Electrospun fibrous mats from a l-phenylalanine based poly(ester amide): Drug delivery and accelerated degradation by loading enzymes

Murase, Sara K.; Valle, Luís J. del; Kobauri, Sophio; Katsarava, Ramaz; Puiggalı́, Jordi

doi:10.1016/j.polymdegradstab.2015.05.018

Cited by 17 publications

(15 citation statements)

References 37 publications

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“…49 However, some proteins withstand both denaturation factors and retain their functionality aer electrospinning. These proteins include insulin, 50 chondroitinase, 9 lysozyme, 51,52 chymotrypsin, 53,54 and others.…”

Section: Bsa Release From the Blended Matsmentioning

confidence: 99%

The miscibility and spatial distribution of the components in electrospun polymer–protein mats

et al. 2020

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Section: Bsa Release From the Blended Matsmentioning

confidence: 99%

The miscibility and spatial distribution of the components in electrospun polymer–protein mats

et al. 2020

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“…For example, PEAs have been used as cell scaffolds for tissue regeneration and were found to support cell adhesion and proliferation [40,41]. They have also been explored for their ability to encapsulate and release cell growth factors and bactericides [42,43]. PEA particles loaded with celecoxib were shown to release the drug in response to inflammation and were well tolerated in a rat model [27].…”

Section: Introductionmentioning

confidence: 99%

GSK3787-Loaded Poly(Ester Amide) Particles for Intra-Articular Drug Delivery

et al. 2020

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Osteoarthritis (OA) is a debilitating joint disorder affecting more than 240 million people. There is no disease modifying therapeutic, and drugs that are used to alleviate OA symptoms result in side effects. Recent research indicates that inhibition of peroxisome proliferator-activated receptor δ (PPARδ) in cartilage may attenuate the development or progression of OA. PPARδ antagonists such as GSK3787 exist, but would benefit from delivery to joints to avoid side effects. Described here is the loading of GSK3787 into poly(ester amide) (PEA) particles. The particles contained 8 wt.% drug and had mean diameters of about 600 nm. Differential scanning calorimetry indicated the drug was in crystalline domains in the particles. Atomic force microscopy was used to measure the Young’s moduli of individual particles as 2.8 MPa. In vitro drug release studies showed 11% GSK3787 was released over 30 days. Studies in immature murine articular cartilage (IMAC) cells indicated low toxicity from the drug, empty particles, and drug-loaded particles and that the particles were not taken up by the cells. Ex vivo studies on murine joints showed that the particles could be injected into the joint space and resided there for at least 7 days. Overall, these results indicate that GSK3787-loaded PEA particles warrant further investigation as a delivery system for potential OA therapy.

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“…The potential of PEAs for electrospinning has been further demonstrated with the development of synthetic elastomeric scaffolds with tunable mechanical and physical properties [22]. Despite all the advantages in using PEAs for biomedical applications, especially for soft tissue engineering, the processing methods employed to create artificial 3D PEA-based ECMs are still limited to electrospinning, freeze-drying, solvent casting and microfabrication [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

The influence of poly(ester amide) on the structural and functional features of 3D additive manufactured poly(ε-caprolactone) scaffolds

Gloria

Frydman

Lamas

et al. 2019

Materials Science and Engineering: C

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The current research reports for the first time the use of blends of poly(ε-caprolactone) (PCL) and poly(ester amide) (PEA) for the fabrication of 3D additive manufactured scaffolds. Tailor made PEA was synthesized to afford fully miscible blends of PCL and PEA using different percentages (5, 10, 15 and 20% w/w). Stability, characteristic temperatures and material's compatibility were studied through thermal analyses (i.e., TGA, DSC). Even though DMTA and static compression tests demonstrated the possibility to improve the storage modulus, Young's modulus and maximum stress by increasing the amount of PEA, a decrease of hardness was found beyond a threshold concentration of PEA as the lowest values were achieved for PCL/PEA (20% w/w) scaffolds (from 0.39±0.03 GPa to 0.21±0.02 GPa in the analysed load range). The scaffolds presented a controlled morphology and a fully interconnected network of internal channels. The water contact angle measurements showed a clear increase of hydrophilicity resulting from the addition of PEA. This result was further corroborated with the improved adhesion and proliferation of human mesenchymal stem cells (hMSCs). The presence of PEA also influenced the cell morphology. Better cell spreading and a much higher and homogenous number of cells were observed for PCL/PEA scaffolds when compared to PCL ones.

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Electrospun fibrous mats from a l-phenylalanine based poly(ester amide): Drug delivery and accelerated degradation by loading enzymes

Cited by 17 publications

References 37 publications

The miscibility and spatial distribution of the components in electrospun polymer–protein mats

The miscibility and spatial distribution of the components in electrospun polymer–protein mats

GSK3787-Loaded Poly(Ester Amide) Particles for Intra-Articular Drug Delivery

The influence of poly(ester amide) on the structural and functional features of 3D additive manufactured poly(ε-caprolactone) scaffolds

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