Elevated expression of hyaluronic acid binding protein 1 (HABP1)/P32/C1QBP is a novel indicator for lymph node and peritoneal metastasis of epithelial ovarian cancer patients

Yu, Haifeng; Liu, Qian; Xin, Tao; Liu, Xing; Dong, Guanglu; Jiang, Qi; Lv, Yanju; Song, Xiaowei; Huang, Dayong; Li, Yanju; Shen, Weitao; Teng, Chong; Zhang, Fubin

doi:10.1007/s13277-013-0986-6

Cited by 27 publications

(26 citation statements)

References 39 publications

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“…Zhang et al [27] reported that HABP1 mediating pathway was essential for tumor cell metastasis. Furthermore, HABP1 expression levels were observed to gradually increase from primary lesions to metastatic lymph node and peritoneal dissemination lesions among the same epithelial ovarian cancer patients [22]. The above findings further confirmed that HABP1 may play a role in the development of malignancies including TNBC, perhaps acting as an oncogene.…”

Section: Discussionsupporting

confidence: 66%

“…HABP1 played an important role in HA-mediated cellular event, such as cell adhesion and tumor invasion [7], sperm maturity and motility [11], and cellular signaling [7,[12][13][14][15][16][17]. It is reported that HABP1 is differentially expressed in carcinoma [18] and is of clinical significance in cancers, such as epidermal carcinoma [19], breast cancer [20], melanoma [21], ovarian cancer [22], and endometrial cancer [23]. It is suggested that the expression level of HABP1 has been found higher in breast cancer cells with respect to that in normal breast epithelial cells; however, the clinical significance of HABP1 protein expression in TNBCs remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Elevated expression of HABP1 is a novel prognostic indicator in triple-negative breast cancers

et al. 2015

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Hyaluronan-binding protein 1 (HABP1) has been documented to overexpress in several malignancies. The aim of our study is to investigate the expression of HABP1 protein in triple-negative breast cancer (TNBC) and its clinical significance. Using immunohistochemistry, HABP1 expression was evaluated in 139 TNBC specimens. The association between HABP1 expression with clinicopathological parameters was assessed using chi-square test. The survival status of patients was analyzed using the Kaplan-Meier and log-rank tests. Cox regression was used for the multivariate analysis of prognostic factors. The results showed that HABP1 overexpression correlated with higher histological grade (P = 0.004), advanced TNM stage (P = 0.008), greater tumor size (P = 0.016), metastasis of lymph node (P < 0.001), and recurrence (P = 0.040). Furthermore, it indicated that patients with HABP1 overexpression had significantly poorer overall survival (OS) and disease-free survival (DFS) compared with patients (P < 0.001 for both). Multivariate Cox regression analysis revealed that elevated HABP1 expression was an independent prognostic factor for both OS (P = 0.035) and DFS (P = 0.013). In contrast to the effect of HABP1 in TNBCs with lymph node metastasis, HABP1 overexpression significantly affects prognosis (OS, P = 0.002; DFS, P = 0.003) in TNBCs without lymph node metastasis. In conclusion, HABP1 protein high expression may contribute to the tumor progression and poor prognosis of TNBC, especially in predicting prognosis in TNBCs without lymph node metastasis.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Elevated expression of HABP1 is a novel prognostic indicator in triple-negative breast cancers

et al. 2015

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“…Other studies have implicated p32 as a key regulator of cell proliferation, adhesion, migration and invasion, especially in metabolically active and rapidly growing tissues (Feng et al , 2002; Kim et al , 2011). p32 expression is elevated tumours of the breast, epidermis and ovary (Fogal et al , 2008; Chen et al , 2009; Kim et al , 2011; Yu et al , 2013; Zhang et al , 2013), and increased expression correlates with malignancy (Chen et al , 2009; Yu et al , 2013). Association of p32 with the cell membrane has been shown to promote migration and invasion in vitro (Prakash et al , 2011) and may aid tumour cells in evading the complement system by inhibiting the haemolytic activity of C1q (Rozanov et al , 2002).…”

Section: Introductionmentioning

confidence: 99%

A role for the mitochondrial-associated protein p32 in regulation of trophoblast proliferation

Matos¹,

Horn²,

Beards³

et al. 2014

MHR: Basic Science of Reproductive Medicine

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p32 is a conserved eukaryotic protein which is primarily expressed in the mitochondria and regulates cell proliferation, migration and metabolism in various tissues. In this study, we sought to examine the expression and function of p32 in the human placenta. p32 was highly expressed in the syncytiotrophoblast, the underlying cytotrophoblast (CTB), the vascular endothelium and by a proportion of cells in the villous stroma in first trimester and term placenta. p32 mRNA and protein expression was significantly higher in the first trimester of pregnancy than at term, and expression in the trophoblast was significantly reduced in placentas from women with fetal growth restriction (FGR). Small interfering RNA (siRNA)-mediated knockdown of p32 in term placental explants significantly reduced the number of Ki67-positive CTB, but did not alter CTB apoptosis or necrosis. p32 knockdown increased lactate production, reduced glucose extraction from culture medium and was associated with reduced MitoTracker dye accumulation in trophoblast mitochondria. p32 knockdown was also associated with a significant reduction in expression of the mitochondrial respiratory complexes I and IV. These data suggest that p32 expression is important for CTB proliferation, via a mechanism involving regulation of normal mitochondrial function. As p32 expression is reduced in FGR placentas, this may contribute to some of the observed placental pathology, such as reduced CTB proliferation and mitochondrial dysfunction.

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“…We incubated sections with primary; rabbit monoclonal antiPro-Ex-C-antibody (MCM2 26H6.19), mouse monoclonal anti-Villin-antibody (clone 1D2C3, ab739 , dilution 1:100) at 4°C overnight (abcam, Cambridge, Ma, USa), antiestrogen receptor (clone gF11, dilution 1:50, Novocastra Laboratories, Newcastle upon tyne, United Kingdom) and anti-progesterone receptor (clone 16, dilution 1:200, Novocastra), followed by incubation with secondary antibodies. Sections from small intestine were used as a positive control for villin, and sections from EMC were used as the positive control for ER and PR receptor expression (20,21). For negative controls, the primary antibodies were removed but replaced with phosphate-buffered saline.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Panel of villin, pro-ex-c, estrogen receptor and progesterone receptor expressions could help in differentiation between endocervical and endometrioid adenocarcinoma

Elfeky

Harb²,

Gertallah³

2017

TJPATH

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Objective: Endocervical and endometrioid adenocarcinoma have marked overlapping features and the differentiation between them is important for their accurate management. Villin is an actin-binding protein which has an important role in the maintenance of microvilli in epithelial cells and epithelial cell-specific anti-apoptotic protein processes. Pro-Ex-C is a marker for higher-risk human papilloma virus (HPV) which targets the cell cycle proteins causing their overexpression. the aim of the study was to clarify the diagnostic and predictive role of villin, Pro-Ex-C, estrogen receptor (ER) and progesterone receptor (PR) expression in endocervical and endometrioid adenocarcinoma. Material and Method:We evaluated villin, Pro-Ex-C, ER and PR expressions in 15 cases of endocervical adenocarcinoma and 30 cases of endometrioid adenocarcinoma. We analyzed the diagnostic and predictive role of that panel in both carcinoma subtypes. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were calculated. Results:Positive villin and Pro-Ex-C expressions were positively correlated with the presence and pattern of cervical stromal invasion (p<0.05). ER was positive in all cases of endometrioid adenocarcinoma. PR was detected in most cases of endometrioid adenocarcinoma. the differences of villin, Pro-Ex-C, ER and PR expression in endocervical and endometrioid adenocarcinoma was statistically significant (p<0.05). this methodology for distinguishing endocervical and endometrioid adenocarcinoma had a sensitivity of 100%, a specificity of 100% and a significant prognostic and predictive role.

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Elevated expression of hyaluronic acid binding protein 1 (HABP1)/P32/C1QBP is a novel indicator for lymph node and peritoneal metastasis of epithelial ovarian cancer patients

Cited by 27 publications

References 39 publications

Elevated expression of HABP1 is a novel prognostic indicator in triple-negative breast cancers

Elevated expression of HABP1 is a novel prognostic indicator in triple-negative breast cancers

A role for the mitochondrial-associated protein p32 in regulation of trophoblast proliferation

Panel of villin, pro-ex-c, estrogen receptor and progesterone receptor expressions could help in differentiation between endocervical and endometrioid adenocarcinoma

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