Eleutheroside B1 mediates its anti-influenza activity through POLR2A and N-glycosylation

Wen, Yan; Zheng, Chunge; He, Jiayang; Zhang, Wenjie; Huang, Xinan; Li, Xiong; Wang, Yutao; Wang, Xinhua

doi:10.3892/ijmm.2018.3863

Cited by 12 publications

(13 citation statements)

References 51 publications

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“…This combination was tested in controlled clinical trials for the treatment of common cold and influenza‐associated uncomplicated upper respiratory infections as well as for the prevention of common colds 421–424 . The studies confirmed the safety and superior efficacy of this combination regimen as compared with monodrug therapy, 425 presumably because of its antiviral effects, 426–432 effects on innate and adaptive immunity, 433–437 and anti‐inflammatory, antioxidant, and detoxifying effects 438–441 of both adaptogenic plants as well as due to their synery 25 . It should be noted that the postmarketing pharmacovigilance assessment of Kan Jang showed a high benefit–risk ratio: one adverse event in about 100,000 patients was recorded for the 23‐year period from the adverse event reports (concerning mainly allergic reactions) to the Swedish and Danish medical product agencies.…”

Section: Current and Prospective Use Of Adaptogens In Stress‐induced mentioning

confidence: 84%

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

Panossian¹,

Efferth

Shikov

et al. 2020

Medicinal Research Reviews

209

124

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Adaptogens comprise a category of herbal medicinal and nutritional products promoting adaptability, resilience, and survival of living organisms in stress. The aim of this review was to summarize the growing knowledge about common adaptogenic plants used in various traditional medical systems (TMS) and conventional medicine and to provide a modern rationale for their use in the treatment of stress‐induced and aging‐related disorders. Adaptogens have pharmacologically pleiotropic effects on the neuroendocrine‐immune system, which explain their traditional use for the treatment of a wide range of conditions. They exhibit a biphasic dose‐effect response: at low doses they function as mild stress‐mimetics, which activate the adaptive stress‐response signaling pathways to cope with severe stress. That is in line with their traditional use for preventing premature aging and to maintain good health and vitality. However, the potential of adaptogens remains poorly explored. Treatment of stress and aging‐related diseases require novel approaches. Some combinations of adaptogenic plants provide unique effects due to their synergistic interactions in organisms not obtainable by any ingredient independently. Further progress in this field needs to focus on discovering new combinations of adaptogens based on traditional medical concepts. Robust and rigorous approaches including network pharmacology and systems pharmacology could help in analyzing potential synergistic effects and, more broadly, future uses of adaptogens. In conclusion, the evolution of the adaptogenic concept has led back to basics of TMS and a new level of understanding of holistic approach. It provides a rationale for their use in stress‐induced and aging‐related diseases.

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Section: Current and Prospective Use Of Adaptogens In Stress‐induced mentioning

confidence: 84%

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

Panossian¹,

Efferth

Shikov

et al. 2020

Medicinal Research Reviews

209

124

View full text Add to dashboard Cite

show abstract

“…The formation of subnuclear paraspeckle structures depend on transcription and RNA polymerase II in response to stress or cellular metabolic changes (45). The mRNA data analysis also indicated that the effect of eleutheroside B1 on RNA polymerase and RNA synthesis was required for the anti-influenza activity (46). Previous studies have proven that NEAT1 is a crucial innate immune molecule with dual functions (44).…”

Section: Discussionmentioning

confidence: 98%

“…In addition, another limitation is that ncRNA expression was not examined at different time points. Previous research (46) has often selected 24 h after infection for RNA-seq; therefore, this time point was used for detection in this study. In the future, the authors aim to perform further studies using different time points.…”

Section: Discussionmentioning

confidence: 99%

Effect of eleutheroside B1 on non‑coding RNAs and protein profiles of influenza A virus‑infected A549 cells

et al. 2020

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Influenza viruses often pose a serious threat to animals and human health. In an attempt to explore the potential of herbal medicine as a treatment for influenza virus infection, eleutheroside B1, a coumarin compound extracted from herba sarcandrae, was identified, which exhibited antiviral and anti-inflammatory activities against influenza A virus. In this study, high-throughput RNA sequencing and isobaric tags for relative and absolute quantification (iTRAQ) assays were performed to determine alterations in the non-coding RNA (ncRNA) transcriptome and proteomics. Bioinformatics and target prediction analyses were used to decipher the potential roles of altered ncRNAs in the function of eleutheroside B1. Furthermore, long ncRNA (lncRNA) and mRNA co-expressing networks were constructed to analyze the biological functions by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The analysis of RNA sequencing data revealed that 5 differentially expressed ncRNAs were upregulated and 3 ncRNAs were downregulated in the A549 cells infected with A/PR8/34/H1N1, with or without eleutheroside B1 treatment (PR8+eleu and PR8, respectively). Nuclear paraspeckle assembly transcript 1 (NEAT1) was differentially expressed between the PR8 and A549 cell groups. GO and KEGG pathway analyses indicated that eleutheroside B1 took advantage of the host cell biological processes and molecular function for its antiviral and anti-inflammatory activities, as well as for regulating cytokine-cytokine receptor interaction in the immune system, consistent with previous findings. The results of the iTRAQ assays indicated that L antigen family member 3 (LAGE3) protein, essential for tRNA processing, tRNA metabolic processes and ncRNA processing, was downregulated in the PR8+eleu compared with the PR8 group. In the present study, these comprehensive, large-scale data analysis enhanced the understanding of multiple aspects of the transcriptome and proteomics that are involved in the antiviral and anti-inflammatory activities of eleutheroside B1. These findings demonstrate the potential of eleutheroside B1 for use in the prevention and treatment of influenza A virus-mediated infections.

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“…EP300 and CREBBP target a significant number of proteins for acetylation, including cytosolic proteins involved in essential metabolic processes 21 . POLR2A is a key virus polymerase-interacting protein and is required for viral replication and transcriptional activity 22 . Cell division cycle 20 (CDC20) encodes a regulatory protein and plays important roles in tumorigenesis 23 .…”

Section: Discussionmentioning

confidence: 99%

Identification of potential biomarkers and inhibitors for SARS-CoV-2 infection

Yuan

2020

Preprint

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The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has overwhelmed many health systems globally. Here, we aim to identify biological markers and associated biological processes of COVID-19 using a bioinformatics approach to elucidate their potential pathogenesis. The gene expression profile of the GSE152418 dataset was originally produced by using the high-throughput Illumina NovaSeq 6000. Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and Gene Ontology (GO) enrichment analyses were applied to identify functional categories and biochemical pathways. KEGG and GO results suggested that biological pathways such as Cancer pathways and Insulin pathways were mostly affected in the development of COVID-19. Moreover, we identified several genes including EP300, CREBBP, and POLR2A were involved in the virus activities in COVID-19 patients. We further predicted that some inhibitors may have the potential to block the SARS-CoV-2 infection based on the L1000FWD analysis. Therefore, our study provides further insights into the underlying pathogenesis of COVID-19.

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Eleutheroside B1 mediates its anti-influenza activity through POLR2A and N-glycosylation

Cited by 12 publications

References 51 publications

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

Effect of eleutheroside B1 on non‑coding RNAs and protein profiles of influenza A virus‑infected A549 cells

Identification of potential biomarkers and inhibitors for SARS-CoV-2 infection

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