Electrosynthesized Surface‐Imprinted Conducting Polymer Microrods for Selective Protein Recognition

Menaker, Anna; Syritski, Vitali; Reut, Jekaterina; Öpik, Andres; Horváth, Viola; Gyurcsányi, Róbert E.

doi:10.1002/adma.200803597

Cited by 136 publications

(89 citation statements)

References 38 publications

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“…Subsequent removal of the template leads to recognition sites in the molecularly imprinted polymer (MIP) that can selectively rebind the target. Extending this principle to macromolecules such as proteins necessitated the implementation of a variety of enabling technologies (e.g., epitope imprinting, [4,5] surface imprinting [6][7][8][9][10]) to cope both with the fragility of the target [11] as well as with its limited diffusivity in the cross-linked polymeric matrices. The electrochemical polymerization in this respect offers clear advantages such as performing the polymerization in aqueous conditions that is compatible with the proteinaceous target.…”

Section: Introductionmentioning

confidence: 99%

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Stojanović

Erdőssy

Keltai

et al. 2017

Analytica Chimica Acta

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Molecularly imprinted polymers (MIPs) rendered selective solely by the imprinting with protein templates lacking of distinctive properties to facilitate strong target-MIP interaction are likely to exhibit medium to low template binding affinities. While this prohibits the use of such MIPs for applications requiring the assessment of very low template concentrations, their implementation for the quantification of high-abundance proteins seems to have a clear niche in the analytical practice. We investigated this opportunity by developing a polyscopoletin-based MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. As reference for low abundance protein ferritin-MIPs were 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 also prepared by the same procedure. Under optimal conditions, the imprinted sensors gave a linear response to HSA in the concentration range of 20-100 mg/dm 3 , and to ferritin in the range of 120-360 mg/dm 3 . While as expected the obtained limit of detection was not sufficient to determine endogenous ferritin in plasma, the HSA-sensor was successfully employed to analyse urine samples of patients with albuminuria. The results suggest that MIP-based sensors may be applicable for quantifying high abundance proteins in a clinical setting.

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Section: Introductionmentioning

confidence: 99%

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Stojanović

Erdőssy

Keltai

et al. 2017

Analytica Chimica Acta

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“…The initial increase in the current density indicates the instantaneous formation of a thin layer of conducting polymer underneath the membrane on the electrode from the penetrated monomer solution [39]. When the monomers are depleted from this thin layer the growth of the polymer is confined exclusively to the pores.…”

Section: Magnetization Measurement Of the Polymer Microrods And The Fmentioning

confidence: 99%

“…While all these techniques are based on chemical polymerization we have recently introduced new strategies to surface imprint electrosynthesized electrically conducting polymers with biomacromolecules [38,39]. These are based on sacrificial template synthesis using various geometry microreactors with their inner wall modified with the target protein to generate surface imprinted polymers.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical template synthesis of protein-imprinted magnetic polymer microrods

et al. 2013

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A novel method for the electrochemical template synthesis of surface-imprinted magnetic polymer microrods for protein recognition is proposed. The polymer was electrodeposited into sacrificial cylindrical microreactors, the internal walls of which were previously modified with a target model protein, avidin, by simple physisorption.The electropolymerization was performed from a mixture of 3,4-ethylenedioxythiophene, poly(styrenesulfonate) (PSS) and PSS-coated superparamagnetic nanoparticles resulting in the formation of inherently electroconductive polymers confined to the volume of the microreactor. Here we show that: (i) the template synthesis within cylindrical microreactors results in polymer rods with dimensions matching that of the reactor, (ii) the incorporation of superparamagnetic particles induces magnetic properties that allow for efficient collection and manipulation of the microrods released from the microreactors in magnetic field even from dilute solution, (iii) the protein coating on the internal walls of the microreactors is shown to generate molecular imprints on the surface of the polymeric rods. This latter property was demonstrated by comparative binding experiments of a fluorescent avidin derivative to the surface-imprinted and non-imprinted magnetic polymer microrods.

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“…To simplify this process, Menaker et al 166 presented a new approach; instead of nanoporous alumina membranes, polycarbonate membrane filter with cylindrical pores (F = 8 mm) was used as a sacrificial microreactor since polycarbonate membrane is hydrophobic in nature and can adsorb protein molecules, which offers a straightforward method for fixing the target protein on the pore walls by simple physical adsorption.…”

Section: Protein Imprintingmentioning

confidence: 99%

Recent advances in molecular imprinting technology: current status, challenges and highlighted applications

2011

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Molecular imprinting technology (MIT) concerns formation of selective sites in a polymer matrix with the memory of a template. Recently, molecularly imprinted polymers (MIPs) have aroused extensive attention and been widely applied in many fields, such as solid-phase extraction, chemical sensors and artificial antibodies owing to their desired selectivity, physical robustness, thermal stability, as well as low cost and easy preparation. With the rapid development of MIT as a research hotspot, it faces a number of challenges, involving biological macromolecule imprinting, heterogeneous binding sites, template leakage, incompatibility with aqueous media, low binding capacity and slow mass transfer, which restricts its applications in various aspects. This critical review briefly reviews the current status of MIT, particular emphasis on significant progresses of novel imprinting methods, some challenges and effective strategies for MIT, and highlighted applications of MIPs. Finally, some significant attempts in further developing MIT are also proposed (236 references).

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Electrosynthesized Surface‐Imprinted Conducting Polymer Microrods for Selective Protein Recognition

Cited by 136 publications

References 38 publications

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Electrochemical template synthesis of protein-imprinted magnetic polymer microrods

Recent advances in molecular imprinting technology: current status, challenges and highlighted applications

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