Electrospun silk-BMP-2 scaffolds for bone tissue engineering

Li, Chunmei; Vepari, Charu; Jin, Hyoung‐Joon; Kim, Hyeon Joo; Kaplan, David L.

doi:10.1016/j.biomaterials.2006.01.022

Cited by 1,074 publications

(736 citation statements)

References 56 publications

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“…collagen, silk fibroin, and fibrinogen) and synthetic polymers (e.g. PGA, PLLA, PLGA, and PCL) have been processed into fine nonwoven fabrics for tissue engineering research [48,[50][51][52][53][54][55][56][57][58][59][60]. Various cells have been reported to attach, proliferate, and differentiate into or maintain their functional phenotypes on these electrospun nano-fibrous materials [48,54,58,59,61,62].…”

Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,212

819

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,212

819

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“…Degradation lifetimes of silk can be regulated by the extent of physical crosslinking for beta sheet formation, allowing control of degradation timeframes from weeks to years [21], an important property for sustained release applications. Silk also can be processed under aqueous and ambient conditions in water, thus allowing for the incorporation of labile biomolecules without loss of biological function or activity [22,23]. Further, the frequent use of silk sutures in brain and nerve tissue confirms the feasibility of implantation of silk biomaterials in the brain.…”

Section: Introductionmentioning

confidence: 98%

Silk polymer-based adenosine release: Therapeutic potential for epilepsy

et al. 2008

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Adenosine augmentation therapies (AAT) make rational use of the brain's own adenosine-based seizure control system and hold promise for the therapy of refractory epilepsy. In an effort to develop an AAT compatible with future clinical application, we developed a novel silk protein-based release system for adenosine. Adenosine releasing brain implants with target release doses of 0, 40, 200, and 1,000 ng adenosine/day were prepared by embedding adenosine-containing microspheres into nanofilm-coated silk fibroin scaffolds. In vitro, the respective polymers released 0, 33.4, 170.5, and 819.0 ng adenosine/day over 14 days. The therapeutic potential of the implants was validated in a dose-response study in the rat model of kindling epileptogenesis. Four days prior to the onset of kindling, adenosine-releasing polymers were implanted into the infrahippocampal cleft and progressive acquisition of kindled seizures was monitored over a total of 48 stimulations. We document a dose-dependent retardation of seizure acquisition. In recipients of polymers releasing 819 ng adenosine/day, kindling epileptogenesis was delayed by one week corresponding to 18 kindling stimulations. Histological analysis of brain samples confirmed correct location of implants and electrodes. We conclude that silk-based delivery of around 1,000 ng adenosine/day is a safe and efficient strategy to suppress seizures.

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“…All spectra showed features of different apatite precipitates. Carbonate was present in all samples [11,33] and phosphate bands were visible between 1200 and 900 cm À1 [34][35][36][37]. Cell-seeded scaffolds cultured in osteogenic medium displayed a characteristic peak for phosphate ions (PO 4 3À ) at 1038 cm À1 , indicating a higher amount of mineralized tissue formation (Fig.…”

Section: Ftirmentioning

confidence: 88%