Electrospun polystyrene–poly(styrene-co-maleic anhydride) nanofiber as a new aptasensor platform

Lee, Su Jin; Tatavarty, Rameshwar; Gu, Man Bock

doi:10.1016/j.bios.2012.06.009

Cited by 45 publications

(24 citation statements)

References 36 publications

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“…These latter studies can be grouped on the basis of aptamers associated to quantum dots (QDs) [142,198], nanoparticles [195,199,200], carbon nanotubes [201], or label-free [202] as compared with antibodiesbased studies [203]. Lee et al (2012) [204] were the only ones, to the best of our knowledge, who described the use of an aptamer-immobilised electrospun polystyrene-poly(styrene-co-maleicanhydride) (PS-PSMA) nanofibre as a new cost-effective aptasensor platform for protein detection (Fig. 10.11).…”

Section: Aptamers Biosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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Section: Aptamers Biosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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“…These latter studies can be grouped on the basis of aptamers associated to quantum dots (QDs) [142,198], nanoparticles [195,199,200], carbon nanotubes [201], or label-free [202] as compared with antibodiesbased studies [203]. [204] were the only ones, to the best of our knowledge, who described the use of an aptamer-immobilised electrospun polystyrene-poly(styrene-co-maleicanhydride) (PS-PSMA) nanofibre as a new cost-effective aptasensor platform for protein detection (Fig. 10.11).…”

Section: Aptamers Biosensorsmentioning

confidence: 99%

“…The fibres functionalisation occurred by incubation with streptavidin in PBS buffer solution for 1 h at room temperature and could be stored at 4 C. Streptavidin-coated nanofibres, after washing, were immersed in 1 nmol biotin-aptamer B&W buffer solution (Tris-HCl, EDTA, Optical signals (bottom) of thrombin binding assays using human serum samples. (a) Fluorescence spectra obtained from concentration-response assays using aptamers-on-nanofibre biosensors with Q-TBA15 as the signaling probe and (b) relative fluorescence signal intensities (F À F0) of thrombin detection [204] (Copyright ©2012 Elsevier B.V.) NaCl, pH 7.5) under mild rotation at RT for 1 h to allow the aptamer immobilisation. Such a scaffold worked as an excellent capturing fluorescent probe for the thrombin detection.…”

Section: Aptamers Biosensorsmentioning

confidence: 99%

Electrospinning for High Performance Sensors

Macagnano¹,

Zampetti²,

Kny³

2015

NanoScience and Technology

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The series NanoScience and Technology is focused on the fascinating nano-world, mesoscopic physics, analysis with atomic resolution, nano and quantum-effect devices, nanomechanics and atomic-scale processes. All the basic aspects and technology-oriented developments in this emerging discipline are covered by comprehensive and timely books. The series constitutes a survey of the relevant special topics, which are presented by leading experts in the field. These books will appeal to researchers, engineers, and advanced students.More information about this series at

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“…Compared to other enzyme nanocarriers, polymer-based materials can be easily produced in large quantities, and importantly the polymer nanocarries can provide a number of reactive groups for fabrication of NBC assemblies with high stability and functional activities [26,67,68]. Polymers have been widely used as enzyme supports.…”

Section: Polymer Nanocarriersmentioning

confidence: 99%

“…Copolymer NFs have been prepared from maleic anhydride and styrene as a new aptasensor platform [67]. The abundance of maleic anhydride promoted high loading of aptamers to ensure sufficient capacity to capture enzymes.…”

Section: Polymer Nanocarriersmentioning

confidence: 99%

Nanobiocatalyst advancements and bioprocessing applications

Misson

Zhang

Jin

2015

J. R. Soc. Interface.

214

108

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The nanobiocatalyst (NBC) is an emerging innovation that synergistically integrates advanced nanotechnology with biotechnology and promises exciting advantages for improving enzyme activity, stability, capability and engineering performances in bioprocessing applications. NBCs are fabricated by immobilizing enzymes with functional nanomaterials as enzyme carriers or containers. In this paper, we review the recent developments of novel nanocarriers/nanocontainers with advanced hierarchical porous structures for retaining enzymes, such as nanofibres (NFs), mesoporous nanocarriers and nanocages. Strategies for immobilizing enzymes onto nanocarriers made from polymers, silicas, carbons and metals by physical adsorption, covalent binding, cross-linking or specific ligand spacers are discussed. The resulting NBCs are critically evaluated in terms of their bioprocessing performances. Excellent performances are demonstrated through enhanced NBC catalytic activity and stability due to conformational changes upon immobilization and localized nanoenvironments, and NBC reutilization by assembling magnetic nanoparticles into NBCs to defray the high operational costs associated with enzyme production and nanocarrier synthesis. We also highlight several challenges associated with the NBC-driven bioprocess applications, including the maturation of large-scale nanocarrier synthesis, design and development of bioreactors to accommodate NBCs, and long-term operations of NBCs. We suggest these challenges are to be addressed through joint collaboration of chemists, engineers and material scientists. Finally, we have demonstrated the great potential of NBCs in manufacturing bioprocesses in the near future through successful laboratory trials of NBCs in carbohydrate hydrolysis, biofuel production and biotransformation.

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Electrospun polystyrene–poly(styrene-co-maleic anhydride) nanofiber as a new aptasensor platform

Cited by 45 publications

References 36 publications

Electrospinning-Based Nanobiosensors

Electrospinning-Based Nanobiosensors

Electrospinning for High Performance Sensors

Nanobiocatalyst advancements and bioprocessing applications

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