Electrospun F18 Bioactive Glass/PCL—Poly (ε-caprolactone)—Membrane for Guided Tissue Regeneration

Abstract

Barrier membranes that are used for guided tissue regeneration (GTR) therapy usually lack bioactivity and the capability to promote new bone tissue formation. However, the incorporation of an osteogenic agent into polymeric membranes seems to be the most assertive strategy to enhance their regenerative potential. Here, the manufacturing of composite electrospun membranes made of poly (ε-caprolactone) (PCL) and particles of a novel bioactive glass composition (F18) is described. The membranes were mechanically and biologically tested with tensile strength tests and tissue culture with MG-63 osteoblast-like cell line, respectively. The PCL-F18 composite membranes demonstrated no increased cytotoxicity and an enhanced osteogenic potential when compared to pure PCL membranes. Moreover, the addition of the bioactive phase increased the membrane tensile strength. These preliminary results suggested that these new membranes can be a strong candidate for small bone injuries treatment by GTR technique.

Creation of a unique architectural structure of bioactive glass sub‐micron particles incorporated in a polycaprolactone/gelatin fibrous mat; characterization, bioactivity, and cellular evaluations

“…This suggests the important role of ionic release on cellular behavior. 7 In addition, based on previous studies, it is believed that matrix mineralization is associated with the release of BG ionic products which are known to stimulate osteogenic differentiation hDPSC 7,8,12,26 ; however, this requires further evaluation to confirm this finding.…”

“…Therefore, the incorporation of BG particles into a PCL/GT polymer matrix could present the advantage of combining bioactivity, therapeutic ionic release and osteogenic properties of BGs with ideal mechanical and physical properties of the PCL/GT. 9 To date, some studies have been carried out on BG contained PCL [10][11][12] and PCL/GT 13 fibers. However, the main challenge associated with the produced fibers was the incorporation of irregular morphology and wide size range distribution of BG particles, which led to a lower HA deposition rate.…”

Caracterização estrutural e morfológica de compostos químicos para produção vítrea

“…Com o avanço da ciência e a profunda compreensão das propriedades físicas e químicas dos vidros acoplados a modernas tecnologias analíticas e de controle, fizeram com que os estudos sobre este material se intensificasse e com isso novos avanços tecnológicos pudessem ser realizados, estes voltados para resolver problemas mais urgentes do mundo, como na área da saúde, energia e águas [9,10].…”

Composite Membranes of Poly(ε-caprolactone) with Bisphosphonate-Loaded Bioactive Glasses for Potential Bone Tissue Engineering Applications

“…Compared with the most commonly used PLA and PLGA, PCL doesn’t release acidic degradation products that could affect cell growth [4]. Although PCL has many advantages, only a handful of studies have been published concerning PCL based membranes for GTR/GBR [8,9,10,11,12,13,14,15], as it shows low bioactivity, high hydrophobicity and weak mechanical properties that limit its effectiveness [15,16].…”

“…A commonly used method to increase bioactivity, alter the degradation rate, improve mechanical properties and enhance cell adhesion and proliferation on polymeric surfaces intended for tissue engineering applications including GTR/GBR [11,15,17,18,19,20,21,22,23,24,25,26,27,28,29,30], is the incorporation of bioactive inorganic fillers, like hydroxyapatite, tricalcium phosphate or bioglasses (BGs) [31,32,33,34,35,36,37]. BGs consist of a silicon dioxide-based network modified by other metal oxides such as Na 2 O, CaO and P 2 O 5 [38].…”