Electrospun Sandwich‐Structure Composite Membranes for Wound Dressing Scaffolds with High Antioxidant and Antibacterial Activity

Abstract

In this study, the sandwich‐structured composite (SSC) membranes with well‐antibacterial and antioxidant properties, which have the promising application as wound dressing, are successfully fabricated by combining an electrospinning process. The SSC membranes are composed of three layers, including the bottom polyvinylidene fluoride fibrous layer, the middle curcumin/polylactic acid (PLA) microsphere layer, and the top enrofloxacin/PLA fibrous layer, respectively. The obtained SSC membranes are characterized in terms of morphology, component, and mechanical property using scanning electronic microscope, X‐ray diffractometer, Fourier transform infrared spectroscopy, and universal electronic testing machine, respectively. Moreover, in vitro drug release, antioxidant activity, antimicrobial activity, and biocompatibility of the SSC membranes are also evaluated. The results showed that the obtained composite membranes indeed possess the sandwich structure, where the middle microsphere layer is located between two fibrous surface layers. It is found that the drug‐loaded SSC membranes show excellent antioxidant activity against •OH and DPPH free radicals and antibacterial activity against Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae, Pseudomonas aeruginosa, and Candida albicans. The combination of electrospinning and electrospraying opens up a new way to fabricate a variety of composite membranes with a sandwich structure, which have promising potential application as wound dressing scaffolds.

Electrospun Polyvinylpyrrolidone-Gelatin and Cellulose Acetate Bi-Layer Scaffold Loaded with Gentamicin as Possible Wound Dressing

“…The scaffold physical properties could assist in cell migration and differentiation [ 33 ], according to previously reported results where the lower the contact angle of the material, the greater the hydrophilicity and the higher the biocompatibility [ 34 ]. Considering these results, the potential application of this material as a dressing for wounds exists, given that the layer that would be in direct contact with the tissue would be the PG+gen and the CA layer could fulfill the role as physical barrier to avoid colonization by microorganisms [ 35 ].…”

Biocompatible and Biodegradable Electrospun Nanofibrous Membranes Loaded with Grape Seed Extract for Wound Dressing Application

“…The advantageous properties related to nanoscale dimensions of electrospun nanofibers (NFs) provide a possibility to control/extend the delivery of bioactive molecules to the wound site as well as to improve cell adhesion and proliferation [16,17]. Such systems are capable of mimicking the extracellular matrix of the various biological tissues, which can improve the biological performance of these membranes [20][21][22][23][24].…”

Waterproof and Breathable Electrospun Nanofibrous Membranes

“…A higher MVTR would cause wound dehydration and generate a scab, while low WVTR may result in wound maceration and inflammation . In this regard, many researchers engineered bilayer wound dressing integrating W&B nanofibrous membranes with hydrophilic material to obtain optimal breathability . Yang et al .…”