Electrospun thermosensitive hydrogel scaffold for enhanced chondrogenesis of human mesenchymal stem cells

Brunelle, Alexander; Horner, Christopher B.; Low, Karen; Ico, Gerardo; Nam, Jin

doi:10.1016/j.actbio.2017.11.020

Cited by 59 publications

(47 citation statements)

References 51 publications

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“…Scaffold microstructure (surface and cross-section) is commonly evaluated by SEM, AFM and light microscope like thermoresponsive macrogels [102][103][104][106][107][108]. Pore diameter and distribution or fiber size and alignment are often analyzed by randomly picking at least dozens of various segments by means of image J software [65,109].…”

Section: Morphology and Structurementioning

confidence: 99%

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

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Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote "on-off" behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by surface wettability changes and volume phase transitions of PNIPAAm modified substrates with respect to ambient temperature. The thermoresponsive culture platforms and matching thermal-liftoff method can effectively substitute for the traditional cell harvesting ways like enzymatic hydrolysis and mechanical scraping, and will improve the stable and high quality of recovered cells. Therefore, the establishment and detection on PNIPAAm based culture systems are of particular importance. This review covers the important developments and recommendations for future work of the preparation and characterization of temperature-responsive substrates based on PNIPAAm and analogues for cell culture applications.

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Section: Morphology and Structurementioning

confidence: 99%

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

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“…[112] As an attempt to overcome this drawback, biodegradable methacrylated thermogelling macromers (MA-TGMs) have been proposed. [126] After cell seeding and incubation at 37 °C, the thermosensitive hydrogel component of the hybrid scaffold quickly dissolved and filled the pores within the fiber structure. These macromers are particularly promising for cell encapsulation applications, as they facilitate biointegration and degradation via hydrolysis of the phosphate ester bonds of MAEP.…”

Section: Temperature-responsive Cell Encapsulation Systemsmentioning

confidence: 99%

“…In another study, pNIPAm was conjugated with poly(ethylene glycol) dimethacrylate (PEG-DMA) and incorporated within poly(ε-caprolactone) (PCL) electrospun fibers to construct a hybrid strategy for chondrogenesis of human MSCs. [126] After cell seeding and incubation at 37 °C, the thermosensitive hydrogel component of the hybrid scaffold quickly dissolved and filled the pores within the fiber structure. This led to a quick and mold-less single-step cell encapsulation strategy with superior mechanical properties compared to hydrogels alone.…”

Section: Temperature-responsive Cell Encapsulation Systemsmentioning

confidence: 99%

Design Principles and Multifunctionality in Cell Encapsulation Systems for Tissue Regeneration

Correia

Reis

Mano

2018

Adv Healthcare Materials

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Cell encapsulation systems are being increasingly applied as multifunctional strategies to regenerate tissues. Lessons afforded with encapsulation systems aiming to treat endocrine diseases seem to be highly valuable for the tissue engineering and regenerative medicine (TERM) systems of today, in which tissue regeneration and biomaterial integration are key components. Innumerous multifunctional systems for cell compartmentalization are being proposed to meet the specific needs required in the TERM field. Herein is reviewed the variable geometries proposed to produce cell encapsulation strategies toward tissue regeneration, including spherical and fiber-shaped systems, and other complex shapes and arrangements that better mimic the highly hierarchical organization of native tissues. The application of such principles in the TERM field brings new possibilities for the development of highly complex systems, which holds tremendous promise for tissue regeneration. The complex systems aim to recreate adequate environmental signals found in native tissue (in particular during the regenerative process) to control the cellular outcome, and conferring multifunctional properties, namely the incorporation of bioactive molecules and the ability to create smart and adaptative systems in response to different stimuli. The new multifunctional properties of such systems that are being employed to fulfill the requirements of the TERM field are also discussed.

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“…Thus, it is significant to deliver drugs to the target site directly so that such drugs can have the greatest pharmacological effect. Controlled drug delivery systems have been developed and used to overcome the defects of conventional formulations . However, even if some new materials have been discovered, the development of hydrophobic drug delivery systems is still in its infancy.…”

Section: Introductionmentioning

confidence: 99%

“…Controlled drug delivery systems have been developed and used to overcome the defects of conventional formulations. [1][2][3][4] However, even if some new materials have been discovered, the development of hydrophobic drug delivery systems is still in its infancy. For instance, baicalein, a potential cancer chemo-preventative compound of flavone, has been reported to inhibit cancer cell growth by inducing apoptosis and causing cell cycle arrest in various human cancer cell models.…”

Section: Introductionmentioning

confidence: 99%

Dual pH‐ and thermal‐responsive nanocomposite hydrogels for controllable delivery of hydrophobic drug baicalein

Feng

Wang

et al. 2019

Polymer International

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Hydrogels have been widely used as mild biomaterials due to their bio‐affinity, high drug loading capability and controllable release profiles. However, hydrogel‐based carriers are greatly limited for the delivery of hydrophobic payloads due to the lack of hydrophobic binding sites. Herein, nano‐liposome micelles were embedded in semi‐interpenetrating poly[(N‐isopropylacrylamide)‐co‐chitosan] (PNIPAAm‐co‐CS) and poly[(N‐isopropylacrylamide)‐co‐(sodium alginate)] (PNIPAAm‐co‐SA) hydrogels which were responsive to both temperature and pH, thereby establishing tunable nanocomposite hydrogel delivery systems. Nano‐micelles formed via the self‐assembly of phospholipid could serve as the link between hydrophobic drug and hydrophilic hydrogel due to their special amphiphilic structure. The results of transmission and scanning electron microscopies and infrared spectroscopy showed that the porous hydrogels were successfully fabricated and the liposomes encapsulated with baicalein could be well contained in the network. In addition, the experimental results of response release in vitro revealed that the smart hydrogels showed different degree of sensitiveness under different pH and temperature stimuli. The results of the study demonstrate that combining PNIPAAm‐co‐SA and PNIPAAm‐co‐CS hydrogels with liposomes encapsulated with hydrophobic drugs is a feasible method for hydrophobic drug delivery and have potential application prospects in the medical field. © 2018 Society of Chemical Industry

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Electrospun thermosensitive hydrogel scaffold for enhanced chondrogenesis of human mesenchymal stem cells

Cited by 59 publications

References 51 publications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Design Principles and Multifunctionality in Cell Encapsulation Systems for Tissue Regeneration

Dual pH‐ and thermal‐responsive nanocomposite hydrogels for controllable delivery of hydrophobic drug baicalein

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