Electrospun microfiber meshes of silicon-doped vaterite/poly(lactic acid) hybrid for guided bone regeneration

Obata, Akiko; Hotta, Toshiki; Wakita, Takashi; Ota, Yoshio; Kasuga, Toshihiro

doi:10.1016/j.actbio.2009.11.013

Cited by 89 publications

(89 citation statements)

References 34 publications

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“…With the healing of the fracture, the PLLA/n-HA composites would gradually degrade in vivo, facilitating the penetration of the cells and later new bone formation [16].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold

Huang

Xiong

Liu

et al. 2015

BME

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Abstract.To determine the optimal ratio of nano-hydroxyapatite (n-HA) to polylactic acid (PLLA) in the novel three-dimensional porous PLLA/n-HA composite scaffolds, low-temperature rapid prototyping technology was employed to fabricate the composite materials with different n-HA contents. Mechanical properties and degradation behaviors of the composites were examined, and the scaffold microstructure and n-HA dispersion were observed by scanning electron microscope (SEM). Mechanical tests demonstrated that the tensile strength of the composite material gradually decreased with an increase in n-HA content. When the n-HA content reached 20 wt%, the bending strength of the composite material peaked at 138.5 MPa. SEM images demonstrated that the optimal content of n-HA was 20 wt% as the largest interconnected pore size that can be seen, with a porosity as high as 80%. In vitro degradation experiments demonstrated that the pH value of the material containing solution gradually decreased in a time-dependent manner, with a simultaneous weakening of the mechanical properties. In vitro study using rat osteoblast cells showed that the composite scaffolds were biocompatible; the 20 wt% n-HA scaffold offered particular improvement to rat osteoblast cell adhesion and proliferation compared to other compositions. It was therefore concluded that 20 wt% n-HA is the optimal nano-hydroxyapatite (n-HA) to polylactic acid (PLLA) ratio, with promise for bone tissue engineering.

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“…With the healing of the fracture, the PLLA/n-HA composites would gradually degrade in vivo, facilitating the penetration of the cells and later new bone formation [16].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold

Huang

Xiong

Liu

et al. 2015

BME

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“…Keeting et al have demonstrated that the siliconcontaining compound zeolite A (ZA) promotes proliferation, alkaline phosphatase (ALP) activity, and osteocalcin production of human osteoblast-like cells 8) . Recently, Obata et al 12) developed a bi-layered siliconreleasable microfiber mesh with possible applicability for GBR, consisting of a siloxane-PLA-vaterite (Si-PVH) microfiber mesh and a PLA microfiber mesh, using an electrospinning method. As PLA is reported to be highly biocompatible, the PLA side of the bi-layered mesh comes into contact with connective tissue, while the Si-PVH side faces bone tissue to promote proliferation and differentiation of osteoblasts in bone defects.…”

Section: Introductionmentioning

confidence: 99%

Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration

Takeuchi

Machigashira²,

Yamashita³

et al. 2011

Dent. Mater. J.

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A bi-layered silicon-releasable membrane consisting of a siloxane-poly(lactic acid) (PLA)-vaterite hybrid material (Si-PVH) microfiber mesh and a PLA microfiber mesh has been developed by an electrospinning method for guided bone regeneration (GBR) application. The bi-layered membrane was modified to a three-laminar structure by sandwiching an additional PLA microfiber mesh between the Si-PVH and PLA microfiber meshes (Si-PVH/PLA membrane). In this study, the influence of gamma irradiation, used for sterilization, on biological properties of the Si-PVH/PLA membrane was evaluated with osteoblasts and fibroblasts. After gamma irradiation, while the average molecular weight of the Si-PVH/PLA membrane decreased, the Si-PVH/PLA membrane promoted cell proliferation and differentiation (alkaline phosphatase activity and calcification) of osteoblasts, compared with the poly(lactide-co-glycolide) membrane. These results suggest that the gamma-irradiated Si-PVH/PLA membrane is biocompatible with both fibroblasts and osteoblasts, and may have an application for GBR.

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“…Numerous studies have shown the enhanced cellular attachment and proliferation on fibrous materials [15][16][17]. The present authors have successfully prepared a SiPVH fibermat by an electrospinning method and then performed in vivo experiments by placing the fibermat with 8 mm defects in the front midline of the calvaria of New Zealand rabbits [18,19]. They found enhanced bone formation on SiPVH fibermats.…”

Section: Introductionmentioning

confidence: 77%

“…SiV particles of 1 μm diameter, containing 2.6 wt% silicon, were prepared by a carbonation method using methanol and γ-aminopropyltriethoxysilane (APTES; Momentive Performance Materials, Japan) as the silica precursor, as described in our previous works [9,18,19]. One hundred and fifty grams of Ca(OH) 2 , 60 mL of APTES and 2 L of methanol were mixed by bubbling a CO 2 gas in the resulting mixture for 75 min at a rate of 2 L·min −1 .…”

Section: Preparation Of Sipvh Fibermatsmentioning

confidence: 99%

“…The fibermats were prepared by electrospinning at a voltage of 20 kV using the conditions and compositions previously found to be optimal for preparing microfibers of ∼10 μm diameter [18,19]. The flow rate of the chloroform solution was 50 μL·min −1 , and the distance between the nozzle and the collector was 150 mm.…”

Section: Preparation Of Sipvh Fibermatsmentioning

confidence: 99%

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Aluminum Silicate Nanotube Coating of Siloxane‐Poly(lactic acid)‐Vaterite Composite Fibermats for Bone Regeneration

Yamazaki

Maeda

Obata

et al. 2012

Journal of Nanomaterials

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In our earlier work, a flexible fibermat consisting of a biodegradable composite with soluble silicate species, which has been reported to enhance bone formation, was prepared successfully using poly(l-lactic acid) and siloxane-containing calcium carbonate particles by electrospinning. The fibermat showed enhanced bone formation in an in vivo test. In the present work, to improve the hydrophilicity of skeletal fibers in a fibermat, they were coated with nanotubular aluminum silicate crystals, which have a hydrophilic surface that has excellent affinity to body fluids and a high surface area advantageous for pronounced protein adsorption. The nanotubes were coated easily on the fiber surface using an electrophoretic method. In a conventional contact angle test, a drop of water rapidly penetrated into the nanotube-coated fibermat. The culture test using murine osteoblast-like cells (MC3T3-E1) showed that the cell attachment to the nanotube-coated fibermat at an early stage after seeding was enhanced in comparison with that to the noncoated one. This approach may provide a new method of improving the surface of polymer-based biomaterials.

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Electrospun microfiber meshes of silicon-doped vaterite/poly(lactic acid) hybrid for guided bone regeneration

Cited by 89 publications

References 34 publications

Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold

Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold

Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration

Aluminum Silicate Nanotube Coating of Siloxane‐Poly(lactic acid)‐Vaterite Composite Fibermats for Bone Regeneration

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