Eletrophysiological study of the caudal nerve on developing rats

Maia, Juliana Netto; Carvalho, Celina Cordeiro de; Galvão, Marina Hazin; Silva, Anderson de Lima; Mendes, Ana Carine Gouveia; Moraes, Sílvia Regina Arruda de; Lins, Otávio Gomes

doi:10.1590/s0102-86502010000200004

Cited by 8 publications

(6 citation statements)

References 9 publications

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“…To investigate this issue, we examined Clec16a ΔUBC mice that had reached a disability score of 4, 24 days after initiating tamoxifen treatment, along with age-matched control/Clec16a loxP/loxP mice. We measured the compound action potentials (CAPs) of the tail, which is a mixed nerve response from the bilateral dorsal and ventral caudal nerves, mainly comprised of myelinated and unmyelinated sensory axons [25][26][27] . Compared to control/Clec16a loxP/loxP mice, Clec16a ΔUBC mice had a profoundly smaller CAP amplitude and a mildly decreased conduction velocity (Fig.…”

Section: Resultsmentioning

confidence: 99%

Inducible knockout of Clec16a in mice results in sensory neurodegeneration

Hain

Pandey

Bakay

et al. 2021

Sci Rep

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CLEC16A has been shown to play a role in autophagy/mitophagy processes. Additionally, genetic variants in CLEC16A have been implicated in multiple autoimmune diseases. We generated an inducible whole-body knockout, Clec16aΔUBC mice, to investigate the loss of function of CLEC16A. The mice exhibited a neuronal phenotype including tremors and impaired gait that rapidly progressed to dystonic postures. Nerve conduction studies and pathological analysis revealed loss of sensory axons that are associated with this phenotype. Activated microglia and astrocytes were found in regions of the CNS. Several mitochondrial-related proteins were up- or down-regulated. Upregulation of interferon stimulated gene 15 (IGS15) were observed in neuronal tissues. CLEC16A expression inversely related to IGS15 expression. ISG15 may be the link between CLEC16A and downstream autoimmune, inflammatory processes. Our results demonstrate that a whole-body, inducible knockout of Clec16a in mice results in an inflammatory neurodegenerative phenotype resembling spinocerebellar ataxia.

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Section: Resultsmentioning

confidence: 99%

Inducible knockout of Clec16a in mice results in sensory neurodegeneration

Hain

Pandey

Bakay

et al. 2021

Sci Rep

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“…The possibility of comparing the obtained data was feasible due to the standardization of weights and sex of the animals 2 , 5 , 7 , 13 and the constant calibration and maintenance of temperature above the levels described by Stecker & Baylor 4 , in a study of the effect of temperature on the potential of nervous action, in which only there were alterations of amplitude and area when the animals were submitted to temperatures below 27ºC, and permanent loss of the nervous action potential appeared only after cooling below 10ºC for long periods.…”

Section: Discussionmentioning

confidence: 99%

Does the type of electrode affect the electromyoneurographic parameters in rats?

et al. 2019

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Purpose To evaluate if the type of electrode (needle vs. surface) affects the electromyoneurography parameters in rats. Methods Twenty male rats were anesthetized, then compound muscle action potential were recorded using a Neuropack S1 MEB- 9400 © . All animals were submitted to two electroneuromyography analysis: first with surface electrode and then by needle electrode. We evaluated the latency, amplitude, duration and area of the negative peak of the gastrocnemius and cranial tibial muscles. Results There were no significant differences between the groups in the mean of duration, latency, amplitude or area of the negative peak in gastrocnemius and cranial tibial muscles. Conclusion The type of electrode does not affect the electroneuromyography parameters.

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“…After anesthesia with a intramuscular injection of a mixture of Xylazin Chlorhydrate (Rompum) and Ketamin (Ketalar), 0.2ml/100g 19 , animals were positioned ventrally for their tail to be completely loose and the tail was clean and fat free with alcohol at 70%.…”

Section: Electrophysiologymentioning

confidence: 99%

“…An orthodromic technique was used 19 : The caudal nerve was stimulated distally (duration: 0.1ms; intensity: 10-20µA, supramaximal) and the sensory nerve action potentials (SNAP's) were recorded proximally (high-pass filter: 10Hz; low-pass filter: 10kHz) on the animal's tail. No movement of the tail was noted during stimulation.…”

Section: Electrophysiologymentioning

confidence: 99%

“…Measurements were taken 4 times and the accepted result was their arithmetic average. Stimulation, recording and ground electrodes were identical standard "alligator" clips 19 . The distance between the stimulating cathode and the non-inverting recording electrode was 8cm, whereas the distance between the pair of stimulating and recording electrodes was 3cm.…”

Section: Electrophysiologymentioning

confidence: 99%

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Sensory nerve conduction in the caudal nerves of rats with diabetes

Carvalho¹,

Maia²,

Lins³

et al. 2011

Acta Cir. Bras.

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PURPOSE: To investigate sensory nerve conduction of the caudal nerve in normal and diabetic rats. METHODS: Diabetes was induced in twenty 8-weeks old Wistar male rats. Twenty normal rats served as controls. Caudal nerve conduction studies were made before diabetes induction and the end of each week for six consecutive weeks. The caudal nerve was stimulated distally and nerve potentials were recorded proximally on the animal's tail using common "alligator" clips as surface electrodes. RESULTS: After induction, nerve conduction velocities (NCV) increased slower in the diabetic than in the control group. Sensory nerve action potentials (SNAP) conduction velocities increased slower in the diabetic than in the control group (slope of regression line: 0.5 vs 1.3m/s per week; NCV in the 15th week = 39±3m/s vs 44±4m/s). Tukey's tests showed differences between groups at the 11th, 13th and 15th weeks old. From the 10th week on, SNAP amplitudes increased faster in the diabetic than in the control group (slopes of the regression line: 10 vs 8µV per week; SNAP amplitudes in the 15th week: 107±23µV vs 85±13µV). Differences at the 12th, 13th and 15th weeks were significant. CONCLUSION: In diabetic rats nerve conduction velocities were slower whereas amplitudes were larger than in normal rats.

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Eletrophysiological study of the caudal nerve on developing rats

Abstract: It was possible to study non-invasively the sensitive conduction of the caudal nerve of normal developing rats and describe reference values. The technique and data may be used as animal model in physiological and pathological studies.

Cited by 8 publications

References 9 publications

Inducible knockout of Clec16a in mice results in sensory neurodegeneration

Inducible knockout of Clec16a in mice results in sensory neurodegeneration

Does the type of electrode affect the electromyoneurographic parameters in rats?

Sensory nerve conduction in the caudal nerves of rats with diabetes

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