Element‐specific detectors for highpressure liquid chromatography (HPLC): acomputerized, automated HPLC‐graphitefurnace atomic absorption system

Haswell, Stephen J.; Stockton, R. A.; Bancroft, K. C. C.; O’Neill, Peter; Rahman, Anisur; Irgolic, Kurt J.

doi:10.1155/s1463924687000026

Cited by 15 publications

(3 citation statements)

References 4 publications

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“…In this paper, we obtained the main active components of YZP by HPLC combined with public databases. HPLC has been widely used in various fields of qualitative and quantitative analysis because of its advantages such as high analysis speed, simple operation, high sensitivity, good reproducibility, and wide application range [48,49]. However, HPLC has its limitations, for example, it cannot detect volatiles and provide accurate structures of compounds, and it is difficult to detect low levels of active components.…”

Section: Discussionmentioning

confidence: 99%

Active Compounds and Targets of Yuanzhi Powder in Treating Alzheimer’s Disease and Its Relationship with Immune Infiltration Based on HPLC Fingerprint and Network Pharmacology

Liu

Wang

Hao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Yuanzhi powder (YZP) has been extensively investigated as a natural prescription with therapeutic benefits for Alzheimer’s disease (AD). However, its active compounds and underlying immune mechanism for treating AD are still unclear. This study aimed to investigate the immune mechanism of YZP against AD through high-performance liquid chromatography (HPLC)-based network pharmacology and gene chip technology. Methods. Active components of YZP were obtained from HPLC and public databases. Subsequently, GSE5281, GSE28146, GSE29378, and GSE97760 from the Gene Expression Omnibus (GEO) database were downloaded to extract AD difference genes (DEGs). The active components-targets network and protein interaction network were then constructed by Cytoscape. The biological processes and signaling pathways, which implicate the targets of YZP for AD, were analyzed using the ClueGo Cytoscape plug-in. Molecular docking experiments were performed to verify the affinity of targets and ligands. Ultimately, the link between the hub genes and immune cell infiltration was assessed via CIBERSORT. Results. 83 YZP active compounds and 641 DEGs associated with AD, including quercetin, berberine, 3,6′-disinapoylsucrose, coptisine, and palmatine, were evaluated. We showed that FOS, CCL2, and GJA1 were the core targets and that the gap junction is an essential signaling pathway in YZP for AD. Furthermore, the AD group had a higher infiltration level of naïve B cells and resting CD4 memory T cells, as determined by the CIBERSORT. Notably, the immune cells-targets network demonstrates that GJA1 and GRM1 are intimately related to naïve B cells and plasma cells. Conclusions. YZP may help treat AD by targeting proteins with key active compounds to regulate naïve B cells and plasma cells. Our results demonstrate a new immune mechanism for treating AD with YZP.

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Section: Discussionmentioning

confidence: 99%

Active Compounds and Targets of Yuanzhi Powder in Treating Alzheimer’s Disease and Its Relationship with Immune Infiltration Based on HPLC Fingerprint and Network Pharmacology

Liu

Wang

Hao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Thus, the developed integrated SEC-GFAAS system can now be used to quantify the Hp-Hb complex in plasma, which has important diagnostic ramifications since a variety of human diseases can result in the lysis of red blood cells [11,13]. The developed methodology is of potential interest to clinical laboratories since it has been previously demonstrated that HPLC-GFAAS systems can be computer controlled to obtain fully automated analysis systems [24,32]. Considering that the exposure of red blood cells to Hg 2+ and Cd 2+ has been demonstrated to contribute to their lysis [33,34], the analysis of human plasma for the Hp-Hb complex may provide insight into the exposure of human populations to toxic metals [35], which is projected to affect more people in the future [36,37].…”

Section: Discussionmentioning

confidence: 99%

Sample preparation of blood plasma enables baseline separation of iron metalloproteins by SEC-GFAAS

Miller

Sarpong‐Kumankomah

Egorov³

et al. 2020

Journal of Chromatography B

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The analysis of human plasma for biomarkers holds promise to revolutionize disease diagnosis, but is hampered by the inherent complexity of the plasma proteome. One way to overcome this problem is to analyze plasma for a sub-proteome, such as the metalloproteome. Previous studies employing size-exclusion chromatography (SEC) coupled on-line to an inductively coupled plasma-atomic emission spectrometer (ICP-AES) have revealed that plasma contains ~12 copper, iron and zinc metalloproteins. This included the iron metalloproteins transferrin (Tf) and a recently identified haptoglobin-hemoglobin (Hp-Hb) complex, which is formed in plasma when red blood cells rupture. Since this SEC-ICP-AES method required a sample volume of 500 µL to generate diagnostically useful results, we sought to develop an alternative SEC-based hyphenated approach using a smaller SEC column (150 x 5 mm I.D.) and a graphite furnace atomic absorption spectrometer (GFAAS) as the iron-specific detector. A designed interface enabled the integration of the SEC system with the GFAAS. Baseline separation between the Hp-Hb complex and Tf was achieved by developing a sample preparation procedure which involved the chelating agent-based mobilization of Fe from Tf to a small molecular weight Fe complex. Spiking of human plasma (1.0 mL) with red blood cell lysate (1-2 µL) increased only the intensity of the Fe peak corresponding to the Hp-Hb complex, but not that of Tf. Since the developed SEC-GFAAS method requires only 50 µL for analysis, it can now be employed for the cost-effective quantification of the clinically relevant Hb-Hp complex in human plasma in <50 min.

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“…These compounds differ in their physical and chemical properties: some are volatile, neutral molecules (Me4Pb, Me2Se, Me:As), some are involatile and carry a charge However, the sample (generally a solution) must be dried and ashed prior to atomization. These sequential steps prevent the direct coupling of a continuously working chromatograph with the graphite furnace atomic absorption spectrometer: several systems have been developed to overcome this difficulty [2][3][4][5][6][7][8][9] and these methods were recently summarized [10 and 11]. Brinckman and coworkers [3] developed a system based on autosamplers: the effluent is routed through a flow-through cup from which the autosampler periodically transfers an aliquot into the furnace.…”

Section: Introductionmentioning

confidence: 99%

Computerized data treatment for an HPLC‐GFAAS system for the identification and quantification of trace element compounds

Kölbl

Kalcher

Irgolic

1993

Journal of Analytical Methods in Chemistry

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Liquid chromatographs, coupled with graphite furnace atomic absorption spectrometers, have been widely used for the identification and quantification of trace element compounds. The quantification of the discontinuous signals from the spectrometer defining a chromatographic band is very much a matter of judgement and therefore prone to error. This paper describes a system which links a high-performance liquid chromatograph via a ‘Brinckman’ flowthrough cup to a Hitachi Zeeman graphite furnace atomic absorption spectrometer equipped with an autosampler. The introduction of aliquots from the column effluent and the analysis sequence is computer-controlled through a home-built interface. The signals from the spectrometer are passed through an analoguedigital converter and processed by selectable algorithms. The software offers a variety of options for processing the chromatographic data, such as data smoothing, Gaussian or spline interpolation, and trapezium or Simpson integration. This system was used to separate and determine selenite and selenate in aqueous solution with absolute detection limits (3 σ) of 23 ng Se for selenite and 16 ng Se for selenate. This system can be adapted to other spectrometers, provided that the required connections to the electronics can be made.

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Element‐specific detectors for highpressure liquid chromatography (HPLC): acomputerized, automated HPLC‐graphitefurnace atomic absorption system

Cited by 15 publications

References 4 publications

Active Compounds and Targets of Yuanzhi Powder in Treating Alzheimer’s Disease and Its Relationship with Immune Infiltration Based on HPLC Fingerprint and Network Pharmacology

Active Compounds and Targets of Yuanzhi Powder in Treating Alzheimer’s Disease and Its Relationship with Immune Infiltration Based on HPLC Fingerprint and Network Pharmacology

Sample preparation of blood plasma enables baseline separation of iron metalloproteins by SEC-GFAAS

Computerized data treatment for an HPLC‐GFAAS system for the identification and quantification of trace element compounds

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