Elektronenmikroskopie des menschlichen Nebenhodenepithels

Horstmann, Ernst

doi:10.1007/bf00410231

Cited by 74 publications

(17 citation statements)

References 13 publications

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“…The structure of the principal cell of the normal rat cauda epididymis is similar to that described previously (14), for the cauda epididymis in other species (15,16), and for the rat vas deferens (2,17) . A section from the normal rat cauda epididymis is shown in Fig.…”

Section: Morphologysupporting

confidence: 82%

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Cytochemical Staining of Multivesicular Body and Golgi Vesicles

Friend

1969

The Journal of Cell Biology

264

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To investigate the origin and nature of vesicles found within multivesicular bodies (mvb), the cytochemical staining properties of mvb vesicles were compared with those of other cytoplasmic vesicles, i.e. those associated with the Golgi complex and endocytic vesicles found near the apical cell surface. Rat epididymal tissue was stained in unbuffered OsO4 for 40–48 hr, and the distribution of stain was compared to that of reaction products for acid phosphatase (AcPase) to mark lysosomal vesicles, or thiamine pyrophosphatase (TPPase) to mark certain Golgi vesicles, or infused with peroxidase (HRPase) to demonstrate endocytic vesicles. Mvb vesicles were stained only by OsO4; AcPase, TPPase, and HRPase reaction products stained the mvb matrix. OsO4 also stained certain vesicles along the convex surface of the Golgi complex. The findings suggest that mvb vesicles in epididymal epithelium are not lysosomes and are not involved in protein uptake. The majority of these vesicles have cytochemical reactions in common with vesicles located along the convex surface of the Golgi complex and may be derived therefrom. A minority are derived from the mvb-limiting membrane.

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Section: Morphologysupporting

confidence: 82%

“…"Compaction" of the membranes with the loss of 0s04-staining content of this cisterna and the budding of vesicles from the mvb membrane invaginations (arrows) (Fig . 15) would result in the formation of an mvb like that depicted in Fig . 16 . In this mvb, most of the vesicles are stained with 0804, but two are not .…”

Section: Nature and Origin Of Mvb Vesiclesmentioning

confidence: 99%

Cytochemical Staining of Multivesicular Body and Golgi Vesicles

Friend

1969

The Journal of Cell Biology

264

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“…There was no indication of a secretion from the apical cell surface into the lumen. The buds of the apical cytoplasm extending to the lumen were described as an indication of apocrine secre tion [Horstmann, 1962]. But those apical buds which may contain pinocytotic vesicles, multivesicular bodies and other lysosomal dense bodies appear to be related to absorption.…”

Section: Discussionmentioning

confidence: 99%

“…the epithelial cells of the epididymis have an absorbing capacity [Shaver, 1954: Burgos. 1964: Nicander, 1965], a secretory function of the apocrine type [Horstmann, 1962] or of the holocrine type [Marian and Risley, 1963;Marian and Allen, 1964], they produce and secrete glycerylphosphorylcholine [Scon el a!., 1963], mucus [Kreth. 1965], carnitine [Marquis and Fritz, 1965], sialic acid [Rajalakshmi and Prasad, 1969] or in tissue culture cholesterol and testosterone [Hamilton and Fawcett, 1970], take up sperm and parts of sperm in vivo after vasectomy [Flickinger, 1972;Alexander, 1973], in (issue culture [Hoffman el at., 1976] and affect sperm maturation [Marian and Allen.…”

Section: Introductionmentioning

confidence: 99%

An electron microscopical study of epididymal epithelium of rats treated with gonadotropic hormone

Erkoçak¹

1978

Cells Tissues Organs

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The ultrastructural modifications of the epithelial cells of rat corpus epididymis stimulated with gonadotropic hormone were studied. The structural variety of the cells depending on functional conditions becomes more prominent 6 h after the injection of gonadotropic hormone. Light large cells have one or often two nucleus-containing big nucleoli, in their cytoplasm there are numerous vesicles, a well-developed Golgi apparatus, other organelles and lysosomal bodies. Some other cells are filled with many large vacuoles of different density, dense bodies and vesicles. Cells of another type which are in the majority show an unusually active structure reflecting the function of synthesis. The more prominent nucleolus is associated to clumps of chromatin. Their apical cytoplasm is filled by a structure related to absorption. The whole remaining part of their cytoplasm is covered with a very extensive Golgi apparatus and a very well developed granular endoplasmic reticulum. The extremely enlarged cisternae of this reticulum were found to be very closely applied to the basal cell membrane. There is a flocculent material inside the cisternae. Similar material is observed in the extracellular medium under the basal membrane. The epithelium seems normal 10 h after the injection of hormone, but large light cells make up the majority of them.

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“…

Electron microscope studies of the vasa deferentia and epididymides of various animals have emphasized the endoplasmic reticulum of the epithelial cell to a greater or lesser degree (Nicander & Schantz, 1961;Horstmann, 1962;Leeson & Leeson, 1964;Schmidt, 1964;Holstein, 1964). Recent studies (Hamilton, Jones & Fawcett, 1969; Hamilton, 1971a, b) (Finck, 1960) or Spurr low viscosity embedding media (Spurr, 1969

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mentioning

confidence: 99%