Elements of water bacteriology, with special reference to sanitary water analysis

Prescott, S. C.; Winslow, C.-E. A.

doi:10.5962/bhl.title.100720

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Assessing the microbial quality of drinking water by means of bacterial cell culture is well established and has been applied since the early 1900s [ 5 ]. The major technological changes have been the introduction of quantification by multiple tube fermentation, development of membrane filtration and more recently enzyme substrates [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

et al. 2015

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The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in low-income countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti-Tray®. AT was compared with IDEXX Colilert-18® and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-18® for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely.

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confidence: 99%

Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

et al. 2015

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“…The use of Clostridiumperfringens as a water quality indicator has been a subject of considerable controversy (3,21,23,26) since 1899 when Klein and Houston (15) first suggested that this organism could be used to detect fecal pollution. It is rarely used as such in the United States, for the reasons stated by Levine (17) and summarized by Prescott et al (21). However, a number of European workers (23, 26) continued to recommend C. perfringens as a valuable supplement to other water quality tests, particularly in certain specific situations.…”

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Membrane filter enumeration method for Clostridium perfringens

Bisson¹,

Cabelli²

1979

Appl Environ Microbiol

147

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A membrane filter procedure has been developed for the rapid quantitation of C. perfringens in the aquatic environment. Background growth is inhibited by the use of D-cycloserine, polymyxin B sulfate, and incubation at 450C. Differential characteristics include the fermentation of sucrose, production of acid phosphatase, and the absence of ,B-D-glucosidase activity. The medium is prepared as follows (in grams per 100 ml of distilled water): tryptose, 3.0; yeast extract, 2.0; sucrose, 0.5; L-cysteine, 0.1; MgSO4.7H20, 0.01; bromocresol purple, 0.004; and agar, 1.5. The ingredients are dissolved, and the pH is adjusted to 7.6. After autoclaving at 1210C for 15 min, the medium is allowed to cool at 500C, and the following are added per 100 ml: D-cycloserine, 40 mg; polymyxin B sulfate, 2.5 mg; indoxyl-fl-D-glucoside, 60 mg, 2.0 ml of a filter-sterilized 0.5% phenolpthalein diphosphate solution; and 0.2 ml of a filter-sterilized 4.5% FeCl3-6H20 solution. Enumeration of C. perfringens in a water sample is completed within 18 to 24 h. The verification of typical colonies was 93%. The average recovery from peptonewater spore suspensions of five strains was 79%, and that from filter-sterilized seawater suspensions was 90%. The precision of the method was approximately equal to that expected from random error alone. Confirmed recoveries of C. perfringens from water and sewage samples generally were greater than those by the Bonde pour tube method.

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Elements of water bacteriology, with special reference to sanitary water analysis

Cited by 2 publications

References 11 publications

Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

Membrane filter enumeration method for Clostridium perfringens

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