Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model

Abstract

It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive compounds that have the ability as chemopreventive agents. This study aims to examine the effect of Eleutherine palmifolia (L.) Merr extract (EPE) on colon histopathology and the enhancement of caspase-3 expression in BALB/c mice of colitis-associated colon cancer (CAC) model. Thirty Balb/c female mice were used in this study and were divided into six groups. Five mice in one group were normal or negative control (given phosphate-buffered saline), and twenty-five mice were induced intraperitoneally with 10 mg/kg BW AOM (Azoxymethane), and it was followed by the administration of 5% DSS (Dextran Sodium Sulfate) every two weeks for 20 weeks. At the sixth week, one mice in each group was sacrificed for the Fecal Occult Bold Test (FOBT) and serum amyloid α (SAA) test to ensure that CAC was indeed formed. The administration of EPE with varying doses was started from the eighth week and was continued until the 21st week. The length of the colonic crypt was measured through histology appearance using Hematoxylin-eosin (HE) staining while the immunohistochemical method was used to observe apoptotic activity through the enhancement of caspase-3 expression. The results showed that the increase in EPE dosage also increased the crypt colon length compared to the positive control group. The administration of 1.00 mg/20gBW EPE significantly increased cell apoptosis which can be observed through caspase-3 expression compared to the positive control group (p<0.05). Based on these data, the extract of EPE can be developed as phytotherapy for chemopreventive.
 

Effect of 1,4-naphthoquinone from Sisyrinchium palmifolium L. extract on in vivo Ki-67 expression and in silico CDK1, CDK2, CDK4 on colitis-associated colon cancer

“…Based on research, it shows that the ethanol extract of S. palmifolium can inhibit the growth of HeLa cervical cancer cells (Muti'ah et al, 2018). Another study showed that the administration of ethanol extract of S. palmifolium could trigger the apoptotic activity of cancer cells both intrinsically and extrinsically (Muti'ah et al, 2020). This is supported by previous studies, which showed that S. palmifolium could function as colonic anti-cancer agents (Li et al, 2008).…”

Advances in Quantitative and Qualitative Assessment of Collagen in Cutaneous Wound Healing

“…One of the most common methods to analyze various tissue healing or pathology at the microscopic structural level is hematoxylin eosin staining (Nurrahma et al, 2022;Mutiah et al, 2020, Wardani et al, 2022Pananchery & Gadgoli, 2021). Histopathological staining using hematoxylin-eosin (HE) is one of the most common analytical methods.…”