Electrospun Polycaprolactone Nanofibers: Current Research and Applications in Biomedical Application

Azari, Arezo; Golchin, Ali; Maymand, Maryam Mahmoodinia; Mansouri, Fatemeh; Ardeshirylajimi, Abdolreza

doi:10.34172/apb.2022.070

Cited by 31 publications

(22 citation statements)

References 102 publications

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“…We developed fluorescent pH-sensing nanofiber scaffolds composed of optical pH sensor microparticles, highly suitable for monitoring pH changes in the surrounding environment, − and polycaprolactone (PCL) nanofibers, because of their processability, biocompatibility, biodegradability, and mechanical stability. − The morphology of the hybrid pH-sensing fibers was studied in detail by means of scanning electron microscopy (SEM) and CLSM. The SEM images in Figure a,b show random nonwoven mat of PCL nanofibers bearing spherical pH sensors aligned along the fiber longitudinal axis.…”

Section: Resultsmentioning

confidence: 99%

Probing Single-Cell Fermentation Fluxes and Exchange Networks via pH-Sensing Hybrid Nanofibers

et al. 2022

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The homeostatic control of their environment is an essential task of living cells. It has been hypothesized that, when microenvironmental pH inhomogeneities are induced by high cellular metabolic activity, diffusing protons act as signaling molecules, driving the establishment of exchange networks sustained by the cell-to-cell shuttling of overflow products such as lactate. Despite their fundamental role, the extent and dynamics of such networks is largely unknown due to the lack of methods in single-cell flux analysis. In this study, we provide direct experimental characterization of such exchange networks. We devise a method to quantify single-cell fermentation fluxes over time by integrating high-resolution pH microenvironment sensing via ratiometric nanofibers with constraint-based inverse modeling. We apply our method to cell cultures with mixed populations of cancer cells and fibroblasts. We find that the proton trafficking underlying bulk acidification is strongly heterogeneous, with maximal single-cell fluxes exceeding typical values by up to 3 orders of magnitude. In addition, a crossover in time from a networked phase sustained by densely connected "hubs" (corresponding to cells with high activity) to a sparse phase dominated by isolated dipolar motifs (i.e., by pairwise cell-to-cell exchanges) is uncovered, which parallels the time course of bulk acidification. Our method addresses issues ranging from the homeostatic function of proton exchange to the metabolic coupling of cells with different energetic demands, allowing for real-time noninvasive singlecell metabolic flux analysis.

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Section: Resultsmentioning

confidence: 99%

Probing Single-Cell Fermentation Fluxes and Exchange Networks via pH-Sensing Hybrid Nanofibers

et al. 2022

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“…We developed fluorescent pH-sensing nanofiber scaffolds composed of optical pH-sensors microparticles, highly suitable for monitoring pH changes in the surrounding environment [28–35], and polycaprolactone (PCL) nanofibers, because of their processability, biocompatibility, biodegradability, and mechanical stability [36–41].…”

Section: Resultsmentioning

confidence: 99%

“…We developed fluorescent pH-sensing nanofiber scaffolds composed of optical pHsensors microparticles, highly suitable for monitoring pH changes in the surrounding environment [28][29][30][31][32][33][34][35], and polycaprolactone (PCL) nanofibers, because of their processability, biocompatibility, biodegradability, and mechanical stability [36][37][38][39][40][41]. The morphology of the hybrid pH-sensing fibers was studied in detail by means of scanning electron microscopy (SEM) and CLSM.…”

Section: Fabrication Of Ph-sensing Scaffoldsmentioning

confidence: 99%

Probing single cell fermentation flux and intercellular exchange networks via pH-microenvironment sensing and inverse modeling

Onesto

Forciniti

Alemanno

et al. 2022

Preprint

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The homeostatic control of their environment is an essential task of living cells. It has been hypothesized that when microenvironmental pH inhomogeneities are induced by high cellular metabolic activity, diffusing protons act as signaling molecules, driving the establishment of cross-feeding networks sustained by the cell-to-cell shuttling of overflow products such as lactate. Despite their fundamental role, the extent and dynamics of such networks is largely unknown due to the lack of methods in single cell flux analysis. In this study we provide direct experimental characterization of such exchange networks. We devise a method to quantify single cell fermentation fluxes over time by integrating high-resolution pH microenvironment sensing via ratiometric nanofibers with constraint-based inverse modeling. We apply our method to cell cultures with mixed populations of cancer cells and fibroblasts. We find that the proton trafficking underlying bulk acidification is strongly heterogeneous, with maximal single cell fluxes exceeding typical values by up to 3 orders of magnitude. In addition, a crossover in time from a networked phase sustained by densely connected “hubs” (corresponding to cells with high activity) to a sparse phase dominated by isolated dipolar motifs (i.e. by pair-wise cell-to-cell exchanges) is uncovered, which parallels the time course of bulk acidification. Our method promises to shed light on issues ranging from the homeostatic function of proton exchange to the metabolic coupling of cells with different energetic demands, and paves the way for real-time non-invasive single cell metabolic flux analysis.

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“…Combining biological and mechanical properties of PCL with drug delivery capacity of PEG as a biocompatible lubricant agent were studied in our previous report in which PCL: PEG: Aloe-Vera nanofibers had been fabricated and evaluated for bone repairing application 13 . According to the other reports, the properties of PCL and PEG have turned them in to frequently used raw materials in development of several burn wound care dressings which have succeeded in receiving FDA-approval 14 , 15 .…”

Section: Introductionmentioning

confidence: 99%

Preclinical evaluation of the polycaprolactone-polyethylene glycol electrospun nanofibers containing egg-yolk oil for acceleration of full thickness burns healing

Shadman-Manesh

Gholipour-Kanani

Najmoddin

et al. 2023

Sci Rep

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Considering the great potential of egg yolk oil (EYO) in management of burn wounds and superb biological properties of polycaprolactone (PCL) and polyethylene glycol (PEG), hereby, a PCL-PEG-EYO scaffold was developed by electrospinning method for burn healing. The physico-chemical characterizations were performed using SEM, FTIR and contact angle tests. The biological properties of the fabricated scaffolds were evaluated by antibacterial test, in vitro cell culturing, MTT assay and in vivo experiments. The SEM images of PCL-PEG-EYO nanofibers demonstrated a uniform bead-free morphology with 191 ± 61 nm diameter. The fabricated scaffold revealed hydrophilicity with the water contact angel of 77°. No cytotoxicity was observed up to 7 days after cell culturing onto the PCL-PEG-EYO nanofibrous surface. The presence of EYO in the PCL-PEG-EYO scaffold meaningfully improved the cell viability, proliferation and attachment compared to PCL-PEG scaffold. Moreover, the PCL-PEG-EYO scaffolds demonstrated antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa bacteria strain. Finally, a statistically significant enhancement in wound closure, re-epithelialization, angiogenesis and collagen synthesis was observed at the end of 21-day treatment period using PCL-PEG-EYO nanofibrous scaffold. Overall, the PCL-PEG-EYO nanofibrous scaffolds demonstrated a great potential in management of full thickness burn wounds in vivo.

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Electrospun Polycaprolactone Nanofibers: Current Research and Applications in Biomedical Application

Cited by 31 publications

References 102 publications

Probing Single-Cell Fermentation Fluxes and Exchange Networks via pH-Sensing Hybrid Nanofibers

Probing Single-Cell Fermentation Fluxes and Exchange Networks via pH-Sensing Hybrid Nanofibers

Probing single cell fermentation flux and intercellular exchange networks via pH-microenvironment sensing and inverse modeling

Preclinical evaluation of the polycaprolactone-polyethylene glycol electrospun nanofibers containing egg-yolk oil for acceleration of full thickness burns healing

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