Electrospun fiber-mediated delivery of neurotrophin-3 mRNA for neural tissue engineering applications

Puhl, Devan L; Funnell, Jessica L; Fink, Tanner D.; Swaminathan, Anuj; Oudega, Martin; Zha, R. Helen; Gilbert, Ryan J.

doi:10.1016/j.actbio.2022.11.025

Cited by 13 publications

(11 citation statements)

References 125 publications

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“…It would be hypothesized that based on its physicochemical properties, NGF would be capable of coassembling with SF in a manner similar to LYS or BLAC and exhibit similar bolus release profiles. The DRG explant model was chosen, as it is routinely used to investigate neurite outgrowth, ,− which is highly indicative of nerve regeneration.…”

Section: Resultsmentioning

confidence: 99%

One-Pot Assembly of Drug-Eluting Silk Coatings with Applications for Nerve Regeneration

Fink,

Funnell,

Gilbert

et al. 2023

ACS Biomater. Sci. Eng.

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Clinical use of polymeric scaffolds for tissue engineering often suffers from their inability to promote strong cellular interactions. Functionalization with biomolecules may improve outcomes; however, current functionalization approaches using covalent chemistry or physical adsorption can lead to loss of biomolecule bioactivity. Here, we demonstrate a novel bottom-up approach for enhancing the bioactivity of poly(L-lactic acid) electrospun scaffolds though interfacial coassembly of protein payloads with silk fibroin into nanothin coatings. In our approach, protein payloads are first added into an aqueous solution with Bombyx mori-derived silk fibroin. Phosphate anions are then added to trigger coassembly of the payload and silk fibroin, as well as noncovalent formation of a payload-silk fibroin coating at poly(Llactic) acid fiber surfaces. Importantly, the coassembly process results in homogeneous distribution of protein payloads, with the loading quantity depending on payload concentration in solution and coating time. This coassembly process yields greater loading capacity than physical adsorption methods, and the payloads can be released over time in physiologically relevant conditions. We also demonstrate that the coating coassembly process can incorporate nerve growth factor and that coassembled coatings lead to significantly more neurite extension than loading via adsorption in a rat dorsal root ganglia explant culture model.

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Section: Resultsmentioning

confidence: 99%

One-Pot Assembly of Drug-Eluting Silk Coatings with Applications for Nerve Regeneration

Fink,

Funnell,

Gilbert

et al. 2023

ACS Biomater. Sci. Eng.

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“…In 2023, for the first time, Puhl et al delivered mRNA encoding NT-3 from the aligned electrospun PLLA fibers for peripheral nerve regeneration [57]. In this approach, the PLLA fiber's surface was functionalized with poly(3,4-dihydroxy-Lphenylalanine) (pDOPA) or coated with dextran sulfate sodium salt (DSS).…”

Section: Nerve Tissue Regenerationmentioning

confidence: 99%

Development of Drug-Delivery Textiles Using Different Electrospinning Techniques: A Review

C. Gouveia,

Mouro

2024

Electrospinning - Theory, Applications, and Update Challenges

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Electrospinning, a remarkable and versatile technique has been related to medical textiles, aiming to produce nanomaterials for drug delivery and tissue regeneration applications. Furthermore, electrospun nanofibrous materials with unique properties as favorable pore size distribution, porosity, surface area, and wettability, along with effective mechanical properties, are the frontrunner solutions. Also, the features of the nanofibrous structures can be designed and optimized by controlling electrospinning parameters related to the solution properties, the setup parameters, and the environmental conditions to design nanofibrous textile materials for the desired applications. Further, to accomplish the required functionality of the drug-delivery systems, a rather broad range of drugs have been loaded into the nanofibers using different electrospinning techniques, namely the blending, side-by-side, coaxial, tri-axial, emulsion, and multi-needle electrospinning, in order to accomplish specific drug-release profiles of the designed nanofibrous textiles. Thus, this chapter describes the different electrospinning techniques that have been utilized in the production of the textile nanofibrous materials as the application of these materials in bone, nerve, periodontal, and vascular regeneration, as well as in wound dressings, personal-protective-equipment (PPE), and cancer treatment, providing an overview of the recent studies and highlighting the current challenges and future perspectives for their medical applications.

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“…Thus, nerve repair drugs can be loaded; the pore structure can promote diffusion of nerve repair drugs or growth factors. Puhl et al reported an aligned fiber nerve conduit using a pseudouridine-modified neurotrophin-3 (NT-3) mRNA delivery system [ 45 ]. The results showed that electrospun fiber conduits could provide ideal structural support and sustained drug-release properties for peripheral nerve regeneration.…”

Section: Fabrication Strategiesmentioning

confidence: 99%

The Porous Structure of Peripheral Nerve Guidance Conduits: Features, Fabrication, and Implications for Peripheral Nerve Regeneration

Wan,

Wang,

Zhang

et al. 2023

IJMS

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Porous structure is an important three-dimensional morphological feature of the peripheral nerve guidance conduit (NGC), which permits the infiltration of cells, nutrients, and molecular signals and the discharge of metabolic waste. Porous structures with precisely customized pore sizes, porosities, and connectivities are being used to construct fully permeable, semi-permeable, and asymmetric peripheral NGCs for the replacement of traditional nerve autografts in the treatment of long-segment peripheral nerve injury. In this review, the features of porous structures and the classification of NGCs based on these characteristics are discussed. Common methods for constructing 3D porous NGCs in current research are described, as well as the pore characteristics and the parameters used to tune the pores. The effects of the porous structure on the physical properties of NGCs, including biodegradation, mechanical performance, and permeability, were analyzed. Pore structure affects the biological behavior of Schwann cells, macrophages, fibroblasts, and vascular endothelial cells during peripheral nerve regeneration. The construction of ideal porous structures is a significant advancement in the regeneration of peripheral nerve tissue engineering materials. The purpose of this review is to generalize, summarize, and analyze methods for the preparation of porous NGCs and their biological functions in promoting peripheral nerve regeneration to guide the development of medical nerve repair materials.

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Electrospun fiber-mediated delivery of neurotrophin-3 mRNA for neural tissue engineering applications

Cited by 13 publications

References 125 publications

One-Pot Assembly of Drug-Eluting Silk Coatings with Applications for Nerve Regeneration

One-Pot Assembly of Drug-Eluting Silk Coatings with Applications for Nerve Regeneration

Development of Drug-Delivery Textiles Using Different Electrospinning Techniques: A Review

The Porous Structure of Peripheral Nerve Guidance Conduits: Features, Fabrication, and Implications for Peripheral Nerve Regeneration

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