1166/jnn.2017.13713

Cited by 18 publications

(19 citation statements)

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“…Young’s modulus was calculated as the slope of the linear section of the stress–strain curve where the proportional relationship between stress and strain is described by Hooke’s law. All the results obtained are in line with what reported in literature [ 48 , 49 ]. The tensile strength was higher for all the samples when evaluated in the dry state.…”

Section: Resultssupporting

confidence: 93%

Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution

et al. 2020

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Collagen, thanks to its biocompatibility, biodegradability and weak antigenicity, is widely used in dressings and scaffolds, also as electrospun fibers. Its mechanical stability can be improved by adding polycaprolactone (PCL), a synthetic and biodegradable aliphatic polyester. While previously collagen/PCL combinations were electrospun in solvents such as hexafluoroisopropanol (HFIP) or trifluoroethanol (TFE), more recently literature describes collagen/PCL nanofibers obtained in acidic aqueous solutions. A good morphology of the fibers represents in this case still a challenge, especially for high collagen/PCL ratios. In this work, thanks to preliminary rheological and physicochemical characterization of the solutions and to a Design of Experiments (DOE) approach on process parameters, regular and dimensionally uniform fibers were obtained with collagen/PCL ratios up to 1:2 and 1:1 w/w. Collagen ratio appeared relevant for mechanical strength of dry and hydrated fibers. WAXS and FTIR analysis showed that collagen denaturation is related both to the medium and to the electrospinning process. After one week in aqueous environment, collagen release was complete and a concentration dependent stimulatory effect on fibroblast growth was observed, suggesting the fiber suitability for wound healing. The positive effect of collagen on mechanical properties and on fibroblast biocompatibility was confirmed by a direct comparison of nanofiber performance after collagen substitution with gelatin.

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Section: Resultssupporting

confidence: 93%

Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution

et al. 2020

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“…The cytocompatibility and biodegradability of PCL as well as its food and drug administration (FDA) approval and suitable mechanical properties, make it a favorable candidate for bone tissue engineering . However, the hydrophobic nature of PCL is the most important challenge in the initial steps of cell culturing in tissue engineering applications, because it acts as a barrier for cell attachment, proliferation and differentiation . There are abundant techniques to overcome the hydrophobicity of such synthetic polymers.…”

Section: Introductionmentioning

confidence: 99%

“…7 However, the hydrophobic nature of PCL is the most important challenge in the initial steps of cell culturing in tissue engineering applications, because it acts as a barrier for cell attachment, proliferation and differentiation. 8,9 There are abundant techniques to overcome the hydrophobicity of such synthetic polymers. Although Plasma treatment is a common modification method, it can only alter the surface of scaffold and it has a low chemical stability.…”

Section: Introductionmentioning

confidence: 99%

Surfactant‐assisted‐water‐exposed versus surfactant‐aqueous‐solution‐exposed electrospinning of novel super hydrophilic Polycaprolactone‐based fibers: Cell culture studies

Zargarian

Haddadi‐Asl

Azarnia

et al. 2019

J Biomedical Materials Res

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Blend electrospun scaffolds composed of Polycaprolactone and Pluronic are suitable for bone tissue engineering due to their excellent biocompatibility and hydrophilicity. However, exceeding from certain amounts of Pluronic, surface enrichment of this polymer leads to segregation of Pluronic chains within the fiber, endangering the integrity and mechanical properties of the scaffold. In this article, a novel method of blend electrospinning has been employed using a parallel water supply, positioning the Pluronic chains on the surface, thus enhancing the miscibility within the fibers. Water uptake test revealed the super hydrophilicity of obtained scaffolds. Atr‐FTIR and X‐ray photoelectron spectroscopy verified a higher percentage of Pluronics on the surface in comparison with conventional blend electrospinning. Tensile test demonstrated improved mechanical properties of the modified scaffolds. The results of cytocompatibility tests have also revealed enhanced viability of cells on these scaffolds confirming their great promise for clinical applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1204–1212, 2019.

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“…Poly(ɛ‐caprolactone) (PCL) is a semicrystalline aliphatic polyester, has been approved by the U.S. Food and Drug Administration, and is widely studied for TE applications due to its biocompatibility, biodegradability, good mechanical properties, and low cost . However, poor tissue integration of scaffolds made from PCL due to its hydrophobic nature and lack of cell‐binding motifs in the chemical structure of PCL are major challenges for application of PCL in TE .…”

Section: Introductionmentioning

confidence: 99%

“…However, poor tissue integration of scaffolds made from PCL due to its hydrophobic nature and lack of cell‐binding motifs in the chemical structure of PCL are major challenges for application of PCL in TE . Surface modification using different physical and chemical methods such as plasma treatment, alkali treatment, radio frequency, functionalization with different biomolecules, and immobilized proteins are the most common approaches to overcome the drawbacks of PCL scaffolds . Immobilization of biological molecules such as proteins, peptides, or polysaccharides on the surface of PCL scaffolds is a promising way to improve the biocompatibility of PCL scaffolds for TE applications .…”

Section: Introductionmentioning

confidence: 99%

Immobilization of silk fibroin on the surface of PCL nanofibrous scaffolds for tissue engineering applications

Khosravi

Ghasemi‐Mobarakeh

Mollahosseini

et al. 2018

J of Applied Polymer Sci

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Poly(E-caprolactone) (PCL) is explored in tissue engineering (TE) applications due to its biocompatibility, processability, and appropriate mechanical properties. However, its hydrophobic nature and lack of functional groups in its structure are major drawbacks of PCL-based scaffolds limiting appropriate cell adhesion and proliferation. In this study, silk fibroin (SF) was immobilized on the surface of electrospun PCL nanofibers via covalent bonds in order to improve their hydrophilicity. To this end, the surface of PCL nanofibers was activated by ultraviolet (UV)-ozone irradiation followed by carboxylic functional groups immobilization on their surface by their immersion in acrylic acid under UV radiation and final immersion in SF solution. Furthermore, morphological, mechanical, contact angle, and Attenuated total reflection-Fourier transform infrared (ATR-FTIR) were measured to assess the properties of the surfacemodified PCL nanofibers grafted with SF. ATR-FTIR results confirmed the presence of SF on the surface of PCL nanofibers. Moreover, contact angle measurements of the PCL nanofibers grafted with SF showed the contact angle of zero indicating high hydrophilicity of modified nanofibers. In vitro cell culture studies using NIH 3T3 mouse fibroblasts confirmed enhanced cytocompatibility, cell adhesion, and proliferation of the SF-treated PCL nanofibers.Silk fibroin (SF) is a naturally occurring polymer, derived from Bombyx mori silkworm has been used in TE, due to its biocompatibility, low immunoreactivity, biodegradability, suitable oxygen and water vapor permeability, and good mechanical properties. [14][15][16][17][18][19][20] Moreover, SF improves cell adhesion and proliferation for TE applications. 14,16 Combination of the two aforementioned polymers (PCL and SF) can be considered as a synthetic/natural polyblend to

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Electrospun Cytocompatible Polycaprolactone Blend Composite with Enhanced Wettability for Bone Tissue Engineering

Cited by 18 publications

References 0 publications

Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution

Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution

Surfactant‐assisted‐water‐exposed versus surfactant‐aqueous‐solution‐exposed electrospinning of novel super hydrophilic Polycaprolactone‐based fibers: Cell culture studies

Immobilization of silk fibroin on the surface of PCL nanofibrous scaffolds for tissue engineering applications

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