Electrostatic self-assembled graphene oxide-collagen scaffolds towards a three-dimensional microenvironment for biomimetic applications

Girão, André F.; Gonçalves, Gil; Bhangra, Kulraj Singh; Phillips, James B.; Knowles, Jonathan C.; Irurueta, Gonzalo; Singh, Manoj K.; Bdkin, Igor; Completo, António; Marques, Paula A.A.P.

doi:10.1039/c6ra10213a

Cited by 40 publications

(36 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The non-reduced composites present an initially high swelling ratio (Q), followed by a gradual decrease in absorption with time indicating water loss, reaching the equilibrium stage after approximately 3 h. For the reduced composites, the opposite profile was observed, i.e., the swelling gradually increases until the equilibrium after 4 h, reaching lower Q values than the previous series. The same tendency was verified by Girão et al (Girão et al, 2016) for collagen/graphene oxide scaffolds. For the non-reduced foams, the swelling behaviour is higher for the BC/GO50 than for the BC/GO25 series, most probably due to a structure collapse that closes the pores.…”

Section: Dimensional Stability Of the Bc/go Foamssupporting

confidence: 76%

“…The final blends were placed in a cylindrical mould, frozen at -20 ºC and freeze-dried in a LyoQuest-Telstar freeze dryer. The samples prepared with 1.0 µL of DMSO were reduced using two different approaches, which involve liquid and gas phase reduction of the material in presence of (N2H4) (Girão et al, 2016) or NH3 (Sandoval et al, 2016). In the first case, the aerogels were immersed into a (N2H4) solution (1µL/mL) at RT for 24h.…”

Section: Synthesis Of Bc/go Aerogelsmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial cellulose/graphene oxide aerogels with enhanced dimensional and thermal stability

Pinto

Gonçalves

Sandoval

et al. 2020

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Dimensional Stability Of the Bc/go Foamssupporting

confidence: 76%

Section: Synthesis Of Bc/go Aerogelsmentioning

confidence: 99%

Bacterial cellulose/graphene oxide aerogels with enhanced dimensional and thermal stability

Pinto

Gonçalves

Sandoval

et al. 2020

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a study by Shin et al, it was demonstrated that a GO–PLGA–Col scaffold showed no toxicity and the composite matrix with concentrations of 10,000 μg/mL also exhibited cell attachment and proliferation better than that of PLGA‐Col composites . Two recent studies showed no toxicity for Col–GO scaffolds with GO concentrations of 1000 and 2000 μg/mL . More research is necessary to overcome the contradiction of results on the biocompatibility of different concentrations of graphene‐based nanomaterials, such as GO.…”

Section: Discussionmentioning

confidence: 99%

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Norahan

Amroon

Ghahremanzadeh

et al. 2018

J Biomedical Materials Res

105

View full text Add to dashboard Cite

The application of a cardiac patch over the epicardial surface has shown positive effects in protecting cardiac function postinfarction. Electroactive patches could enhance electrical signal propagation among cardiac cells. In the present study, an electrically active composite of collagen and graphene oxide (Col-GO) was fabricated as a cardiac patch. Col scaffolds were fabricated using a freeze-drying method and coated covalently with GO. Some scaffolds were also reduced by a reduction agent to restore the high conductivity of GO. GO was shown to be a single layer with suitable lateral size for biological application. The Col-GO scaffolds contained randomly oriented interconnected pores with appropriate pore sizes of 120-138 AE 8 μm. GO flakes were also well distributed in the pore walls. By increasing the GO concentration, the tensile strength of the scaffolds was enhanced from 75 kPa for Col-GO-5 to 162 kPa for Col-GO-90. Young modulus also followed the same trend. Electrical conductivity of the scaffolds was in the range of semi-conductive materials (~10 −4 S/m), which is suitable for cardiac tissue engineering applications. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated no toxic effects on human umbilical vein endothelial cells (HUVECs) after 96 h. Also, a 10-days degradation product of the samples was compatible with HUVECs. Reduced scaffolds supported neonatal cardiomyocyte adhesion and upregulated the expression of the cardiac genes, including Cx43, Actin4, and Trpt-2 than their nonconductive counterparts. The obtained results confirmed the angiogenic properties of reduced Go-containing materials for cardiovascular applications where angiogenesis plays an important role, especially postinfarction.

show abstract

“…The final thickness of the fibrous mesh was approximately 100 μm. To guarantee the vertical alignment of the deep layer, small rectangles (length = 4 cm; width = 2 mm) were cut from the electrospun mesh and then rolled into spiralled cylinders within a GO-collagen hydrogel, which was fabricated accordingly to our previous work 41 . Briefly, the GO aqueous dispersion was directly mixed with the collagen solution in acidic medium (pH =2) and then rapidly shaken for 10 s in order to form a hydrogel.…”

Section: Pcl-go-collagen Scaffolds Fabricationmentioning

confidence: 99%

Mimicking nature: Fabrication of 3D anisotropic electrospun polycaprolactone scaffolds for cartilage tissue engineering applications

Girão

Semitela

Ramalho

et al. 2018

Composites Part B: Engineering

Self Cite

View full text Add to dashboard Cite

There is a growing need to develop strategies capable of engineering the anisotropic cartilagino us fibrous network in vitro and consequently overcome the anatomical and functional restrictions of the standard medical procedures used for cartilage regeneration. In this work, we suggest a fabrication procedure that uses two complementary electrospinning set ups to build 3D multilayered fibrous scaffolds with adjustable fibre orientation features, matching the topographic cues of the three cartilaginous zones. Polycaprolactone (PCL) was used as bulk material for the differe nt electrospun layers (horizontally, randomly and vertically aligned) that were assembled and then structurally maintained by a biocompatible graphene-oxide-collagen (GO-collagen) microporous network. To validate the resourcefulness of the technique, four PCL-GO-collagen scaffolds with different anisotropic properties were produced and characterized by analysing their depth dependent morphological and mechanical anisotropy. The results confirmed that each electrospun layer presents a similar topography relatively to its natural counterpart and that changing the fibre orientation modifies the compressive behaviour of the scaffold, which is specially influenced by the presence of the vertically aligned fibres. Overall, the presented fabricating technique offers the opportunity to easily tune the anisotropy of 3D electrospun scaffolds towards the enhancement of both static and dynamic cell culture protocols concerning cartilage tissue engineering (TE).

show abstract

Electrostatic self-assembled graphene oxide-collagen scaffolds towards a three-dimensional microenvironment for biomimetic applications

Abstract: The manipulation of the interactions between the cationic amine groups from collagen and the anionic carboxylic groups from graphene oxide mediate the synthesis of a self-assembled hydrogel capable of generate suitable 3D cellular microenvironments.

Cited by 40 publications

References 67 publications

Bacterial cellulose/graphene oxide aerogels with enhanced dimensional and thermal stability

Bacterial cellulose/graphene oxide aerogels with enhanced dimensional and thermal stability

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Mimicking nature: Fabrication of 3D anisotropic electrospun polycaprolactone scaffolds for cartilage tissue engineering applications

Contact Info

Product

Resources

About