Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications

Chandika, Pathum; Oh, Gun‐Woo; Heo, Seong‐Yeong; Kim, Se-Chang; Kim, Tae-Hee; Kim, Min-Sung; Jung, Won‐Kyo

doi:10.1016/j.msec.2021.111871

Cited by 64 publications

(38 citation statements)

References 51 publications

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“…Moreover, our newly developed dressing also slightly improved granulation tissue formation and this effect persisted when NANO was loaded with LPPO. The healing promoting effect can even be improved by specific modifications of the material structure, for example by combining the PCL with fish collagen and covalently bonded chitooligosaccharides 38 . Another scaffold comprised two supportive PCL-chitosan layers (accelerating healing) on the sides and a polyvinyl alcohol-metformin hydrochloride (down-regulating expression of fibrosis-related genes) in the middle 39 .…”

Section: Discussionmentioning

confidence: 99%

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Pham

Jenčová

Kaňuchová

et al. 2021

Sci Rep

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Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of wound dressings based on the polycaprolactone nanofiber scaffold (NANO) releasing second generation lipophosphonoxin (LPPO) as antibacterial agent. Firstly, we demonstrated in vitro that LPPO released from NANO exerted antibacterial activity while not impairing proliferation/differentiation of fibroblasts and keratinocytes. Secondly, using a mouse model we showed that NANO loaded with LPPO significantly reduced the Staphylococcus aureus counts in infected wounds as evaluated 7 days post-surgery. Furthermore, the rate of degradation and subsequent LPPO release in infected wounds was also facilitated by lytic enzymes secreted by inoculated bacteria. Finally, LPPO displayed negligible to no systemic absorption. In conclusion, the composite antibacterial NANO-LPPO-based dressing reduces the bacterial load and promotes skin repair, with the potential to treat wounds in clinical settings.

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Section: Discussionmentioning

confidence: 99%

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Pham

Jenčová

Kaňuchová

et al. 2021

Sci Rep

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“…The hybridization of biocomposites material is the key to designing new components with good strength at a relatively lower cost [ 110 ]. The mechanical properties of PCL hybrid biocomposites gives an advantage of good strength at a lower cost, which can be used for applications that were not possible by using the green biocomposites [ 144 , 145 , 146 ].…”

Section: Polycaprolactone-based Hydrid Biocompositesmentioning

confidence: 99%

Natural Fiber-Reinforced Polycaprolactone Green and Hybrid Biocomposites for Various Advanced Applications

et al. 2022

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Recent developments within the topic of biomaterials has taken hold of researchers due to the mounting concern of current environmental pollution as well as scarcity resources. Amongst all compatible biomaterials, polycaprolactone (PCL) is deemed to be a great potential biomaterial, especially to the tissue engineering sector, due to its advantages, including its biocompatibility and low bioactivity exhibition. The commercialization of PCL is deemed as infant technology despite of all its advantages. This contributed to the disadvantages of PCL, including expensive, toxic, and complex. Therefore, the shift towards the utilization of PCL as an alternative biomaterial in the development of biocomposites has been exponentially increased in recent years. PCL-based biocomposites are unique and versatile technology equipped with several importance features. In addition, the understanding on the properties of PCL and its blend is vital as it is influenced by the application of biocomposites. The superior characteristics of PCL-based green and hybrid biocomposites has expanded their applications, such as in the biomedical field, as well as in tissue engineering and medical implants. Thus, this review is aimed to critically discuss the characteristics of PCL-based biocomposites, which cover each mechanical and thermal properties and their importance towards several applications. The emergence of nanomaterials as reinforcement agent in PCL-based biocomposites was also a tackled issue within this review. On the whole, recent developments of PCL as a potential biomaterial in recent applications is reviewed.

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“…In a recent study, novel bilayer electrospun nanofibrous scaffolds composed of fish collagen and PCL at various ratios were fabricated and covalently crosslinked with chitin oligosaccharides. The produced fiber mats induced the adhesion, infiltration, and proliferation of fibroblast (NHDF-neo) and keratinocyte (HaCaT) cells in vitro, exhibiting rapid healing when implanted on a full-thickness wound in vivo, confirming their potential as skin implants for tissue regeneration applications ( Figure 9 A) [ 235 ].…”

Section: Marine Animal-derived Biopolymersmentioning

confidence: 98%

“…( e ) Images of the in vivo wound healing study on mice treated with the fish collagen/PCL (9:1) electrospun nanofibers crosslinked with chitin oligosaccharides on days 0, 7, and 21. Reprinted/adapted with permission from [ 235 ], Copyright 2021, Elsevier. ( B ) SEM images of fish gelatin electrospun nanofibers ( a ) before and after crosslinking ( b ) via dehydrothermal treatment, ( c ) with glutaraldehyde for 5 h, and ( d ) after genipin bath.…”

Section: Marine Animal-derived Biopolymersmentioning

confidence: 99%

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

et al. 2022

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Marine biopolymers, abundantly present in seaweeds and marine animals, feature diverse structures and functionalities, and possess a wide range of beneficial biological activities. Characterized by high biocompatibility and biodegradability, as well as unique physicochemical properties, marine biopolymers are attracting a constantly increasing interest for the development of advanced systems for applications in the biomedical field. The development of electrospinning offers an innovative technological platform for the production of nonwoven nanofibrous scaffolds with increased surface area, high encapsulation efficacy, intrinsic interconnectivity, and structural analogy to the natural extracellular matrix. Marine biopolymer-based electrospun nanofibrous scaffolds with multifunctional characteristics and tunable mechanical properties now attract significant attention for biomedical applications, such as tissue engineering, drug delivery, and wound healing. The present review, covering the literature up to the end of 2021, highlights the advancements in the development of marine biopolymer-based electrospun nanofibers for their utilization as cell proliferation scaffolds, bioadhesives, release modifiers, and wound dressings.

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Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications

Cited by 64 publications

References 51 publications

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Natural Fiber-Reinforced Polycaprolactone Green and Hybrid Biocomposites for Various Advanced Applications

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

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