Elemental, Nano-Sized (100-500 nm) Selenium Production by Probiotic Lactic Acid Bacteria

Abstract

Abstract-Selenium is well known as an essential trace element since the 20th century, but it can be overdosed easily because of its toxicity. According to the present regulations [1] only the potentially most harmful, inorganic selenium salts can be added to any comestibles. Elemental selenium is considered as the least toxical of all selenium forms and in the same time supplementation with its nano-size particles has the same or better bioavailability compared to its salts. In our experiments we managed to produce nano-size (100-500nm) elemental selenium by using probiotic yogurt bacteria in a fermentation procedure. We developed the laboratory technology of product purification and recovery from bacteria.

Bacillus cereus, selenite-reducing bacterium from contaminated lake of an industrial area: a renewable nanofactory for the synthesis of selenium nanoparticles

“…These results are compared with earlier study carried out on biosynthesis of selenium nanoparticles with marine bacterial strain Bacillus sp. MSh-1, in which nanoparticles in size range of about 80-220 nm were obtained (Forootanfar et al 2014), while the probiotic lactic acid bacteria such as Lactobacillus and Bifidobacter produced selenium nanoparticles in the size ranges of 100-200 and 400-500 nm, respectively (Eszenyi et al 2011). From the data, it is evident that the strain B. cereus is able to produce selenium nanoparticles with smaller average nanoparticle size and narrow particle size distribution.…”

“…(Bajaj et al 2012), Zooglea ramigera (Srivastava and Mukhopadhyay 2013), Streptomyces minutiscleroticus M10A62 (Ramya et al 2015), Acinetobacter sp. E6.2 (Durán et al 2015), Comamonas testosteroni S44 (Zheng et al 2014), Klebsiella pneumonia (Fesharaki et al 2010), Lactobacillus sp, Bifidobacter sp., and Streptococcus thermophilus (Eszenyi et al 2011).…”

Selenite-stress selected mutant strains of probiotic bacteria for Se source production

“…casei was reported to accumulate 400 mg Se kg −1 274 dry biomass, and at least 80% of it was associated with organic 275 molecules like selenocysteine [20]. However, the L. casei 431 strain 276 was also described as a potential nanoselenium producer probiotic 277 organism, which produced 1500-3500 mg Se kg −1 dry biomass in 278 the presence of 200 mg L −1 NaHSeO 3 (1.32 mmol L −1 Se) at 37 • C 279 [21]. In our experiments, the mutant of L. casei 431 strain accumu-280 lated about 10 times more total selenium (37,428 ± 978 mg kg −1 281 dry biomass).…”

Selenium Enriched Probiotic Products With Functional Properties

“…С. [6][7][8][9][10][11][12][13][14][15][16][17][18] Известно, что пробиотические микроорганизмы способны накапливать и биотрансформировать селен при внесении его в среду культивирования. До-бавление селена улучшает их окислительно-восстановительный потенциал, за счет образования органических форм селена [6].…”

“…С. [6][7][8][9][10][11][12][13][14][15][16][17][18] маргарины и безалкогольные напитки, специальные пищевые продукты. Значительный удельный вес (65-70%) приходится на долю молочных продук-тов.…”