Elevated dietary intake of Zn-methionate is associated with increased sperm DNA fragmentation in the boar

García-Contreras, Adelfa del Carmen; Loera, Yasmin De; Garcı́a-Artiga, Carlos; Palomo, Antonio; Guevara, Jesús A.; Herrera-Haro, José G.; López‐Fernández, Carmen; Johnston, Susan J; Gosálvez, Jaime

doi:10.1016/j.reprotox.2010.12.003

Cited by 28 publications

(14 citation statements)

References 23 publications

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“…Zinc ions can contribute to the DNA‐protamine structure by linking protamines with zinc bridges, hence stabilizing the chromatin (Björndahl & Kvist, ; Gatewood, Schroth, Schmid, & Bradbury, ). With this in mind, boars used for AI are frequently supplemented with Zn; however, high levels of dietary Zn resulted in increased individual variation in sperm DNA fragmentation (García‐Contreras et al., ). Furthermore, the amount of disulfide bridges in sperm protamines may vary among species and individuals and account for the variability in the SDF (Balhorn, ; Ward & Coffey, ).…”

Section: Discussionmentioning

confidence: 99%

Dynamics of sperm DNA fragmentation in raw boar semen and fertility

Batista

Lier

Petrocelli

2016

Reprod Domestic Animals

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The aims were to evaluate sperm DNA fragmentation (SDF) in boars through the dispersion of their chromatin in raw semen samples, quantifying the extent of SDF, and to assess dynamic aspects of sperm DNA damage after incubation to obtain the rate of sperm DNA fragmentation (rSDF) under thermal conditions similar to the uterus (37°C) over a period of up to 24 hr and to correlate the reproductive outcome of the sows with the SDF of the boars at ejaculation. The study was performed on a pig-breeding farm in southern Uruguay. Sixty-one ejaculates from five of the most frequently used hybrid boars were evaluated. Semen was collected weekly from each of the boars, using the gloved-hand technique and discarding the jelly-like fraction of the ejaculate. Fresh semen was kept in a water bath at 37°C and protected from light, and was thereafter processed with Sperm-Sus-Halomax(®) to evaluate SDF. The smears for time 0 (T0) were made on farm, and thereafter smears were made at the laboratory at 4 hr of obtaining the semen (T4), then every 2 hr (T6, T8, T10, T12) and a final fixation at 24 hr (T24). Differences in SDF were observed among exposure times for all boars (p < .05), but not between T10 and T12 (p = .7751) nor T4 and T24 (p = .9113). In none of the T24 samples, sperm heads could be seen with chromatin dispersion halos. Furthermore, there were differences among boars when comparing sperm rSDF (p < .05). Farrowing rate was not affected by SDF at T0 (r = .38, p = .75), nor was litter size (r = .16, p = .70). With the present experimental conditions, we have not been able to show a relationship between sperm DNA fragmentation at ejaculation and reproductive performance. However, this could be a result of the low number of ejaculates and boars used.

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Section: Discussionmentioning

confidence: 99%

Dynamics of sperm DNA fragmentation in raw boar semen and fertility

Batista

Lier

Petrocelli

2016

Reprod Domestic Animals

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“…Similarly, a considerable percentage of adult mice born as a result of intracytoplasmatic sperm injection (ICSI) using spermatozoa with a high DFI, showed a significant increase in the incidence of abnormal behavior, malformations, tumors, and signs of premature aging [3]. Thus, the quality of the genetic material present in male sperm becomes a prognostic factor for effectiveness of treatment of an infertile couple, ability to bear a healthy child, as well as in determining cancer risk in future generations [4][5][6][7]. Thus, it is important to understand the possible consequences of SDF on embryo growth, implantation, pregnancy outcome, and the health of progeny, conceived naturally or by assisted reproductive technology [8].…”

Section: Introductionmentioning

confidence: 99%

The effect of sperm DNA fragmentation on the dynamics of the embryonic development in intracytoplasmatic sperm injection

Wdowiak

Bakalczuk²,

Bakalczuk

2015

Reproductive Biology

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“…Desde el punto de vista fisiológico el Zn es calificado como un micronutriente crítico, que posee propiedades multifuncionales en el organismo. En el sistema reproductivo del macho, existe en altas concentraciones y participa en el funcionamiento normal de testículos, próstata y epidídimo (García-Contreras et al, 2011). En los testículos, el Zn se encuentra principalmente en las células de Leydig (CL), espermatogonias tipo B, y en las espermátidas (Croxford et al, 2011).…”

Section: Resultados Y Discusiónunclassified

Respuesta al consumo adicional de zinc orgánico en la calidad seminal de ovinos de pelo

et al. 2016

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RESUMEN Con el objetivo de evaluar la respuesta al consumo adicional de diferentes niveles de zinc orgánico en la calidad seminal de ovinos de pelo, se utilizaron 9 borregos prepúberes (entre los 3 ½ a 4 ½ meses de edad) con un peso promedio de 13 kg, los cuales fueron asignados a uno de tres tratamientos en un diseño experimental completamente al azar. Los tratamientos consistieron: 1) Testigo (T; n = 3), los borregos recibieron una dieta a base de maíz-soya-forraje, sin adición de zinc (Zn); 2) ZnMet35, dieta testigo más la adición de 35 ppm de Zn a partir de metionina de Zn (ZnMet) y 3) ZnMet70, dieta testigo más la adición de 70 ppm de Zn. La investigación se llevó a cabo entre los meses de julio de 2009 a abril de 2010. El consumo de dietas adicionadas con 70 ppm de Zn elevó (P < 0.05) el volumen del eyaculado (0.9696 vs. 0.7773 y 0.6864 mL) y la cantidad total de espermatozoides por eyaculado (1955.9 vs. 1463 y 1301.1 millones), mejoró (P < 0.05) el porcentaje de espermatozoides con morfología normal (92.870 vs. 89.045 y 86.818%) y elevó (P = 0.09) la concentración plasmática de testosterona (403.87 vs. 332.12 y 264.59 ng/dL) respecto de los borregos que consumieron dietas adicionadas con 35 ppm de Zn orgánico y los del grupo testigo, respectivamente. Se concluye que el consumo adicional de 70 ppm de Zn a partir de ZnMet eleva el volumen, la cantidad y calidad de espermatozoides del eyaculado, así como la concentración plasmática de testosterona en ovinos de pelo criados en condiciones subtropicales. Palabras claves: zinc, calidad seminal, borregos.ABSTRACT With the objective to evaluate the response of additional intake of different levels of organic zinc on the seminal quality of hair lamb, nine prepubertal lambs were used (between 3.5 to 4.5 months of age) with an average weight of 13 kg and assigned to one of three treatments in an experimental design completely randomized. The treatments consisted on 1) Control (C; n = 3); the lambs received a diet from corn-soybean meal-forage, without addition of organic zinc (Zn); 2) ZnMet35, control diet plus addition of 35 ppm zinc from Zn methionine (ZnMet); and 3) ZnMet70, control diet plus addition of 70 ppm Zn. The investigation was carried out between July 2009 and April 2010. The consumption of the diet supplemented with 70 ppm organic Zn raised the seminal volume (P<0.05), (0.9696 vs. 0.7773 y 0.6864 mL), and the total of spermatozoa per ejaculation (1955.9 vs. 1463 and 1301.1 millions), improved (P<0.05) the percentage of spermatozoa with normal morphology (92.870 vs. 89.045 and 86.818 %), as well as the plasmatic concentration of testosterone (403.87 vs. 332.12 and 264.59 ng/dL; p = 0.09) respect to the treatments that received a diet supplemented with 35 ppm of organic Zn, and the control group, respectively. It is concluded, that consumption of diet supplemented with 70 ppm of Zn from ZnMet, raises the ejaculated volume, quantity, quality of spermatozoa, and raises the plasmatic concentration of testosterone, in hair lambs breeding in the subtropical zo...

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Elevated dietary intake of Zn-methionate is associated with increased sperm DNA fragmentation in the boar

Cited by 28 publications

References 23 publications

Dynamics of sperm DNA fragmentation in raw boar semen and fertility

Dynamics of sperm DNA fragmentation in raw boar semen and fertility

The effect of sperm DNA fragmentation on the dynamics of the embryonic development in intracytoplasmatic sperm injection

Respuesta al consumo adicional de zinc orgánico en la calidad seminal de ovinos de pelo

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