Eletropolymerization of Niacinamide for Fabrication of Electrochemical Sensor: Simultaneous Determination of Dopamine, Uric Acid and Ascorbic Acid

Abstract

Two polymeric thin film modified electrodes, poly-niacinamide/glassy carbon electrode (poly-NA/GCE) and poly-nicotinic acid/glassy carbon electrode (poly-NC/GCE), have be fabricated at glassy carbon electrodes by simple electropolymerization of niacinamide (NA) in different potential scan ranges of cyclic voltammetry (CV). These two electrodes all showed catalytic ability towards the oxidation of dopamine (DA), uric acid (UA) and ascorbic acid (AA) by obvious reductions of overpotentials, giving well-resolved voltammetric peaks, which allow simultaneously determination of DA, UA and AA. The poly-NC/GCE showed even higher electrocatalytic activity than the poly-NA/GCE. The electrochemical behavior of poly-NC/GCE was investigated by CV and differential pulse voltammetry (DPV) for determination of DA, UA and AA. The linear ranges of the concentration for the determination of AA, DA and UA using DPV were 75-3000, 0.37-16 and 0.741-230 µmol•L -1 , respectively. The slopes of the linear calibration curves for the detection were estimated to be 5.6, 1140 and 102 mA•L•mol -1 for AA, DA and UA, respectively. The poly-NC/GCE shows excellent sensitivity, good selectivity and antifouling properties.

Voltammetric microsensor using PEDOT-modified gold electrode for the simultaneous assay of ascorbic and uric acids

“…Several electrode modification processes have been tested, using highly oxidized metal electrodes [16], metal electrodeposition [17], metal complexes [18,19], electrochemical reduction of diazonium salts [20], mesoporous silica [21], single [22] or multiwalled carbon nanotubes [23], self-assembled thiol monolayer [24], ion-exchange Nafion membrane [25] or conducting polymers like poly vinyl alcohol [26], polypyrrole [27] or luminol [28] for measurements in rat brain [29] or in human urine and serum samples [30]. All these studies allowed the simultaneous detection and determination of AA and UA under similar concentrations [31][32][33] or the assay of UA in samples containing a large excess of AA [34][35][36]. The analytical performances of these modified electrodes are listed in Table 1.…”

Evidence of an EC’ mechanism occurring during the simultaneous assay of ascorbic and uric acids on poly(3,4-ethylenedioxythiophene) modified gold microsensor

“…However a very few number of these studies focused on the simultaneous detection and determination of AA and UA under similar concentrations [13][14][15]. For the greatest part of these papers, two situations are generally studied: the first considers AA and/or UA as interfering species, particularly for the assay of dopamine [16][17][18][19]; in the second condition, the assay of UA is performed in samples containing AA in large excess [20][21][22][23]. However these conditions do not reflect the healthy human situation: the concentration of AA in blood serum is one order of magnitude lower than that of UA (34-79 μM and 180-420 μM respectively [24,25]).…”

Photoisomerization of electroactive polyimide/multiwalled carbon nanotube composites on the effect of electrochemical sensing for ascorbic acid

“…. The differential pulse voltammetry (DPV) data of 20 µmol L −1 AA, 60 µmol L −1 DA and 120 µmol L −1 UA mixtures in PBS (pH 7.0) show well‐resolved anodic peaks at −0.03, 0.13 and 0.38 V, respectively . The oxidation of DA and UA does not influence the current response of AA.…”