Electrospun nanofibers immobilized with collagen for neural stem cells culture

Abstract

Fibrous mats via electrospinning have been widely applied in tissue engineering. In this work, nanofibers were prepared via electrospinning from polymer with different content of carboxyl groups. A natural material, collagen, was then immobilized onto the nanofiber surface by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)/N-Hydroxysuccinimide (NHS) activation process. It was found that the immobilization degree of collagen could be facilely modulated. The obtained collagen-modified nanofibers were used for neural stem cells culture, and unmodified nanofibers were used as a control. Results indicated that the modification of collagen could enhance the attachment and viability of the cultured neural stem cells.

Electrospun Nanofibrous Materials for Neural Tissue Engineering

“…Collagen type I was immobilized on electrospun poly-methyl methacrylate (PMMA) and acrylic acid (AA) (PMMAAA) nano-fibrous scaffolds. The amount of carboxyl content of the nanofibers correlated directly with the amount of collagen immobilization [38]. Cell viability and neurite extension of the cortical neurons increased with increasing collagen immobilization as a result of the carboxyl content [38].…”

“…The amount of carboxyl content of the nanofibers correlated directly with the amount of collagen immobilization [38]. Cell viability and neurite extension of the cortical neurons increased with increasing collagen immobilization as a result of the carboxyl content [38]. Electrospun PCL treated with ethylenediamine (ED) increased the hydrophilicity, thus, affecting cell attachment but did not affect the differentiation of rat brain-derived neural stem cells [39].…”

Electrospun fibers in regenerative tissue engineering and drug delivery

“…SC attachment and differentiation is tunable based on [18]. Functionalizing with collagen improves the stem cell attachment and viability [19]. Encapsulating neural growth factors (NGF), glial cell derived neutrophic factor in nanofibers, facilitates the guided nerve regeneration.…”

Tissue Engineering Strategies Designed to Realize the Endogenous Regenerative Potential of Peripheral Nerves

“…Therefore, to boost cell viability collagen immobilized nanofibers have been used to culture cortical neural stem cells and could be used for peripheral nerve scaffolds. [145] Thus, when cell transplantation techniques are combined with other previously discussed strategies, the endogenous mechanisms of nerve regeneration can be significantly enhanced.…”