Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Zhao, Tianbao; Zhang, Jianhua; Gao, Xiaoyan; Yuan, Dandan; Gu, Zhipeng; Xu, Yuanting

doi:10.1039/d2tb01182d

Cited by 23 publications

(12 citation statements)

References 283 publications

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“…This effective concentration is in general agreement with the results of Huang et al 500μM metformin promoted proliferation and osteogenic differentiation of MC3T3-E1 cells (Huang et al, 2022). For bone regeneration, activating the selfrepair path that involves inhibition of inflammation, promoting osteogenesis, and creating a new vascular supply is essential (Zhao et al, 2022). The higher concentrations of metformin (1,000 μM) had some ability to induce osteogenic differentiation of cells.…”

Section: Discussionsupporting

confidence: 89%

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

Liu

Fu²,

Lv³

et al. 2022

Front. Bioeng. Biotechnol.

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This study aimed to examine the effects of loading different concentrations of metformin onto an α-hemihydrate calcium sulfate/nano-hydroxyapatite (α-CSH/nHA) composite. The material characteristics, biocompatibility, and bone formation were compared as functions of the metformin concentration. X-ray diffraction results indicated that the metformin loading had little influence on the phase composition of the composite. The hemolytic potential of the composite was found to be low, and a CCK-8 assay revealed only weak cytotoxicity. However, the metformin-loaded composite was found to enhance the osteogenic ability of MC3T3-E1 cells, as revealed by alkaline phosphate and alizarin red staining, real-time PCR, and western blotting, and the optimal amount was 500 µM. RNA sequencing results also showed that the composite material increased the expression of osteogenic-related genes. Cranial bone lacks muscle tissue, and the low blood supply leads to poor bone regeneration. As most mammalian cranial and maxillofacial bones are membranous and of similar embryonic origin, the rat cranial defect model has become an ideal animal model for in vivo experiments in bone tissue engineering. Thus, we introduced a rat cranial defect with a diameter of 5 mm as an experimental defect model. Micro-computed tomography, hematoxylin and eosin staining, Masson staining, and immunohistochemical staining were used to determine the effectiveness of the composite as a scaffold in a rat skull defect model. The composite material loaded with 500 µM of metformin had the strongest osteoinduction ability under these conditions. These results are promising for the development of new methods for repairing craniofacial bone defects.

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Section: Discussionsupporting

confidence: 89%

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

Liu

Fu²,

Lv³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Considering the mechanical stability and composition of the fiber scaffolds during the design process, the ECM of tissues with differing properties, such as skin or bones, can specifically be simulated. 23,26 Despite their implementation in tissue culture, electrospun scaffolds have only recently been described for the cultivation of bacterial biofilms. 27,28 Even though their particular suitability to simulate a native biofilm microenvironment is increasingly being acknowledged, to the best of our knowledge, biofilms cultivated on electrospun scaffolds have not yet been used for modeling tissue infections.…”

Section: Introductionmentioning

confidence: 99%

Imitating the microenvironment of native biofilms using nanofibrous scaffolds to emulate chronic wound infections

et al. 2023

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“…27 So far, various methods have been developed to fabricate scaffolds with 3D structures, including adding sacrificial components, wet electrospinning, gas foaming, dispersion-shaping, and 3D fabrication. 28 Additive manufacturing, also known as 3D printing, has made advances both in manufacturing and in regenerative medicine. 29–31 The ability to create custom structures with repeating and specific internal morphologies enables the development of scaffolds for the regeneration of tissues, such as skin, cartilage, or bone.…”

Section: Introductionmentioning

confidence: 99%

Additive manufacturing of barium-doped calcium silicate/poly-ε-caprolactone scaffolds to activate CaSR and AKT signalling and osteogenic differentiation of mesenchymal stem cells

et al. 2023

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Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Abstract: In recent years, a variety of novel materials and processing technologies have been developed to prepare tissue engineering scaffolds for bone defect repair. Among them, nanofibers fabricated via electrospinning technology...

Cited by 23 publications

References 283 publications

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

α-Hemihydrate calcium sulfate/n-hydroxyapatite combined with metformin promotes osteogenesis in vitro and in vivo

Imitating the microenvironment of native biofilms using nanofibrous scaffolds to emulate chronic wound infections

Additive manufacturing of barium-doped calcium silicate/poly-ε-caprolactone scaffolds to activate CaSR and AKT signalling and osteogenic differentiation of mesenchymal stem cells

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