Elevated dosage of Ulp1 disrupts telomeric silencing in Saccharomyces cerevisiae

Abraham, Neethu Maria; Mishra, Krishnaveni

doi:10.1007/s11033-018-4415-1

Cited by 4 publications

(4 citation statements)

References 41 publications

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“…The association of Ulp1 with NPCs is particularly intriguing and has led to the analysis of its role in various processes performed by NPCs, including the regulation of nuclear transport (Stade et al, 2002;Panse et al, 2006;Lewis et al, 2007), certain DNA repair pathways (Zhao et al, 2004;Palancade et al, 2007;Freudenreich and Su, 2016), and the regulation of gene expression Bonnet et al, 2015;Abraham and Mishra, 2018). Among the various mechanisms by which NPCs can influence gene expression is their direct interactions with chromatin.…”

Section: Introductionmentioning

confidence: 99%

Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation

et al. 2020

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In addition to their role in regulating transport across the nuclear envelope, increasing evidence suggests nuclear pore complexes (NPCs) function in regulating gene expression. For example, the induction of certain genes (e.g., yeast INO1) is accompanied by their movement from the nuclear interior to NPCs. As sumoylation has been linked to the regulation of chromatin spatial organization and transcriptional activity, we investigated the role of sumoylation in the expression and NPC recruitment of the INO1 gene. We observed that induction of INO1 is accompanied by both increased and decreased sumoylation of proteins associated with specific regions along the INO1 locus. Furthermore, we show that the E3 ligase Siz2/Nfi1 is required for targeting the INO1 locus to the NPC where it interacts with the SUMO isopeptidase Ulp1. Our data suggest that this interaction is required for both the association of INO1 with the NPC and for its normal expression. These results imply that sumoylation is a key regulator of INO1 targeting to the NPC, and a cycle of sumoylation and NPC-associated desumoylation events contribute to the regulation of INO1 expression.

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Section: Introductionmentioning

confidence: 99%

Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation

et al. 2020

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“…ChIP was performed as described [ 95 ]. Briefly, yeast cells were grown to OD 600 0.8–1 and crosslinked for 10 mins with formaldehyde (final conc.…”

Section: Methodsmentioning

confidence: 99%

Transcription termination complex, Rtt103-Rai1-Rat1, regulates sub-telomeric transcripts in Saccharomyces cerevisiae

Ramalingam

Mishra

2023

RNA Biology

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Telomeres are terminal structures that define the ends of linear chromosomes. They harbour specialized ribonucleoprotein complexes which play a major role in genome integrity by preventing unscheduled DNA damage repair events. Genes located adjacent to telomere repeat sequences are repressed by a phenomenon called telomere position effect (TPE) via epigenetic silencing. RNA surveillance pathways post-transcriptionally regulate any leaky transcripts arising from the telomeres. Recently, multiple non-coding RNA species originate from telomere ends, namely, TERRA (telomeric repeat‐containing RNA), ARRET, sub-telomeric XUTs and sub-telomeric CUTs have been identified. In this study, we report a role for the transcription termination complex (Rtt103-Rai1-Rat1) in regulating the abundance of the sub-telomeric transcripts in a transcription-dependent manner. We show that the Rtt103 mutants have elevated levels of TERRA and other sub-telomeric transcripts that are usually silenced. Our study suggests that Rtt103 potentially recruits the exonuclease, Rat1 in a RNA polymerase II dependent manner to degrade these transcripts and regulate their levels in the cell.

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“…The copyright holder for this preprint this version posted April 1, 2023. ; https://doi.org/10.1101/2023.03.31.535026 doi: bioRxiv preprint used are mentioned in Table3, Supplementary file 1. ∆∆Ct method was used to calculate fold change (Abraham and Mishra, 2018).…”

Section: Qrt Pcrmentioning

confidence: 99%

Uip4 governs growth phase dependent organelle remodeling by modulatingSaccharomyces cerevisiaelipidome

Deolal

Ramalingam

Das

et al. 2023

Preprint

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When yeast cells are exposed to nutrient-limiting conditions, they undergo transcriptional and translational reprogramming that results in the remodeling of metabolite utilization and organelle architecture. Organelle membranes and contacts also undergo structural and functional alterations. In the budding yeast Saccharomyces cerevisiae, regulated expression of Uip4 is shown to be a critical effector of nuclear shape and function, particularly during the stationary phase. In this work, we demonstrate that the absence of UIP4 affects the morphology of multiple other organelles including mitochondria, endoplasmic reticulum, vacuole and the distribution of lipid droplets. The results show that modulating carbon source, nitrogen availability and cellular energy state impact Uip4 expression. This expression of Uip4 is controlled by the transcription factor Msn2, downstream of Sch9 signaling pathway. Cells lacking Uip4 have poor survival in the stationary phase of the growth cycle. These cellular changes are concomitant with dysregulation of the global lipidome profile and aberrant organelle interaction. We propose that the dynamic and regulated expression of Uip4 is required to maintain lipid homeostasis and organelle architecture which is ultimately required to survive in nutrient-limiting conditions such as stationary phase.

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Elevated dosage of Ulp1 disrupts telomeric silencing in Saccharomyces cerevisiae

Cited by 4 publications

References 41 publications

Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation

Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation

Transcription termination complex, Rtt103-Rai1-Rat1, regulates sub-telomeric transcripts in Saccharomyces cerevisiae

Uip4 governs growth phase dependent organelle remodeling by modulatingSaccharomyces cerevisiaelipidome

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