Elektronenmikroskopische Untersuchungen am Virus der Stomatitis vesicularis

Reczko, E

doi:10.1007/bf01241638

Cited by 33 publications

(9 citation statements)

References 8 publications

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“…(Table 1 ; 2 and 10). At the morphological level the data of several groups agree closely and support the general structure (Reczko, 1960: Stone, Sellers & Hiramoto 1961: Howatson & Whitmore, 1962) shown schematically in Fig. 2.…”

Section: Discussionsupporting

confidence: 62%

The Morphology of Vesicular Stomatitis Virus (Indiana C) Derived from Chick Embryos or Cultures of BHK21/13 Cells

Bradish

Kirkham

1966

Journal of General Microbiology

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SUMMARYThe virus of vesicular stomatitis is shown to exist as a system of several particle forms. Two major particles, the 'bullet' and 'cap', appear in electron micrographs in various states as outer structural layers are absent or lost. High infectivity is not associated with particles of one form. The distribution of particle forms is apparently determined by cultural conditions and is not significantly modified by the preparative procedures employed.The morphology of the several components of the virus system is described.

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Section: Discussionsupporting

confidence: 62%

The Morphology of Vesicular Stomatitis Virus (Indiana C) Derived from Chick Embryos or Cultures of BHK21/13 Cells

Bradish

Kirkham

1966

Journal of General Microbiology

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“…Although ultrastructural changes of cells in culture infected with wt VSV have been reported, most studies have been associated with the intracellular site of virus maturation (Reczko, 1961;Howatson & Whitmore, 1962;Coward et aL, 1971;Faulkner et aL, 1979). Several investigators have observed intracellular vacuoles that appeared during virus assembly (Mussgay & Weibel, 1963;David-West & Labzoffsky, 1968;Zajac & Hummeler, 1970;Zee et al, 1970).…”

Section: Introductionmentioning

confidence: 99%

Cytopathic Effects in Mouse Neuroblastoma Cells during a Non-permissive Infection with a Mutant of Vesicular Stomatitis Virus

Dille

Hughes

Johnson

et al. 1981

Journal of General Virology

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SUMMARYMorphological changes were extensive following infection of murine neuroblastoma N-18 cells with a temperature-sensitive (ts) mutant of vesicular stomatitis virus (VSV), G31 (complementation group III), and incubation at 39 °C, a non-permissive condition for virion maturation. Incubation for 24 h after infection resulted in extensive morphological degeneration of mitochondria with over 80 % of the mitochondria having degenerated. Mitochondrial function, as determined by Janus green B supravital staining, was reduced by 81% from that in uninfected cells. Cellular ATP levels were reduced by 50% 12 h after infection. Mitochondrial degeneration still occurred in infected cells after the inactivation of lysosomes with chloroquine. Extensive cell fusion and cytoplasmic vacuole formation also occurred during the non-permissive infection with ts G31. Loss of plasma membrane integrity was not the cause of vacuole formation since 90 % of the cells were able to exclude trypan blue 24 h after infection, nor were the vacuoles the result of inactivation of the mitochondria since cyanide-poisoned cells did not form vacuoles. The cytopathic alterations observed in N-18 cells during the non-permissive infection of N-18 cells with ts G31 did not occur during the non-permissive infection of N-18 cells with ts Gll (I), ts G41 (IV), or u.v.-inactivated ts G31. However, the non-permissive infection with ts O45(V) led to mitochondrial degeneration and cytoplasmic vacuole formation, but no cell fusion occurred. These results are discussed in light of the ultrastructural features previously observed in the central nervous system of mice infected with ts G31 and cells in culture infected with wild-type VSV.

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“…Furthermore, Bradish et al (2) found non-infectious spherical particles, 65 mg in diameter, possessing a virusspecific complement-fixing activity. By negative staining with phosphotungstic acid, it was shown (3,4) that each rod had a round and a square end, and there was evidence of an internal component, probably helical (4). In many virus particles, a central channel of variable depth was seen (4), and in some rods an "inclusion" was visible (3).…”

mentioning

confidence: 99%

“…In chorioallantoic membranes of eggs infected with VS virus, cells of the chorionic and allantoic layers bordering the mesoderm revealed granular areas on their surface. Only in these areas, which were separated from the cytoplasm by a membrane, were virus rods and 52-m~t particles visible (3). From studies of VS virus-infected L cells (4), it was concluded that the virus particles were assembled at the cell membrane.…”

mentioning

confidence: 99%

“…By negative staining with phosphotungstic acid, it was shown (3,4) that each rod had a round and a square end, and there was evidence of an internal component, probably helical (4). In many virus particles, a central channel of variable depth was seen (4), and in some rods an "inclusion" was visible (3). On the basis of x-ray inactivation studies of extracellular virus particles, a radiation-sensitive sphere with a diameter between 36 and 47 m# was assumed (5).…”

mentioning

confidence: 99%

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Electron Microscopic Studies on the Development of Vesicular Stomatitis Virus in Kb Cells

Mussgay

Weibel

1963

The Journal of Cell Biology

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The development of vcsicular stomatitis virus in KB cells was studied by electron microscopy. Sections of infected cells were made l, 4, 7, 10, and 20 hours after inoculation of the cell cultures, and at the same intervals the supcrnatant fluid was assayed for virus titcr by the plaque test in chick embryo cells. At 10, 14, and 20 hours after inoculation, virus rods were observed attached to cytoplasmic membranes, inside cytoplasmic vacuoles, and attached to the membranes delimiting these vacuoles; they were also found on the surface membrane of the cclls. Besides the rods, spherical particles of different sizes and shapes were seen. The possibility that these structures arc related to the development of virus rods is discussed. A similarity was noted between the site of maturation of vesicular stomatitis virus rods and that of some other arbor viruses.Vesicular stomatitis (VS) virus particles are rodshaped. Their dimensions have been reported to be 210 )< 60 (1) or 175 )< 69 mg (2). Furthermore, Bradish et al. (2) found non-infectious spherical particles, 65 mg in diameter, possessing a virusspecific complement-fixing activity. By negative staining with phosphotungstic acid, it was shown (3, 4) that each rod had a round and a square end, and there was evidence of an internal component, probably helical (4). In many virus particles, a central channel of variable depth was seen (4), and in some rods an "inclusion" was visible (3). On the basis of x-ray inactivation studies of extracellular virus particles, a radiation-sensitive sphere with a diameter between 36 and 47 m# was assumed (5).Rods and 52-m# spherical particles were observed in ultrathin sections of the mouth mucosa taken from calves infected with VS virus (6). Both components were seen only in the intercellular space. In chorioallantoic membranes of eggs infected with VS virus, cells of the chorionic and allantoic layers bordering the mesoderm revealed granular areas on their surface. Only in these areas, which were separated from the cytoplasm by a membrane, were virus rods and 52-m~t particles visible (3). From studies of VS virus-infected L cells (4), it was concluded that the virus particles were assembled at the cell membrane.In studies on the growth of VS virus in chick embryo cells and monkey kidney cells, Franklin (5) demonstrated that virus was released by a continuous process; the eclipse phase was between 1 and 2 hours and the release time approximately 2 to 3 minutes.Our main interest was to study the development of VS virus in KB cells and to check whether at an early stage mature rods can be found inside the cells or only at or near the surface membrane.

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Elektronenmikroskopische Untersuchungen am Virus der Stomatitis vesicularis

Cited by 33 publications

References 8 publications

The Morphology of Vesicular Stomatitis Virus (Indiana C) Derived from Chick Embryos or Cultures of BHK21/13 Cells

The Morphology of Vesicular Stomatitis Virus (Indiana C) Derived from Chick Embryos or Cultures of BHK21/13 Cells

Cytopathic Effects in Mouse Neuroblastoma Cells during a Non-permissive Infection with a Mutant of Vesicular Stomatitis Virus

Electron Microscopic Studies on the Development of Vesicular Stomatitis Virus in Kb Cells

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