Elemental mass spectrometry for quantitative proteomics

Sanz‐Medel, Alfredo; Montes-Bayón, Marı́a; Campa, María del Rosario Fernández de la; Encinar, Jorge Ruiz; Bettmer, Jörg

doi:10.1007/s00216-007-1615-2

Cited by 125 publications

(103 citation statements)

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“…This permits the indirect quantification of the SOD concentration provided that the metal/protein stoichiometry is maintained along the whole procedure (input quantities for the calculation are given in the ESI †). 24,25 The concentration obtained was 0.17 AE 0.01 mg mL À1 of Cu (2.7 mM) (n ¼ 3 injections of the diluted synthesized standard) for the isotopically enriched SOD1. Metal concentrations can be converted into protein concentrations since the stoichiometry of the proteinmetal complex is known for both metals (ratio of 2 : 1 metal per protein).…”

Section: Species-specific Isotope Dilution For Human Sod1 Analysismentioning

confidence: 99%

“…However, this is a time consuming and expensive methodology since spiking at 2-3 levels of concentration have to be conducted. 25 Another possibility is the use of an adequate internal standard. This possibility has been demonstrated successfully for quantification using ICP-MS and the capability of this instrument to provide almost species independent ionization of heteroelements.…”

Section: Introductionmentioning

confidence: 99%

“…26 Indeed, the best results should be obtained if the internal standard were as close in structure as possible to the sought molecule and if it can be spiked at the beginning of the sample preparation. 25 This is approximated in protein analysis by using bovine standards which are often available and relatively affordable. 27 Thus, in this work we explored the possibility to conduct the determination of Cu, Zn-SOD in human red blood cells by using a bovine Cu, Zn-SOD as internal standard.…”

Section: Introductionmentioning

confidence: 99%

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Species specific isotope dilution versus internal standardization strategies for the determination of Cu, Zn-superoxide dismutase in red blood cells

Ordóñez

Deitrich

Montes-Bayón

et al. 2011

J. Anal. At. Spectrom.

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Many proteins (more than one third) contain metal ions either within their own structures or bound to some of their active sites. These metals are involved in numerous biological processes and therefore, the quantification of metalloproteins that can serve as clinical biomarkers is of great interest. With this aim, the development of analytical strategies that permit individual (targeted) protein quantitative analysis is attempted in this work. In particular, the evaluation of different strategies for the determination of Cu, Zn-superoxide dismutase (Cu, Zn-SOD), a metalloprotein present in the first-line antioxidant defence system of the body, is conducted. The first analytical strategy is based on the use of bovine Cu, Zn-SOD as internal standard for the quantitative analysis of human Cu, Zn-SOD. For this aim, the chromatographic separation between both species (bovine and human) has been optimized according to their respective isoelectric point by anion exchange chromatography. Interestingly, the obtained results revealed a faster specific degradation of the bovine standard with respect to human SOD during sample preparation. The second strategy involves the production and evaluation of an isotopically enriched metalloprotein standard to be used as tracer in the species specific isotope dilution (SS-IDA) method by measuring the Cu associated to the protein. This is done by liquid chromatography with online inductively coupled plasma mass spectrometric (ICP-MS) detection and applied to the quantification in bovine erythrocytes. This finding is a good example to illustrate the power of SS-IDA for targeted protein quantification in respect to the commonly used alternative standards.

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Section: Species-specific Isotope Dilution For Human Sod1 Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Species specific isotope dilution versus internal standardization strategies for the determination of Cu, Zn-superoxide dismutase in red blood cells

Ordóñez

Deitrich

Montes-Bayón

et al. 2011

J. Anal. At. Spectrom.

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“…This is another challenge being increasingly addressed in recent years. [54][55][56][57] In this sequence, two possibilities are described to perform online spike for ID quantification of species by ICP-MS. The basis for quantification of species by ID in flow systems involving different separation techniques coupled to ICP-MS is presented.…”

Section: Id Modes For Speciation Analysismentioning

confidence: 99%

Online isotope dilution and inductively coupled plasma mass spectrometry: from elemental to species quantification

Giné¹,

Packer²

2010

J. Braz. Chem. Soc.

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Apresentam-se sistemas de análises em fluxo propostos para a quantificação de elementos e espécies por diluição isotópica (ID) utilizando espectrometria de massas com fonte de plasma acoplada indutivamente (ICP-MS). Detalham-se os esquemas para adicionar em linha a solução isotopicamente enriquecida à amostra. São relatadas as aplicações em meio aquoso e outras com transferência do analito para a fase vapor, seja por processo de vaporização eletrotérmica, geração de vapor ou ablação por laser. Apresentam-se as aplicações com quantificação de espécies por ID caracterizadas pela adição do isótopo enriquecido, antes ou após a separação das espécies, seja por cromatografia a líquido, a gás ou por eletroforese capilar acoplada a ICP-MS. As principais equações envolvidas na quantificação por ID e as principais fontes de erro são descritas. São discutidas as mudanças introduzidas na estratégia de quantificação temporal por ID de elementos e espécies pela calibração do fluxo de massas da amostra e do isótopo enriquecido adicionado.Flow analysis has been utilized for elemental and species quantification by online isotope dilution inductively coupled plasma mass spectrometry (ICP-MS). Online isotope dilution (ID) herein refers to strategies promoting the addition of enriched isotopic solutions to the samples while flowing towards the plasma. Applications of online ID's are commonly performed in aqueous media, however, in some processes the analyte transference to the vapor phase using electrothermal vaporization, vapor generation or laser ablation is also required. Flow diagrams to perform online pre or post-column spiking with the enriched isotope for quantification by ID in speciation analysis by liquid chromatography, gas chromatography or capillary electrophoresis devices coupled to ICP-MS are presented. The main figures of merit and equations applied to elemental and species quantification by ID in flowing schemes are assessed.

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“…Applications of ICP-MS to biomolecules have been especially boosted by the present commercial availability of collision and reaction cells and high-resolution (doublefocusing) ICP-MS instrumentation, as well as by the development of suitable interfaces for coupling of ICP-MS with separation techniques [104]. Moreover, recently, ICP-MS has been demonstrated to be a highly valuable tool for ultrasensitive detection and characterisation of metalloid-containing NPs.…”

Section: Inductively Coupled Plasma Mass Spectrometry For Detection mentioning

confidence: 99%

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

et al. 2009

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Inorganic mass spectrometry techniques may offer great potential for the characterisation at the nanoscale, because they provide unique elemental information of great value for a better understanding of processes occurring at nanometre-length dimensions. Two main groups of techniques are reviewed: those allowing direct solid analysis with spatial resolution capabilities, i.e. lateral (imaging) and/or indepth profile, and those for the analysis of liquids containing colloids. In this context, the present capabilities of widespread elemental mass spectrometry techniques such as laser ablation coupled with inductively coupled plasma mass spectrometry (ICP-MS), glow discharge mass spectrometry and secondary ion/neutral mass spectrometry are described and compared through selected examples from various scientific fields. On the other hand, approaches for the characterisation (i.e. size, composition, presence of impurities, etc.) of colloidal solutions containing nanoparticles by the well-established ICP-MS technique are described. In this latter case, the capabilities derived from the on-line coupling of separation techniques such as field-flow fractionation and liquid chromatography with ICP-MS are also assessed. Finally, appealing trends using ICP-MS for bioassays with biomolecules labelled with nanoparticles are delineated.

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Elemental mass spectrometry for quantitative proteomics

Cited by 125 publications

References 100 publications

Species specific isotope dilution versus internal standardization strategies for the determination of Cu, Zn-superoxide dismutase in red blood cells

Species specific isotope dilution versus internal standardization strategies for the determination of Cu, Zn-superoxide dismutase in red blood cells

Online isotope dilution and inductively coupled plasma mass spectrometry: from elemental to species quantification

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

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