Elevated expression of neuropeptide signaling genes in the eyestalk ganglia and Y-organ of Gecarcinus lateralis individuals that are refractory to molt induction

Pitts, Natalie L.; Schulz, Hanna M.; Oatman, Stephanie R; Mykles, Donald L.

doi:10.1016/j.cbpa.2017.09.011

Cited by 13 publications

(8 citation statements)

References 56 publications

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“…KO identifiers for putative molt inhibiting hormone (MIH) pathway genes were extracted from the KEGG database. The genes catalogued as MIH pathway genes were identified through literature survey 5 , 29 , 45 . KEGG analysis generated annotated YO gene assignments and DEG determinations from mRNA transcript abundance measurements were identified via ANOVA.…”

Section: Resultsmentioning

confidence: 99%

“…Although the regulatory pathway mediating MIH signaling has not been clearly elucidated, previous work hypothesizes that a membrane receptor triggers downstream cyclic nucleotide effectors leading to inhibition of hormone biosynthesis 4 , 5 , 11 , 12 . Searching our database for sequence similarity to known cyclic nucleotide pathway components, we identified orthologous pathway candidates 45 . Most of the putative pathway components decreased in transcript abundance on entry into premolt (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway

Shyamal

Das

Guruacharya

et al. 2018

Sci Rep

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The intermolt crustacean Y-organ (YO) maintains a basal state mediated by pulsatile release of molt inhibiting hormone (MIH), a neuropeptide produced in the eyestalk ganglia, inhibiting YO ecdysteroidogenesis. Reduction of MIH results in YO activation and the animal enters premolt. In the crab, Gecarcinus lateralis, molting was induced by eyestalk ablation (ESA). ESA animals were injected with either rapamycin, an mTOR inhibitor, or DMSO vehicle at Day 0. YOs were harvested at 1, 3, and 7 days post-ESA and processed for high throughput RNA sequencing. ESA-induced increases in mRNA levels of mTOR signaling genes (e.g., mTOR, Rheb, TSC1/2, Raptor, Akt, and S6 kinase) declined following rapamycin treatment. In concert with mTOR inhibition, mRNA levels of ecdysteroid biosynthesis genes (e.g., Nvd, Spo, Sad, Dib, and Phm) were decreased and accompanied by a decrease in hemolymph ecdysteroid titer. By contrast, rapamycin increased the mRNA level of FKBP12, the rapamycin-binding protein, as well as the mRNA levels of genes associated with Wnt and insulin-like growth factor signaling pathways. Many MIH and transforming growth factor-β signaling genes were down regulated in ESA animals. These results indicate that mTOR activity either directly or indirectly controls transcription of genes that drive activation of the YO.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway

Shyamal

Das

Guruacharya

et al. 2018

Sci Rep

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“…To explore whether Mr‐Dsx is under the regulation of eyestalk‐derived neuropeptides, the effect of UEA on the expression of Mr‐Dsx in the above three tissues was further investigated. It is known that upon ablation of the eyestalk, the expression of neuropeptides such as MIH and other CHH family hormones secreted by the XO‐SG in the eyestalk will be repressed, and the inhibitory effects of these neuropeptides on downstream target genes will be released (Hyde, Elizur, & Ventura, ; Pitts, Schulz, Oatman, & Mykles, ). Our experiment revealed that 1 week after removal of the eyestalk, the expression of Mr‐Dsx was significantly induced in the testis and AG (Figure ), suggesting that repression of eyestalk neuropeptides can enhance the transcriptional activity of Mr‐Dsx in these two organs.…”

Section: Discussionmentioning

confidence: 99%

“…Our experiment revealed that 1 week after removal of the eyestalk, the expression of Mr‐Dsx was significantly induced in the testis and AG (Figure ), suggesting that repression of eyestalk neuropeptides can enhance the transcriptional activity of Mr‐Dsx in these two organs. It is speculated that Mr‐Dsx might be a target of CHH hormones present in the XOSG (Pitts et al, ). Given the crucial roles of gonad‐inhibiting hormone (GIH) in orchestrating the development of testis, it is likely that Mr‐Dsx may be negatively regulated by GIH so that suppression of GIH by eyestalk ablation induced the expression of Mr‐Dsx (Chandler, Elizur, & Ventura, ; Graf et al, ).…”

Section: Discussionmentioning

confidence: 99%

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

et al. 2019

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Macrobrachium rosenbergii is an important aquaculture species known as the king of freshwater prawn. Macrobrachium rosenbergii displays remarkable sexual dimorphism, yet our knowledge of the molecular mechanism underpinning sex determination and differentiation of M. rosenbergii is still fragmentary. In this study, through in silico prediction and experimental characterization, we identified a new Dmrt gene (Mr-Dsx) from M. rosenbergii. The cDNA of Mr-Dsx included a 115 bp 5′ untranslated region (UTR), a 2,568 bp open reading frame (ORF) and a 259 bp 3′ UTR. Mr-Dsx was expressed in a wide range of tissues, and showed dynamic expression changes at different moulting stages in the testis, androgenic gland (AG) and eyestalk. In response to unilateral eyestalk ablation (UEA), Mr-Dsx was significantly induced in the testis and AG. Gene knockdown of Mr-Dsx resulted in pronounced suppression of the insulin-like androgenic hormone (IAG) gene. The results suggest that Mr-Dsx is likely to be negatively regulated by the eyestalk neurohormones, and Mr-Dsx may participate in the transcriptional activation of IAG. The study characterized the potential roles of a newly identified Dmrt homologue from M. rosenbergii, and would contribute to a deeper understanding of the molecular regulatory network underlying sex differentiation and moulting of prawns. K E Y W O R D S crustacean, Dmrt, Macrobrachium rosenbergii, moulting, sexual differentiation

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“…Aside from directly manipulating moulting hormones and/or their related organs, changes in physical parameters such as temperature could also affect the production of neuropeptides in the Yorgan (Chung & Webster, 2005;Pitts et al, 2017). As the Y-organ is negatively regulated by MIH and CHH, and CHH is known to increase upon exposure to environmental stress (Rajendiran et al, 2016), therefore, moult inhibition or delay may occur upon stress exposure (Pitts et al, 2017). For example, maximum growth after moulting of C. sapidus was observed when reared at a temperature of 23°C and salinity of 30 ppt (Cadman & Weinstein, 1988).…”

Section: Temperaturementioning

confidence: 99%

Moult induction methods in soft‐shell crab production

Waiho

Ikhwanuddin

Baylon

et al. 2021

Aquaculture Research

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Soft‐shell crabs are gaining attention internationally as a more lucrative option of selling commercially important portunid species due to their ease of consumption, high nutritional values, and unique and excellent taste. This product, however, is only attainable in captivity as crabs are harvested right after moulting when their exoskeletons are still soft. Among the most crucial factor in soft‐shell crab production is the moult induction method. Shorter moult intervals imply more soft‐shell crabs could be produced, increasing productivity and profit for farmers. This review describes the moulting event, soft‐shell crab production process and production systems, and highlights the currently available and potential moult induction methods, including eyestalk ablation, limb autotomy, the use of ecdysteroid, phytoecdysteroid, biogenic amines and methyl farnesoate, the inhibition of moult‐inhibiting hormone (MIH) and the regulation of physical parameters. This review further compares these moult induction methods and their benefits towards soft‐shell crab production.

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Elevated expression of neuropeptide signaling genes in the eyestalk ganglia and Y-organ of Gecarcinus lateralis individuals that are refractory to molt induction

Cited by 13 publications

References 56 publications

Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway

Transcriptomic analysis of crustacean molting gland (Y-organ) regulation via the mTOR signaling pathway

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

Moult induction methods in soft‐shell crab production

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