Electrostatic effects upon adsorption and desorption of polylysines on the surface of lipid membranes of different composition

Finogenova, O.A.; Filinsky, D. V.; Ermakov, Yu. A.

doi:10.1134/s1990747808020128

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…But in the case of polypeptides their binding to the sur face is irreversible: the boundary potential does not return to its initial value. This property of large polyca tions of different nature was pointed out in our previ ous works [1,23,24]. Of special interest is the second slow phase in the kinetics of boundary potential change in the opposite direction to its initial fast change just after polylysine injection to the cell.…”

Section: Resultsmentioning

confidence: 60%

“…Polycations of different structure are known to adsorb at the negatively charged surfaces of cellular or model lipid membranes and significantly change the electric field distribution at their boundaries [1][2][3]. These effects are studied in many details for lysine based polypeptides that are widely applied to develop some medical drugs and to solve the various problems of biotechnology [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Structural factors of lysine and polylysine interaction with lipid membranes

Marukovich

Nesterenko

Ermakov

2015

Biochem. Moscow Suppl. Ser. A

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Electrostatic effects in lysine and polylysine adsorption at lipid membrane surface were studied. Electrokinetic measurements demonstrated that lysine induces similar dose dependent changes of zeta potential in suspensions of liposomes made from cardiolipin (CL) and phosphatidylserine (PS). These changes correlate well with numerical description of diffuse part of electric double layer by Gouy-Chap man-Stern model in assumption that both potassium cation and lysine molecules determine the ionic strength of the media. Good agreement with the electrokinetic data was found with the isotherm constructed for lysine distribution between bilayer and water with low constant (K d = 1.2 × 10 -3 M -1 ) independently of potassium adsorption (K = 1 M -1 ) on lipid molecules or for their competitive adsorption with the same con stants. Lysine adsorption induces total boundary potential changes of planar bilayer lipid membranes (BLM) from the same lipids registered by the method of intramembranous field compensation. In contrast to surface potential of liposomes in electrokinetic experiments the total boundary potential of BLM remains unchanged up to concentration of lysine that is about 1.5 orders of magnitude higher. This fact corresponds to changes in opposite directions of surface and dipole components of boundary potential. They compensate each other to some extent when lysine adsorbs at the surface. This explanation was supported by molecular dynamic sim ulation of bilayers from DOPS in the presence of lysine. According to the MD simulations, the compensation effect can be attributed to lysine effect on hydrogen bonds of water molecules with phosphate groups of lipids but not with carboxylic groups. A similar "compensation effect" was expected and observed with membranes from CL and PS. The amplitude of dipole effect was about 40 mV due to the lysine-lipid interactions and corresponded well to the amplitude of the slow phase in the boundary potential changes induced by polylysine adsorption on planar BLM. This phase can be attributed to polypeptide conformational changes and/or lipid bilayer restructuring phenomena.

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Section: Resultsmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Structural factors of lysine and polylysine interaction with lipid membranes

Marukovich

Nesterenko

Ermakov

2015

Biochem. Moscow Suppl. Ser. A

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“…The results obtained in [ 131 ] also indicate a decrease in membrane capacity C p with an increase in ionic strength I for medium and large polymers. A similar effect of ionic strength on the adsorption efficiency follows from experimental data [ 83 ] and numerical simulation results [ 87 ].…”

Section: Lateral Heterogeneity Of Polymer Layer As It Follows From the Analysis Of Electrokinetic Datamentioning

confidence: 62%

“…Since adsorption of large polycations and inorganic multivalent cations is largely determined by electrostatic interactions with the charged membrane surface, they demonstrate a similar shape of ζ-potential dependence on their contents measured in liposome suspension of a negatively charged lipid. The electrokinetic data in Figure 1 demonstrate this fact in the example of adsorption of trivalent cations of Gd 3+ [ 82 ] and relatively short polylysine molecules (12–20 subunits) [ 83 ] on the surface of liposomes composed of phosphatidylserine (PS). In both cases, there is a sharp rise of the curve near the point of zero charge where the surface is neutral, and the electrostatic interactions are minimized.…”

Section: Inorganic Cations and Polycations In The Electric Fields At The Membrane Boundarymentioning

confidence: 99%

Heterogeneity in Lateral Distribution of Polycations at the Surface of Lipid Membrane: From the Experimental Data to the Theoretical Model

2021

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Natural and synthetic polycations of different kinds attract substantial attention due to an increasing number of their applications in the biomedical industry and in pharmacology. The key characteristic determining the effectiveness of the majority of these applications is the number of macromolecules adsorbed on the surface of biological cells or their lipid models. Their study is complicated by a possible heterogeneity of polymer layer adsorbed on the membrane. Experimental methods reflecting the structure of the layer include the electrokinetic measurements in liposome suspension and the boundary potential of planar bilayer lipid membranes (BLM) and lipid monolayers with a mixed composition of lipids and the ionic media. In the review, we systematically analyze the methods of experimental registration and theoretical description of the laterally heterogeneous structures in the polymer layer published in the literature and in our previous studies. In particular, we consider a model based on classical theory of the electrical double layer, used to analyze the available data of the electrokinetic measurements in liposome suspension with polylysines of varying molecular mass. This model suggests a few parameters related to the heterogeneity of the polymer layer and allows determining the conditions for its appearance at the membrane surface. A further development of this theoretical approach is discussed.

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“…The zeta-potential values showed that the liposomal surface changed from electronegative to electropositive, strongly suggesting that, from a certain concentration, an effective adsorption of LSH by ε-polylysine molecules took place (Volodkin et al, 2007a,b;Sasaki et al, 2013). A plateau was reached from 0.5% upwards, indicating that liposomes were totally coated with PL at this concentration, preventing further adsorption of excess PL by saturation of the bilayer membrane binding sites (Volodkin et al, 2007a,b;Finogenova et al, 2008). Residual PL amino groups, which do not interact with the liposomal surface, would presumably be available to exert a direct antimicrobial effect.…”

Section: Liposome Complexation With ε-Polylysinementioning

confidence: 99%

A Novel Functional Wrapping Design by Complexation of ε-Polylysine with Liposomes Entrapping Bioactive Peptides

Alemán

Mastrogiacomo

López‐Caballero

et al. 2016

Food Bioprocess Technol

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An innovative edible wrapping with potential use for designing functional foods with antimicrobial capacity was developed by complexation of ε-polylysine with peptideloaded liposomes. Unmarketable long-term frozen cooked shrimp (L. vannamei) muscle was used as a source of both bioactive peptides and complex liposomal suspension carrier, producing a sustainable value-added protein wrapping material with desirable sensory properties. A <10 kDa peptide fraction (SH) with antioxidant and angiotensin-converting enzyme inhibitory capacity was encapsulated in partially purified phosphatidylcholine (PC) liposomes (LSH) with an entrapment efficiency of 85%. The average size and zeta-potential of LSH were 164 ± 2 nm and-37.0 ± 1.7 mV, respectively. The LSH surface changed from electronegative to electropositive upon adsorption of ε-polylysine (PL) with an optimal concentration of 0.5%. The average diameter and zeta-potential of the resulting complex ε-polylysine-adsorbed liposomes containing the peptide hydrolysate (PL-LSH) were 216 ± 5 nm and +51.1 ± 1.1 mV, respectively. The ε-PL proved to be effective as liposome stabilizing and antimicrobial agent. The PL-LSH suspension was incorporated in the formulation of the protein wrapping to provide it with both bioactive and antimicrobial properties. The wrapping showed low water solubility (≈ 30%) and low mechanical resistance (tensile strength = 0.23 ± 0.06 MPa; elongation at break = 0.91 ± 0.19 %) properties that allowed it to be very versatile for varied food design, and was effective against Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Yersinia enterocolitica.

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Electrostatic effects upon adsorption and desorption of polylysines on the surface of lipid membranes of different composition

Cited by 11 publications

References 17 publications

Structural factors of lysine and polylysine interaction with lipid membranes

Structural factors of lysine and polylysine interaction with lipid membranes

Heterogeneity in Lateral Distribution of Polycations at the Surface of Lipid Membrane: From the Experimental Data to the Theoretical Model

A Novel Functional Wrapping Design by Complexation of ε-Polylysine with Liposomes Entrapping Bioactive Peptides

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