Electrospun Nanofibers of Curdlan (β‐1,3 Glucan) Blend as a Potential Skin Scaffold Material

Abstract

Curdlan (β‐1,3 glucan) (7 wt%) with polyvinyl alcohol (PVA) (10 wt%) is blended at 1:2 weight ratio and electrospun to get nanofibers and is crosslinked with glutaraldehyde vapor to make it insoluble in water. It has a fiber diameter of less than 100 nm and is hydrophilic (contact angle = 35°). It is biodegradable (10% in 14 d) and also has a good swelling behavior (≈170%). More than 100% of L6 cells are viable on this scaffold after 3 d. The scanning electron microscope images also reveal that cells are able to attach and spread in the nanofibrous scaffolds. In vitro scratch assay indicates that the wound closure rate of curdlan/PVA scaffold is better than PVA scaffold probably due to the immunomodulatory properties of the biopolymer. Thus our results indicate that curdlan/PVA scaffold can be an ideal material for wound healing applications.

Silver Loaded Nanofibrous Curdlan Mat for Diabetic Wound Healing: An In Vitro and In Vivo Study

“…Our previous report describes the fabrication and characterization of poly(vinyl alcohol) (PVA)/curdlan electrospun scaffold for wound healing application. [6] This study is focused on modification of PVA/curdlan electrospun scaffolds by the addition of silver in order to impart antibacterial property to eliminate infection in diabetic wound. Silver nitrate (AgNO 3 ) is used as a precursor to obtain silver nanoparticles while formic acid, the solvent for curdlan, acts as the reducing agent.…”

“…A bacterially derived polymer, curdlan is chosen here to produce a nanofibrous mat as a diabetic wound dressing material as it is known to possess anti‐inflammatory, anti‐infective, and wound healing properties. Our previous report describes the fabrication and characterization of poly(vinyl alcohol) (PVA)/curdlan electrospun scaffold for wound healing application . This study is focused on modification of PVA/curdlan electrospun scaffolds by the addition of silver in order to impart antibacterial property to eliminate infection in diabetic wound.…”

Nanofibers for Biomedical and Healthcare Applications

“…Electrospun nanofiber can be fabricated from a wide range of materials with high similarity to the native extracellular matrix in different sizes and functions. [108] Electrospun scaffolds have been employed in a number of different tissue applications including: vasculature, [109][110][111][112] skin, [113,114] bone, [115][116][117][118][119][120][121] cartilage, [122,123] neural, [124,125] and tendon/ligament ( Table 1). [126][127][128] Scaffolds with 3D structure can be synthesized from natural polymers, synthetic polymers, or blends of synthetic and natural polymers.…”

“…Condrocyte [123] Skin Poly(ε-caprolactone) (PCL) nanofibres with bioactive glass NPs Human skin fibroblast cells (HSFs) [159] Polyvinyl alcohol (PVA)/gum tragacanth/poly(ε-caprolactone) (PCL) hybrid NIH 3T3 fibroblast cell [160] Curdlan (β-1,3 glucan) (7 wt%) with polyvinyl alcohol (PVA) (10 wt%) L6 cells [113] Blend of poly-d,l-lactide (PDLLA) and poly(ethylene glycol) (PEG) Human dermal fibroblasts (HDFs) [173] Poly[lactic acid-co-glycolic acid] (PLAGA) Human skin fibroblasts (hSF) [172] Blends of chitosan, gelatin, and poly(ε-caprolactone) (PCL) Human foetal fibroblasts (cell line HFFF2) [173] Ju et al [155] fabricated PCL/collagen bilayer scaffold with desired mechanical properties by controlling nanofiber diameter. Enhancing the scaffold's porosity reduced its Young's modulus from 2.03 to 0.26 MPa by increasing the fiber diameter from 0.27 to 4.45 µm.…”

Regeneración Del Órgano Cutáneo Mediante Ingeniería De Tejidos

“…La prueba de rasguño in vitro indica que la tasa de cierre de la herida del andamio Curdlan/PVA es mejor que el andamio PVA, probablemente debido a las propiedades inmunomoduladoras del biopolímero. Los resultados indican que el andamio Curdlan/PVA puede ser un material ideal para las aplicaciones curativas de heridas [37].…”