Elemental and immunohistochemical analysis of the lungs and hilar lymph node in a patient with asbestos exposure, a pilot study

Koga, Yasuhiko; Satoh, Takahiro; Kaira, Kyoichi; Koka, Masashi; Hisada, Takeshi; Hirato, Junko; Altan, Bolag; Yatomi, Masakiyo; Ono, Akiko; Kamide, Yosuke; Shimizu, Yasuo; Aoki‐Saito, Haruka; Tsurumaki, Hiroaki; Shimizu, Kimihiro; Mogi, Akira; Ishizuka, Tamotsu; Yamada, Masanobu; Dobashi, Kunio

doi:10.1007/s12199-016-0576-5

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…27 Furthermore, overexposure to silica/silicates is a significant risk factor for rapidly progressive pneumoconiosis. 29 Our PIXE analysis of lungs with asbestos demonstrated silica/silicates accumulation in the mediastinal lymph nodes, 15 and SEM with EDX analysis showed that inhaled silica accumulates in the mediastinal lymph nodes in patients with IPF. 19 A recent study showed that mediastinal lymphadenopathy (>10 mm) affects the prognosis in interstitial lung disease.…”

Section: ■ Materials and Methodsmentioning

confidence: 79%

“…In-air micro-PIXE was developed to obtain distribution maps of trace elements by the detection of characteristic X-rays emitted from trace elements. 15 An external proton beam is irradiated through thin polycarbonate film onto the tissue sample in an atmosphere in the in-air PIXE analysis. A sulfur (S) correction was made, as S can be a reliable indicator of tissue mass.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…2,10,14 In-air micro-particle-induced X-ray emission (PIXE) has recently been applied to measure the composition and concentrations of elements in biological samples in a noninvasive and nondestructive manner with higher elemental sensitivity and spatial resolution. 15,16 Using in-air micro-PIXE, we investigated accumulated silica and aluminum levels in the hilar lymph nodes of a patient with asbestos exposure 15 and detected magnesium, aluminum, and silica levels in the lungs of patients with idiopathic interstitial pneumonia (IIP) without dust exposure. 16 We hypothesized that an elemental analysis of the lungs of patients affected by IPF will show a significant association between inorganic compound presence and pulmonary function decline or IPF prognosis.…”

Section: ■ Introductionmentioning

confidence: 99%

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Progression of Idiopathic Pulmonary Fibrosis Is Associated with Silica/Silicate Inhalation

Koga

Satoh

Kaira

et al. 2021

Environ. Sci. Technol. Lett.

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Recently, environmental dust exposure has emerged as a possible risk factor for interstitial pneumonia. Inhaled silica/silicates and titanium are associated with the progression of rapidly progressive pneumoconiosis. However, the relationship between inhaled elements and idiopathic pulmonary fibrosis (IPF) remains unclear. In this study, we focused on IPF presenting with histological usual interstitial pneumonia and investigated the relationship between inhaled elements and IPF progression. We examined the elements in the lungs using in-air micro-particle-induced X-ray emission analysis (PIXE). Lung tissue samples with IPF were analyzed and compared with control lung samples. There was a significant difference between IPF and the control lungs in terms of silicon, assumed to form silica/silicates. Inhaled silicon in the lungs significantly correlated with the annual decline in forced vital capacity in patients with IPF. Furthermore, IPF patients with higher accumulation of silicon showed a significantly poorer prognosis than those with lower accumulation of silicon in the lungs. These significant differences were also seen in multivariate analyses, including smoking history, IPF severity, and/or antifibrotic treatment. In-air micro-PIXE can be beneficial for detecting inhaled elements in the lung. These results indicate that inhaled silica/silicates in the lung may be partly involved in the progression of IPF.

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