Electrospun Polycaprolactone 3D Nanofibrous Scaffold with Interconnected and Hierarchically Structured Pores for Bone Tissue Engineering

Xu, Tao; Miszuk, Jacob M.; Zhao, Yong; Sun, Hongli; Fong, Hao

doi:10.1002/adhm.201500345

Cited by 253 publications

(207 citation statements)

References 45 publications

Supporting

Mentioning

199

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the high tunability and low cost enable gelatin to be a versatile polymer for biomedical applications. It has been recognized that nanofibrous scaffolds with connected macropore structure that mimic the natural ECM are favorable for osteoblastic differentiation and bone formation [50–52]. Although our data confirmed that the GF scaffolds can non-specifically absorb a small amount of rhBMP2, the affinity of BMPs to gelatin scaffolds is still limited due to the absence of the BMP-binding moieties.…”

Section: Discussionmentioning

confidence: 43%

BBP-functionalized biomimetic nanofibrous scaffolds can capture BMP2 and promote osteogenic differentiation

et al. 2017

Self Cite

View full text Add to dashboard Cite

Bone morphogenetic proteins (BMPs, e.g., BMP2 and 7) are potent mediators for bone repair, however, their clinical use has been limited by their safety and cost-effectiveness. Therefore, innovative strategies that can improve the efficacy of BMPs, and thereby, use a lower dose of exogenous BMPs are highly desired. Inspired by the natural interaction between extracellular matrix (ECM) and growth factors, we hypothesize that bone matrix-mimicking nanofibrous scaffold functionalized with BMP binding moieties can selectively capture and stabilize BMPs, and thereby, promote BMP-induced osteogenic differentiation. To test our hypothesis, a gelatin nanofibrous scaffold was fabricated using thermally induced phase separation together with a porogen leaching technique (TIPS&P) and functionalized by a BMP-binding peptide (BBP) through cross-linking. Our data indicated that BBP decoration largely improved the BMP2 binding and retention capacity of the nanofibrous scaffolds without compromising their macro/microstructure and mechanical properties. Importantly, the BBP-functionalized gelatin scaffolds were able to significantly promote BMP2-induced osteogenic differentiation. Moreover, BBP alone was able to significantly stimulate endogenous BMP2 expression and improve osteogenic differentiation. Compared to other affinity-based drug delivery strategies, e.g., heparin and antibody-mediated growth factor delivering techniques, we expect BBP-functionalized scaffolds will be a safer, more feasible and selective strategy for endogenous BMP stimulating and binding. Therefore, our data suggests a promising application of using the BBP-decorated gelatin nanofibrous scaffold to stimulate/capture BMPs and promote endogenous bone formation in situ in contrast to relying on the administration of high doses of exogenous BMPs and transplantation of cells.

show abstract

Section: Discussionmentioning

confidence: 43%

BBP-functionalized biomimetic nanofibrous scaffolds can capture BMP2 and promote osteogenic differentiation

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…In our recently published paper [22], a new strategy of thermally induced (nanofiber) self-agglomeration (TISA) followed by freeze drying has been reported; in specific, as-electrospun polycaprolactone (PCL) nanofibrous mats can first be converted into short individual nanofibers and tiny nanofibrous pieces, which can subsequently be utilized as building materials for making 3D electrospun PCL nanofibrous scaffolds. The resulting 3D scaffolds have the porosity of ~96% and possess the interconnected and hierarchically structured pores including macropores with sizes up to 300 µm.…”

Section: Introductionmentioning

confidence: 99%

“…Our in vitro cell differentiation results indicated that 3D electrospun PCL nanofibrous scaffolds (prepared via the TISA technique) were more favorable for chondrogenic (rather than osteogenic) differentiation, probably attributed to the relatively low stiffness/modulus of PCL [22]. Although these scaffolds could support bone morphogenetic protein 2 (BMP2) induced ectopic endochondral bone formation, new bones were identified merely in the surrounding areas but not inside the implanted scaffolds, suggesting that these scaffolds might need improvements to encourage/facilitate the bone formation.…”

Section: Introductionmentioning

confidence: 99%

Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation

et al. 2017

Self Cite

View full text Add to dashboard Cite

Nanofibrous scaffolds that are morphologically/structurally similar to natural ECM are highly interested for tissue engineering; however, the electrospinning technique has the difficulty in directly producing clinically relevant 3D nanofibrous scaffolds with desired structural properties. To address this challenge, we have developed an innovative technique of thermally induced nanofiber self-agglomeration (TISA) recently. The aim of this work was to prepare (via the TISA technique) and evaluate 3D electrospun PCL/PLA blend (mass ratio: 4/1) nanofibrous scaffolds having high porosity of ~95.8% as well as interconnected and hierarchically structured pores with sizes from sub-micrometers to ~300 µm for bone tissue engineering. The hypothesis was that the incorporation of PLA (with higher mechanical stiffness/modulus and bioactivity) into PCL nanofibers would significantly improve human mesenchymal stem cells (hMSCs) osteogenic differentiation in vitro and bone formation in vivo. Compared to neat PCL-3D scaffolds, PCL/PLA-3D blend scaffolds had higher mechanical properties and in vitro bioactivity; as a result, they not only enhanced the cell viability of hMSCs but also promoted the osteogenic differentiation. Furthermore, our in vivo studies revealed that PCL/PLA-3D scaffolds considerably facilitated new bone formation in a critical-sized cranial bone defect mouse model. In summary, both in vitro and in vivo results indicated that novel 3D electrospun PCL/PLA blend nanofibrous scaffolds would be strongly favorable/desired for hMSCs osteogenic differentiation and cranial bone formation.

show abstract

“…Electrospun PCL 3D nanofibrous with soft and highly porous scaffolds was developed in a study. In vivo studies indicated that this scaffold acts as an extracellular matrix for the regeneration of bone via a physiological endochondral ossification process (25).…”

Section: Poly(α-hydroxy Acids)mentioning

confidence: 99%

Biodegradable Polymers: Medical Applications

Kunduru

Basu

Domb

2016

Encyclopedia of Polymer Science and Technology

View full text Add to dashboard Cite

Biodegradable polymers are the “green materials” of the future. They break down into smaller chemical fragments and are finally eliminated from the body. Biodegradable polymers have been used for more than 50 years with diverse applications such as surgical sutures, wound dressings, tissue regeneration, enzyme immobilization, controlled drug delivery and gene delivery, tissue engineering scaffold, cryopreservation, nanotechnology, medical implants and devices, prosthetics, augmentation, cosmetics, sanitation products, coatings, adhesives, and many more. This article reviews synthetic (esters, amides, ethers, urethanes) and natural (polysaccharides and proteins) biodegradable polymers, highlighting their biomedical applications.

show abstract

Electrospun Polycaprolactone 3D Nanofibrous Scaffold with Interconnected and Hierarchically Structured Pores for Bone Tissue Engineering

Cited by 253 publications

References 45 publications

BBP-functionalized biomimetic nanofibrous scaffolds can capture BMP2 and promote osteogenic differentiation

BBP-functionalized biomimetic nanofibrous scaffolds can capture BMP2 and promote osteogenic differentiation

Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation

Biodegradable Polymers: Medical Applications

Contact Info

Product

Resources

About