Electrospun electroconductive constructs of aligned fibers for cardiac tissue engineering

Mancino, Chiara; Hendrickson, Troy; Whitney, Lauren V.; Paradiso, Francesca; Abasi, Sara; Tasciotti, Ennio; Taraballi, Francesca; Guiseppi‐Elie, Anthony

doi:10.1016/j.nano.2022.102567

Cited by 13 publications

(9 citation statements)

References 59 publications

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“…Quantification of fiber alignment at different rotation speeds was achieved using FIJI's directionality plug-in to calculate the coefficient of alignment (COA) for each electrospinning condition using the equation COA = good of fit × amount in which "goodness of fit" and "amount" were obtained from the FIJI directionality plugin. 48 Nonaligned fibers and fibers fabricated at low rotation speeds of 500 and 1000 rpm demonstrated COA values of 0.19, 0.04, and 0.40, respectively; interestingly, the 500 rpm sample COA value was significantly lower than that observed with the static collector, suggesting that slow rotation can actually enhance the randomness of fiber deposition. We hypothesize this result may be attributed to the increased the available surface area for fiber collection on the rotating collector, which may dilute the influence of predeposited fibers in influencing local fiber orientation during collection.…”

Section: ■ Results and Discussionmentioning

confidence: 89%

“…An increase in drum rotation speed led to an increase in the degree of fiber alignment (Figure A and B; see Supporting Information, Figure S3 for additional representative images). Quantification of fiber alignment at different rotation speeds was achieved using FIJI’s directionality plug-in to calculate the coefficient of alignment (COA) for each electrospinning condition using the equation COA = good of fit × amount in which “goodness of fit” and “amount” were obtained from the FIJI directionality plugin . Nonaligned fibers and fibers fabricated at low rotation speeds of 500 and 1000 rpm demonstrated COA values of 0.19, 0.04, and 0.40, respectively; interestingly, the 500 rpm sample COA value was significantly lower than that observed with the static collector, suggesting that slow rotation can actually enhance the randomness of fiber deposition.…”

Section: Resultsmentioning

confidence: 99%

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Reactive Cell Electrospinning of Anisotropically Aligned and Bilayer Hydrogel Nanofiber Networks

Dawson,

Xu,

Hoare

2023

ACS Biomater. Sci. Eng.

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Structured hydrogels that incorporate aligned nanofibrous morphologies have been demonstrated to better replicate the structures of native extracellular matrices and thus their function in guiding cell responses. However, current techniques for nanofiber fabrication are limited in their ability to create hydrogel scaffolds with tunable directional alignments and cell types/densities, as required to reproduce more complex native tissue structures. Herein, we leverage a reactive cell electrospinning technique based on the dynamic covalent cross-linking of poly(ethylene glycol methacrylate (POEGMA) precursor polymers to fabricate aligned hydrogel nanofibers that can be directly loaded with cells during the electrospinning process. The scaffolds were found to support high C2C12 myoblast viabilities greater than 85% over 14 days, with changes in the electrospinning collector allowing for the single-step fabrication of nonaligned, aligned, or cross-aligned nanofibrous networks. Cell aspect ratios on aligned scaffolds were found on average to be 27% higher compared to those on nonaligned scaffolds; furthermore, cell-loaded bilayer scaffolds with perpendicular fiber alignments showed evidence of enabling localized directional cell responses to individual layer fiber directions while avoiding delamination between the layers. This fabrication approach thus offers potential for better mimicking the structure and thus function of aligned and multilayered tissues (e.g., smooth muscle, neural, or tendon tissues).

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Section: ■ Results and Discussionmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Reactive Cell Electrospinning of Anisotropically Aligned and Bilayer Hydrogel Nanofiber Networks

Dawson,

Xu,

Hoare

2023

ACS Biomater. Sci. Eng.

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“…Building upon the results obtained from the HPLC analysis and aiming for a more sustained release of antibiotics for a longer period of time, we added an additional layer of PLGA, using it as a coating. The PLGA deposition was carried out as described in a previous work from our group. , The nanofibrous coating in PLGA was electrospun on top of the nanofibrous mats already made obtaining a homogeneous deposition with fibers of 177.3 ± 19.8 nm (mean ± SD) in diameter (Figure S4). HPLC evaluation was carried out using the same method as for noncoated mats.…”

Section: Resultsmentioning

confidence: 99%

“…The PLGA deposition was carried out as described in a previous work from our group. 106 , 107 The nanofibrous coating in PLGA was electrospun on top of the nanofibrous mats already made obtaining a homogeneous deposition with fibers of 177.3 ± 19.8 nm (mean ± SD) in diameter ( Figure S4 ). HPLC evaluation was carried out using the same method as for noncoated mats.…”

Section: Resultsmentioning

confidence: 99%

Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin

Serpelloni,

Williams,

Caserta

et al. 2024

ACS Omega

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As the population ages, the number of vascular surgery procedures performed increases. Older adults often have multiple comorbidities, such as diabetes and hypertension, that increase the risk of complications from vascular surgery including vascular graft infection (VGI). VGI is a serious complication with significant morbidity, mortality, and healthcare costs. Here, we aimed to develop a nanofibrous chitosan-based coating for vascular grafts loaded with different concentrations of the vancomycin antibiotic vancomycin (VAN). Blending chitosan with poly(vinyl alcohol) or poly(ethylene oxide) copolymers improved solubility and ease of spinning. Thermal gravimetric analysis and Fourier transform infrared spectroscopy confirmed the presence of VAN in the nanofibrous membranes. Kinetics of VAN release from the nanofibrous mats were evaluated using highperformance liquid chromatography, showing a burst followed by sustained release over 24 h. To achieve longer sustained release, a poly(lactic-co-glycolic acid) coating was applied, resulting in extended release of up to 7 days. Biocompatibility assessment using human umbilical vein endothelial cells demonstrated successful attachment and viability of the nanofiber patches. Our study provides insights into the development of a drug delivery system for vascular grafts aimed at preventing infection during implantation, highlighting the potential of electrospinning as a promising technique in the field of vascular surgery.

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“…As a result, functional coatings have been applied on PLGA scaffolds to increase cell adhesion and viability. These coating materials include polymers such as polyethylene glycol (PEG) [ 7 ], polydopamine (PDA), polypyrrole (Ppy) [ 8 ], poly-lysine [ 9 ], polyethyleneimine (PEI) [ 10 ], and ECM components such as HA [ 11 ], laminin [ 12 ], FN [ 13 ], and collagen [ 14 ]. Studies have shown that modifying PLGA-based scaffolds with these materials could improve the hydrophilicity and cell adhesion on PLGA.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification Progress for PLGA-Based Cell Scaffolds

Yan,

Hua,

Wang

et al. 2024

Polymers

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Poly(lactic-glycolic acid) (PLGA) is a biocompatible bio-scaffold material, but its own hydrophobic and electrically neutral surface limits its application as a cell scaffold. Polymer materials, mimics ECM materials, and organic material have often been used as coating materials for PLGA cell scaffolds to improve the poor cell adhesion of PLGA and enhance tissue adaptation. These coating materials can be modified on the PLGA surface via simple physical or chemical methods, and coating multiple materials can simultaneously confer different functions to the PLGA scaffold; not only does this ensure stronger cell adhesion but it also modulates cell behavior and function. This approach to coating could facilitate the production of more PLGA-based cell scaffolds. This review focuses on the PLGA surface-modified materials, methods, and applications, and will provide guidance for PLGA surface modification.

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Electrospun electroconductive constructs of aligned fibers for cardiac tissue engineering

Cited by 13 publications

References 59 publications

Reactive Cell Electrospinning of Anisotropically Aligned and Bilayer Hydrogel Nanofiber Networks

Reactive Cell Electrospinning of Anisotropically Aligned and Bilayer Hydrogel Nanofiber Networks

Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin

Surface Modification Progress for PLGA-Based Cell Scaffolds

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