continuous-flow isoelectric focusing apparatus to give smooth laminar flowDetails are given of a method of casting multichannel tubing in silicone rubber in the form of a slab 2 mm thick and up to 380 mm long. Up to 264 parallel channels were formed at 0.8 mm centers along the length of the material, each channel being 0.4 mm in diameter. The material was used both as the outlet manifold in an apparatus for continuous-flow isoelectric focusing (IEF), the construction of which is described, and also in conjunction with a slow-running peristaltic pump as multichannel pump-tubing when it was found that at a flow-rate of about 0.3 ml h-' per channel, the variation between channels was less than 0.6 %. The coupling of the pump-tubing to the outlet manifold of the separation cell of the IEF apparatus resulted in a very smooth laminar flow of liquid in the separation cell itself.