Electrospun gelatin/poly(ε-caprolactone) fibrous scaffold modified with calcium phosphate for bone tissue engineering

Rajzer, I.; Menaszek, E.; Kwiatkowski, Ryszard; Planell, Josep A.; Castaño, Óscar

doi:10.1016/j.msec.2014.08.017

Cited by 131 publications

(68 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the primary drawback is that the acidic degradation products may cause tissue inflammation in the degradation process [15,16,17]. Furthermore, the innate hydrophobicity of these macromolecules leads to a decrease in surface wettability, which is unfavorable for maintaining original molecular conformation and bioactivity of loaded drugs [13,18,19]. The natural polymers usually used as carriers for bFGF are collagen [20] and gelatin [21].…”

Section: Introductionmentioning

confidence: 99%

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Wang

Niu

et al. 2018

Materials

View full text Add to dashboard Cite

Basic fibroblast growth factor (bFGF) plays a significant role in stimulating cell proliferation. It remains a challenge in the field of biomaterials to develop a carrier with the capacity of continuously releasing bioactive bFGF. In this study, porous bFGF-loaded silk fibroin (SF) microspheres, with inside-out channels, were fabricated by high-voltage electrostatic differentiation, and followed by lyophilization. The embedded bFGF exhibited a slow release mode for over 13 days without suffering burst release. SEM observations showed that incubated L929 cells could fully spread and produce collagen-like fibrous matrix on the surface of SF microspheres. CLSM observations and the results of cell viability assay indicated that bFGF-loaded microspheres could significantly promote cell proliferation during five to nine days of culture, compared to bFGF-unloaded microspheres. This reveals that the bFGF released from SF microspheres retained obvious bioactivity to stimulate cell growth. Such microspheres sustainably releasing bioactive bFGF might be applied to massive cell culture and tissue engineering as a matrix directly, or after being combined with three-dimensional scaffolds.

show abstract

Section: Introductionmentioning

confidence: 99%

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Wang

Niu

et al. 2018

Materials

View full text Add to dashboard Cite

show abstract

“…mechanical property[52]. Hickey et al studied the effects of magnesium oxide (MgO) nanoparticles added with HA nanoparticle-poly (L-lactic acid) (PLLA) composites for orthopedic tissue engineering[53].…”

mentioning

confidence: 99%

Controlled release of drugs in electrosprayed nanoparticles for bone tissue engineering

Jayaraman

Gandhimathi

Venugopal

et al. 2015

Advanced Drug Delivery Reviews

123

View full text Add to dashboard Cite

“…According to some studies, hydrophilic matrices/scaffolds may improve cell affinity, cell proliferation, wound healing, and cell spreading/migration events when compared to hydrophobic ones [36–40]. Interestingly, Rajzer et al [41] recently demonstrated that, despite PCL scaffolds acting as a mechanically strong skeleton for bone tissue regeneration, the incorporation of gelatin into the PCL matrix resulted in enhanced cell spreading and mineralization activity. Notably, the gelatin-based scaffold prepared in that study was constituted from calcium phosphate nanoparticles, thus the positive cell response obtained could also be due to the presence of nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, the gelatin-based scaffold prepared in that study was constituted from calcium phosphate nanoparticles, thus the positive cell response obtained could also be due to the presence of nanoparticles. Nevertheless, the combination of gelatin and PCL has been truly revealed as an effective matrix for the preparation of biomimetic scaffolds applied in bone tissue engineering [41, 42], which may, in turn, be intrinsically related to the gain in wettability seen with gelatin incorporation. However, equilibrium between hydrophobicity and hydrophilicity is essential in order to produce physico-mechanically stable matrices.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering

et al. 2015

View full text Add to dashboard Cite

Objectives To synthesize and characterize biodegradable polymer-based matrices loaded with CaO-nanoparticles for osteomyelitis treatment and bone tissue engineering. Materials and methods Poly(ε-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier Transformed Infrared (FTIR), Energy Dispersive X-ray Spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus (S. aureus) were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. Results CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the 0addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0°). Increasing CaO concentrations progressively reduced the mechanical properties (p≤0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Conclusions Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. Clinical significance CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.

show abstract

Electrospun gelatin/poly(ε-caprolactone) fibrous scaffold modified with calcium phosphate for bone tissue engineering

Cited by 131 publications

References 37 publications

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Controlled release of drugs in electrosprayed nanoparticles for bone tissue engineering

Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering

Contact Info

Product

Resources

About