Elementos de transposição: diversidade, evolução, aplicações e impacto nos genomas dos seres vivos

Carareto, Cláudia Márcia Aparecida; Monteiro-Vitorello, Cláudia Barros

doi:10.7476/9788575415672

Cited by 1 publication

(6 citation statements)

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“…LTR retrotransposons are the most abundant elements in the genome of plants, possibly because they perform their transposition mediated by mRNA through a replication mechanism, known as “copy and paste”, thus increasing the size of the genome [18, 20]. The long repetitive sequences present at the endings of the retrotransposons do not encode polypeptide but play an important role in the regulation of retroelements [21].…”

Section: Discussionmentioning

confidence: 99%

“…However, in the Eleocharis genome was found greater predominance of Ty1/Copy compared to Ty3/Gypsy [53]. The main difference between superfamilies Ty3/Gypsy and Ty1/Copy is in the structural organization of their intermediate polyprotein molecule, and both are the most frequent and diversified forms in the genomes of eukaryotes [16, 21].…”

Section: Discussionmentioning

confidence: 99%

“…Satellite DNAs are composed by highly repetitive sequences in tandem , and are generally located in heterochromatin regions (found mainly in centromeric or subtelomeric regions) [8, 21]. The SatDNA hybridization of PeSat_1 (CL 207) was expected in the centromeric region, due to its characterization as SatDNA, circular graph and hits with similarities to centromere DNA in other species.…”

Section: Discussionmentioning

confidence: 99%

“…LTR retrotransposons comprise a group of repetitive DNAs in the genome of plants, with sequence sizes varying between 300 to 5000 nucleotides [21]. The CLs of P. edulis , classified as LTRs, have not represented the entire element, and the sizes observed ranged from 411 to 2274 nucleotides (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Our study focused on LTR-retrotransposon, which is characterized by an internal region formed of gag genes ( group-specific antigen , encoding structural proteins similar to the viral capsid), pol genes ( polyprotein , encoding the catalytic protein integrase (INT)), reverse transcriptase (RT) and RNAseH (RH, required for replication), and long terminal repeats [20]. The Ty1/Copy superfamily is the most abundant LTR retrotransposons within plant genomes, differing in the preserved domain of RT genes and in the position of the INT domain within the pol complex [21].…”

Section: Introductionmentioning

confidence: 99%

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Low coverage sequencing for repetitive DNA analysis in Passiflora edulis Sims: citogenomic characterization of transposable elements and satellite DNA

et al. 2019

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Background The cytogenomic study of repetitive regions is fundamental for the understanding of morphofunctional mechanisms and genome evolution. Passiflora edulis a species of relevant agronomic value, this work had its genome sequenced by next generation sequencing and bioinformatics analysis performed by RepeatExplorer pipeline. The clusters allowed the identification and characterization of repetitive elements (predominant contributors to most plant genomes). The aim of this study was to identify, characterize and map the repetitive DNA of P. edulis , providing important cytogenomic markers, especially sequences associated with the centromere. Results Three clusters of satellite DNAs (69, 118 and 207) and seven clusters of Long Terminal Repeat (LTR) retrotransposons of the superfamilies Ty1/Copy and Ty3/Gypsy and families Angela, Athila, Chromovirus and Maximus-Sire (6, 11, 36, 43, 86, 94 and 135) were characterized and analyzed. The chromosome mapping of satellite DNAs showed two hybridization sites co-located in the 5S rDNA region (PeSat_1), subterminal hybridizations (PeSat_3) and hybridization in four sites, co-located in the 45S rDNA region (PeSat_2). Most of the retroelements hybridizations showed signals scattered in the chromosomes, diverging in abundance, and only the cluster 6 presented pericentromeric regions marking. No satellite DNAs and retroelement associated with centromere was observed. Conclusion P. edulis has a highly repetitive genome, with the predominance of Ty3/Gypsy LTR retrotransposon. The satellite DNAs and LTR retrotransposon characterized are promising markers for investigation of the evolutionary patterns and genetic distinction of species and hybrids of Passiflora .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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