Elevated expression of galectin-3, thioredoxin and thioredoxin interacting protein in preeclampsia

Farladansky-Gershnabel, Sivan; Heusler, Ishai; Biron‐Shental, Tal; Shechter‐Maor, Gil; Amiel, Aliza; Kidron, Debora; Weisz, Avivit; Einbinder, Yael; Cohen-Hagai, Keren; Benchetrit, Sydney; Zitman‐Gal, Tali

doi:10.1016/j.preghy.2021.10.003

Cited by 9 publications

(9 citation statements)

References 39 publications

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“…One reason for the greater elevation of PYCARD in serum from mothers with PET/IUGR may be a parallel activation of a variety of inflammasomes, but this remains to be investigated. Fetal exposure to inflammation may be harmful and our finding of no differences in PYCARD levels in cord blood emphasizes the placenta's ability to protect the fetus, as our lab has reported with other proteins 33–35 …”

Section: Discussionsupporting

confidence: 71%

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Inflammasome activation in preeclampsia and intrauterine growth restriction

Silber

Dekel

Heusler

et al. 2022

American J Rep Immunol

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Problem: Preeclampsia (PE) and intrauterine growth restriction (IUGR) are leading causes of perinatal complications, affecting 8%-10% of all pregnancies. Inflammasomes are suspected to be one of the mechanisms that lead to the process of term and preterm labors. This study evaluated the inflammasome-dependent inflammation processes in placental tissue of women with PE and IUGR. Methods of Study:In this prospective cohort study, 14 women with PE, 15 with placental-related IUGR and 19 with normal pregnancy (NP) were recruited during admission for delivery. Maternal blood was obtained prior to delivery and neonatal cord blood and placental tissue were obtained after delivery.Results: NLRP7 and PYCARD protein expression were higher in placental PE and IUGR samples versus NP samples. Immunostaining revealed that NLRP7 and PYCARD were upregulated in PE and IUGR placental syncytiotrophoblast, stroma and endothelial cells. PYCARD serum levels were significantly higher in women with PE and IUGR. No significant changes were observed in neonatal cord blood.Conclusions: NLRP7 and PYCARD are key inflammatory proteins that are significantly elevated in PE and IUGR. Better understanding their significance may enable them to become markers of prediction or progression of PE and IUGR.

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Section: Discussionsupporting

confidence: 71%

“…Fetal exposure to inflammation may be harmful and our finding of no differences in PYCARD levels in cord blood emphasizes the placenta's ability to protect the fetus, as our lab has reported with other proteins. [33][34][35] Our cohorts had varying degrees of PE, 8/14 had severe features.…”

Section: Discussionmentioning

confidence: 82%

Inflammasome activation in preeclampsia and intrauterine growth restriction

Silber

Dekel

Heusler

et al. 2022

American J Rep Immunol

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“…Maternal blood first contact with the placenta, then the umbilical vein to the fetus. Fetal blood contacts with umbilical artery first and then passes through the placenta to maternal blood (44). Slit-2, CRP, MCP-1, Gal-3, FINS and other indexes in blood complete maternal-fetal blood circulation through placenta transmission (28,(44)(45)(46)(47)(48)(49)(50)(51).…”

Section: Discussionmentioning

confidence: 99%

“…Fetal blood contacts with umbilical artery first and then passes through the placenta to maternal blood (44). Slit-2, CRP, MCP-1, Gal-3, FINS and other indexes in blood complete maternal-fetal blood circulation through placenta transmission (28,(44)(45)(46)(47)(48)(49)(50)(51). Therefore, we measured maternal peripheral blood and neonatal umbilical artery blood to reflect the metabolic concentration of maternal and neonatal.…”

Section: Discussionmentioning

confidence: 99%

The Role of Slit-2 in Gestational Diabetes Mellitus and Its Effect on Pregnancy Outcome

et al. 2022

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BackgroundSlit guidance ligand 2 (Slit-2), as a member of the Slit family, can regulate the inflammatory response and glucose metabolism. The purpose of this study was to explore the expression of Slit-2 in maternal peripheral blood and neonatal cord blood of gestational diabetes mellitus (GDM) patients and its potential importance in disease progression.MethodsThis study included 57 healthy pregnant women and 61 GDM patients. The levels of Slit-2, C-reactive protein (CRP), monocyte chemoattractant protein-1 (MCP-1), C-peptide (C-P), galectin-3(Gal-3), HbA1c, fasting blood glucose (FBG) and fasting insulin (FINS) in maternal peripheral blood and neonatal cord blood were detected by ELISA. Spearman’s rank correlation test was used to assess the association between peripheral Slit-2 and inflammatory indicators, insulin resistance, and pregnancy outcomes. Logistic regression analysis was used to analyze the risk factors of GDM.ResultsSlit-2 levels in maternal peripheral blood and neonatal cord blood of the GDM patients were higher than those of the HC. Slit-2 levels in maternal peripheral blood and neonatal cord blood of the GDM patients were positively correlated with inflammatory factors CRP and MCP-1 levels. The level of Slit-2 in the maternal peripheral blood of the GDM patients was positively correlated with the level of homeostasis model assessment insulin resistance (HOMA-IR) and HbA1c in maternal peripheral blood, but was negatively correlated with the level of homeostasis model assessment –β (HOMA-β). We also found that the Slit-2 level in the maternal peripheral blood of the GDM patients was negatively correlated with neonatal blood glucose, positively correlated with neonatal weight and independent of neonatal total bilirubin.ConclusionOur study suggests that the abnormal increase in Slit-2 in GDM may be related to its pathogenesis, and it was correlated with neonatal blood glucose and weight in patients with GDM, suggesting that Slit-2 may be a potential biomarker of GDM.

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“…Recent studies showed that SLC6A9 [99], FKBP1B [100], S100A12 [101], SLC6A19 [102], TXN (thioredoxin) [103], TLR9 [104], HP (haptoglobin) [105], ARG1 [106], PINK1 [107], B2M [108], C5AR1 [109], MYADM (myeloid associated differentiation marker) [110], CBS (cystathionine beta-synthase) [111], GPX1 [112], SIAH2 [113], PRDX2 [114], RDH8 [115], CYP11B2 [116], RARRES2 [117], NOX1 [118], IL33 [119], OTC (ornithine transcarbamylase) [120], CYP1A1 [121], NFATC4 [122], TSLP (thymic stromal lymphopoietin) [123], WNT4 [124], MGP (matrix Gla protein) [125], FGFBP1 [126], GHR (growth hormone receptor) [127], ERBB3 [128], GFAP (glial fibrillary acidic protein) [129], CCDC40 [130], CACNB2 [131], CD34 [132], NOTCH3 [133], TERT (telomerase reverse transcriptase) [134], GNB3 [135], TP73 [136], RYR2 [137], ENPEP (glutamyl aminopeptidase) [138], SCN7A [139], WNK4 [140], SFRP5 [141], GDF15 [142], CAV1 [143], KCNA5 [144], FOXC1 [145], ASIC1 [146], VASH2 [147], CXCL8 [148], PAPPA2 [149], KCNMA1 [150], LOX (lysyl oxidase) [151], SPARCL1 [152] and CYP3A5 [153] might have the potential to be used as diagnostic biomarkers of hypertension. Previous studies have reported that RHD (Rh blood group D antigen) [154], S100A12 [155], TXN (thioredoxin) [156], TLR9 [157], S100P [158], TAGLN2 [159], S100A9 [160], CA1 [161], HP (haptoglobin) [162], RPL39 [163], F5 [164], PINK1 [165], B2M [166], S100A11 [167], SLC4A1 [168], CBS (cystathionine beta-sy...…”

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Biological Pathways in Bipolar Disorder by Bioinformatics and Next Generation Sequencing Data Analysis

Vastrad¹,

Vastrad²

2022

Preprint

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Bipolar disorder (BD), also known as psychiatric disorder, affects millions of people all over the world. The aim of this investigation was to screen and verify hub genes involved in BD as well as to explore potential molecular mechanisms. The next generation sequencing (NGS) dataset GSE124326 was downloaded from the Gene Expression Omnibus (GEO) database, which contained 480 samples, including 240 BD and 240 normal controls. Differentially expressed genes (DEGs) were filtered and subjected to gene ontology (GO) and pathway enrichment analyses. A protein–protein interaction (PPI) network and module analysis were used to identify hub genes. The miRNA-hub gene regulatory network and TF-hub gene regulatory network were also constructed. Receiver operating characteristic curve (ROC) analysis was used to validate hub genes. In this work, 957 DEGs, including 477 up regulated and 480 down regulated, were obtained from NGS data. The GO and pathway enrichment analyses of the DEGs showed that the up regulated genes were enriched in the neutrophil degranulation, immune system, transport, cytoplasm and enzyme regulator activity, and the down regulated genes were enriched in extracellular matrix organization, diseases of metabolism, multicellular organismal process, cell periphery and metal ion binding. Finally, through analyzing the PPI network, miRNA-hub gene regulatory network and TF-hub gene regulatory network, we screened hub genes UBB, UBE2D1, TUBA1A, RPL11, RPS24, NOTCH3, CAV1, CNBD2, CCNA1 and MYH11 by the Cytoscape software. The current investigation illustrates a characteristic NGS data in BD, which might contribute to the interpretation of the progression of BD and provide novel biomarkers and therapeutic targets for BD.

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Elevated expression of galectin-3, thioredoxin and thioredoxin interacting protein in preeclampsia

Cited by 9 publications

References 39 publications

Inflammasome activation in preeclampsia and intrauterine growth restriction

Inflammasome activation in preeclampsia and intrauterine growth restriction

The Role of Slit-2 in Gestational Diabetes Mellitus and Its Effect on Pregnancy Outcome

Identification of Key Genes and Biological Pathways in Bipolar Disorder by Bioinformatics and Next Generation Sequencing Data Analysis

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