Elemental mapping using PIXE shows the main pathway of nickel movement is principally symplastic within the fruit of the hyperaccumulator <i>Stackhousia tryonii</i>

Bhatia, Naveen P.; Orlić, I.; Siegele, Rainer; Ashwath, Nanjappa; Baker, A. J. M.; Walsh, Kerry B.

doi:10.1046/j.1469-8137.2003.00912.x

Cited by 46 publications

(48 citation statements)

References 35 publications

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“…These types of X-ray spectrometry techniques are increasingly used because classical analysis require expensive equipment, highly trained analysts, contamination free reagents and extensive sample preparation (Vogel-Mikuš et al 2007). For example, micro-PIXE technique has been employed for elemental localization within fruits, seeds and vegetative tissues of metal hyperaccumulating plants (MesjaszPrzybyowicz et al 1997(MesjaszPrzybyowicz et al , 1999Bhatia et al 2003Bhatia et al , 2004Przybyłowicz et al 2004).…”

Section: Distribution Of Elements In Seedsmentioning

confidence: 99%

Effects of soil contamination by trace elements on white poplar progeny: seed germination and seedling vigour

Madejón

Cantos

Jiménez-Ramos

et al. 2015

Environ Monit Assess

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Seed germination is considered a critical phase in plant development and relatively sensitive to heavy metals. White poplar (Populus alba) trees tend to accumulate Cd and Zn in their tissues. We tested if soil contamination can affect P. alba progeny, reduced seed germination and explored the distribution of mineral elements in the seed. For this purpose, fruits and seeds from female P. alba trees were selected from two contaminated and one non-contaminated areas. Seeds from all the sites were germinated using only water or a nutritive solution (in vitro). Concentrations of nutrients and trace elements in the fruits and seeds were analysed. Seedling growth in vitro was also analysed. Finally, a mapping of different elements within the poplar seed was obtained by particle-induced X-ray emission (PIXE). Germination was similar between different progenies, refuting our hypothesis that seeds from a contaminated origin would have reduced germination capacity compared to those from a non-contaminated site. Seedling growth was not affected by the contaminated origin. Cadmium and Zn concentrations in fruits produced by P. alba trees in the contaminated sites were higher than by those from the non-contaminated site. However, the nutritional status of the trees was adequate in both cases. Cd in seedlings was higher in those from contaminated soils although lower than in fruits, indicating a certain exclusion from seeds. Preliminary results of the PIXE technique showed that Al and Zn were distributed uniformly in the seeds (Cd was not detected with this technique), while the nutrients P and S were concentrated in the cotyledons.

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Section: Distribution Of Elements In Seedsmentioning

confidence: 99%

Effects of soil contamination by trace elements on white poplar progeny: seed germination and seedling vigour

Madejón

Cantos

Jiménez-Ramos

et al. 2015

Environ Monit Assess

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“…However, micro-PIXE localization of Cd in leaves of T. praecox showed substantial concentrations of Cd in phloem (up to 2,000 μg g −1 DW) (Vogel-Mikuš et al 2008a), and that Cd can be accumulated to a great extent in the seeds of T. praecox (up to 1,350 μg g −1 DW) without drastically affecting seed viability . It is generally believed that heavy metals in seeds are localized mainly in the seed coat, which protects the embryo from the metal burden (Ernst et al 2000;Bhatia et al 2003). In T. praecox, on the contrary, Cd is mainly accumulated in the embryonic tissues, namely the epidermis of cotyledons (1,800 μg g −1 DW), with substantial concentrations also in the cotyledon mesophyll (780 μg g −1 DW) and in the radicle (550 μg g −1 DW).…”

Section: Introductionmentioning

confidence: 99%

Complexation of cadmium in seeds and vegetative tissues of the cadmium hyperaccumulator Thlaspi praecox as studied by X-ray absorption spectroscopy

2010

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The cadmium hyperaccumulator Thlaspi praecox Wulfen (Brassicaceae) can accumulate unusually high amounts of Cd (>1,000 μg g −1 dry weight) in its seeds without drastically affecting seed viability. As embryonic tissues are the most sensitive to Cd toxicity, the aim of this study was to investigate the Cd coordination and ligand environment in seeds of field collected T. praecox using extended X-ray absorption fine structure (EXAFS), and to compare the Cd ligand environment to that in the vegetative tissues of the plant. In intact seeds and isolated embryos, almost two thirds of the Cd ligands were thiol groups (Cd-S-C-).In addition, there was coordination to phosphate groups via bridging oxygens (Cd-O-P-), as for phytate, although this ligand was not observed in the vegetative organs and tissues. In roots and shoots up to 80% of the Cd ligands were oxygen ligands that are provided by the cell walls and by organic acids stored in vacuoles. In leaf epidermis only a slightly higher percentage of oxygen ligands was detected, as compared to the mesophyll, making vacuolar compartmentation and binding to the cell walls the main detoxification mechanisms in both of these leaf tissues.

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“…The study of metal ion homeostasis in plants can benefit from spatially resolved metal analysis techniques, such as particle-induced x-ray emission (Bhatia et al, 2003;Isaure et al, 2006) and nanosecondary ion mass spectroscopy (Moore et al, 2010;Smart et al, 2010). Synchrotron x-ray fluorescence (SXRF) microscopy has been used to characterize gene function in plants (Kim et al, 2006).…”

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confidence: 99%

The Role of CAX1 and CAX3 in Elemental Distribution and Abundance in Arabidopsis Seed

et al. 2012

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The ability to alter nutrient partitioning within plants cells is poorly understood. In Arabidopsis (Arabidopsis thaliana), a family of endomembrane cation exchangers (CAXs) transports Ca 2+ and other cations. However, experiments have not focused on how the distribution and partitioning of calcium (Ca) and other elements within seeds are altered by perturbed CAX activity. Here, we investigate Ca distribution and abundance in Arabidopsis seed from cax1 and cax3 loss-of-function lines and lines expressing deregulated CAX1 using synchrotron x-ray fluorescence microscopy. We conducted 7-to 10-mm resolution in vivo x-ray microtomography on dry mature seed and 0.2-mm resolution x-ray microscopy on embryos from lines overexpressing deregulated CAX1 (35S-sCAX1) and cax1cax3 double mutants only. Tomograms showed an increased concentration of Ca in both the seed coat and the embryo in cax1, cax3, and cax1cax3 lines compared with the wild type. High-resolution elemental images of the mutants showed that perturbed CAX activity altered Ca partitioning within cells, reducing Ca partitioning into organelles and/or increasing Ca in the cytosol and abolishing tissue-level Ca gradients. In comparison with traditional volume-averaged metal analysis, which confirmed subtle changes in seed elemental composition, the collection of spatially resolved data at varying resolutions provides insight into the impact of altered CAX activity on seed metal distribution and indicates a cell type-specific function of CAX1 and CAX3 in partitioning Ca into organelles. This work highlights a powerful technology for inferring transport function and quantifying nutrient changes.

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Elemental mapping using PIXE shows the main pathway of nickel movement is principally symplastic within the fruit of the hyperaccumulator Stackhousia tryonii

Cited by 46 publications

References 35 publications

Effects of soil contamination by trace elements on white poplar progeny: seed germination and seedling vigour

Effects of soil contamination by trace elements on white poplar progeny: seed germination and seedling vigour

Complexation of cadmium in seeds and vegetative tissues of the cadmium hyperaccumulator Thlaspi praecox as studied by X-ray absorption spectroscopy

The Role of CAX1 and CAX3 in Elemental Distribution and Abundance in Arabidopsis Seed

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