Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

Abstract

In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications.

Eco-Friendly Hybrid PLLA/Chitosan/Trichoderma asperellum Nanomaterials as Biocontrol Dressings against Esca Disease in Grapevines

“…Polymer, metal, ceramic and composite nanofibers became easily able to be fabricated [8]. Electrospun nanofibers possess high surface-to-volume ratios and high porosity [9], making them suitable candidates for use in a variety of applications, such as tissue engineering [10], wound dressing [11], drug delivery [12], filtration [13], composite reinforcement [14], protective clothing [15], smart textiles [16], batteries [17], capacitors [18], sensors [19], catalysts [20], etc. ; however, the use of electrospun nanofibers in agriculture is relatively new [21].…”

The Possibility of Reuse of Nanofiber Mats by Machine Washing at Different Temperatures

“…Electrospun nanofiber membranes are used in protective masks and provide physical barriers to particles and viruses through smaller fiber diameters and pore diameters; they remove the restriction of static electricity, in contrast to surgical masks [7]. Electrospinning technology provides an easy way to produce nanofiber mats [8,9] for various purposes, from a wide range of biobased and synthetic polymers [10][11][12] and admixtures of particles [13,14], etc. Using electrospinning technology, nanofiber mats can be produced easily and cost-effectively for use in electronics [15], battery storage [13,16], medical and biotech-nology [17], water and air filtration [18], protective clothing [19,20], tissue engineering [21] and other applications.…”

Zingiber cassumunar Roxb. Essential Oil-Loaded Electrospun Poly(lactic acid)/Poly(ethylene oxide) Fiber Blend Membrane for Antibacterial Wound Dressing Application

“…These extraordinary characteristics exert several advantages for wound healing, such as assisting cell attachment and proliferation as well as facilitating the permeability of moisture and gas, which are beneficial for cell growth and allowing the absorption of additional exudates containing nutrients for bacterial growth. In addition, nanofiber scaffolds closely mimic the structure of the extracellular matrix (ECM) [15][16][17]. Therefore, nanofibers are promising candidates for wound dressing materials as they provide a suitable environment for wound healing.…”