Electrostatic effects on the electrical tension-induced irreversible pore formation in giant unilamellar vesicles

Karal, Mohammad Abu Sayem; Orchi, Urbi Shyamolima; Towhiduzzaman, Md.; Ahamed, Md. Kabir; Ahmed, Marzuk; Ahammed, Shareef; Mokta, Nadia Akter; Sharmin, Sabrina; Sarkar, Malay Kumar

doi:10.1016/j.chemphyslip.2020.104935

Cited by 14 publications

(14 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, with increasing electrostatic effects (increased surface charge density or decreased salt concentration), the probability of rupture increased. 36 To calculate the rate constant (k r ) of rupture, the timedependent fraction of intact GUVs (P intact (t)) out of all the examined GUVs was used. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The tting parameters of eqn (5) are A F and line tension (G). The electrostatic term B is dened as follows: 36,80…”

Section: Resultsmentioning

confidence: 99%

“…Recently, a technique has been developed for applying a constant electric tension to the membranes of GUVs using IRE, by which the electrodeformation, electrofusion, and rupture of charged and cholesterol-containing membranes have been investigated. 8,[35][36][37] In addition, the effect of osmotic pressure on the constant electric-tension-induced rupture of GUVs has also been studied. 11 Mechanical tension is applied to the membranes of GUVs using the micropipette aspiration technique, which helps in understanding the processes of pore formation and vesicle rupture and in estimating the elasticity of membranes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…The tting parameters of eqn (5) are A F and line tension (G). The electrostatic term B is dened as follows: 36,80…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Under osmotic pressure, the electric field induced a total tension, σ t = σ c + σ oseq , in the membranes of GUVs, where σ c is the tension due to electric field and σ oseq is the tension due to Π at swelling equilibrium. Using the mean first passage time approach the rate constant ( k p ) of tension-induced pore formation in the membranes of GUVs is determined as follows [ 55 ]: where A F is the pre-exponential factor, Γ is the line tension of membrane and B is the electrostatic interaction term due to charges of membrane. The theoretical Eq ( 3 ) was used to fit the experimental data on k p vs. σ t for both DOPG/DOPC/chol (46/39/15) and DOPG/DOPC (40/60)-GUVs.…”

Section: Methodsmentioning

confidence: 99%

Section: Rate Constant Of Tension-induced Pore Formation In Guvs Under Osmotic Pressurementioning

confidence: 99%

Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles

et al. 2021

Self Cite

View full text Add to dashboard Cite

Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC, with specific molar ratios. We determined the rate constant (kp) of rupture of DOPG/DOPC/chol (46/39/15)-GUVs and DOPG/DOPC (40/60)-GUVs induced by constant electric tension (σc) under different Π. The σc dependent kp values were fitted with a theoretical equation, and the corresponding membrane tension (σoseq) at swelling equilibrium under Π was estimated. The estimated membrane tension agreed well with the theoretical calculation within the experimental error. Interestingly, the values of σoseq were almost same for both types of synthesized GUVs under same osmotic pressure. We also examined the sucrose leakage, due to large osmotic pressure-induced pore formation, from the inside of DOPG/DOPC/chol(46/39/15)-GUVs. The estimated membrane tension due to large Π at which sucrose leaked out was very similar to the electric tension at which GUVs were ruptured without Π. We explained the σc and Π induced pore formation in the lipid membranes of GUVs.

show abstract

An investigation into the critical tension of electroporation in anionic lipid vesicles

et al. 2020

View full text Add to dashboard Cite

Electrostatic effects on the electrical tension-induced irreversible pore formation in giant unilamellar vesicles

Cited by 14 publications

References 48 publications

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

Recent developments in the kinetics of ruptures of giant vesicles under constant tension

Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles

An investigation into the critical tension of electroporation in anionic lipid vesicles

Contact Info

Product

Resources

About