Elevated GIGYF2 expression suppresses tumor migration and enhances sensitivity to temozolomide in malignant glioma

Yang, Wanchun; Yuan, Qiuyun; Zhang, Shuxin; Zuo, Mingrong; Li, Tengfei; Li, Junhong; Zhou, Xingwang; Li, Mao; Feng, Wentao; Xia, Xiaoqiang; Chen, Mina; Liu, Yan‐Hui

doi:10.1038/s41417-021-00353-1

Cited by 9 publications

(3 citation statements)

References 25 publications

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“…Preliminary measurement of cell migration was performed using an adapted scratch assay in con uent KNS89 cells according to our previous work (Yang et al 2021). The cells were seeded and grown to near con uence in 6-well plates, then underwent serum starving for another 6 hours.…”

Section: Cell Migration and Invasion Assaymentioning

confidence: 99%

Integrated Analysis Identifies MEOX2 as a Malignant Driver and Prognostic Biomarker in Brain Glioma

Sun

Yang

et al. 2022

Preprint

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Purpose: Glioma is a common type of malignant tumor in the brain, of which glioblastoma (GBM) is the most aggressive and lethal form. Despite current progress in the clinical treatment, the prognosis of GBM is still not satisfied, mainly due to the genomic alternations and complicated gene regulatory network. MEOX2 was originally identified as a homeobox transcriptional factor, that was reported to play a role in several types of cancers. However, the expression pattern and biological functions of MEOX2 in glioma are not well elucidated. This study aims to clarify the role of MEOX2 in glioma.Methods: Bioinformatic analysis from TCGA and CGGA databases was used to investigate the correlation of MEOX2 with glioma malignancy and clinical prognosis. The expression of MEOX2 was confirmed from clinical samples by qPCR and western blot. Lentivirus-mediated MEOX2 overexpression and knockdown were employed to investigate the biological functions of MEOX2 in glioma malignant behaviors. RNA-seq and signaling investigations were performed to reveal the molecular mechanisms.Results: Bioinformatic data from public databases and our cohort showed that MEOX2 expression was highly associated with glioma malignancy and could predict the poor prognosis. MEOX2 positively regulates glioma cell proliferation, migration/invasion and chemoresistance to temozolomide. Mechanistically, MEOX2 regulates gene profiles related to PI3K/AKT/mTORC1 pathways and thus promotes glioma malignancy.Conclusion: Our work identifies the novel function of MEOX2 in gliomas, and provides a potential target for glioma prognosis and clinical interventions.

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Section: Cell Migration and Invasion Assaymentioning

confidence: 99%

Integrated Analysis Identifies MEOX2 as a Malignant Driver and Prognostic Biomarker in Brain Glioma

Sun

Yang

et al. 2022

Preprint

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“…While humans encode two GIGYF paralogs (Table 1), most studies have focused on GIGYF2 perhaps in large part due to its association with neurological conditions such as autism spectrum disorder [134][135][136], schizophrenia [137,138] and, most recently, cancer [153]. In contrast, association studies have implicated GIGYF1 in type 2 diabetes [154][155][156].…”

Section: Concluding Remarks and Future Directionsmentioning

confidence: 99%

eIF4E‐homologous protein (4EHP): a multifarious cap‐binding protein

Christie

Igreja

2021

The FEBS Journal

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The cap-binding protein 4EHP/eIF4E2 has been a recent object of interest in the field of post-transcriptional gene regulation and translational control. From ribosome-associated quality control, to RNA decay and microRNA-mediated gene silencing, this member of the eIF4E protein family regulates gene expression through numerous pathways. Low in abundance but ubiquitously expressed, 4EHP interacts with different binding partners to form multiple protein complexes that regulate translation in a variety of biological contexts. Documented functions of 4EHP primarily relate to its role as a translational repressor, but recent findings indicate that it might also participate in the activation of translation in specific settings. In this review, we discuss the known functions, properties and mechanisms that involve 4EHP in the control of gene expression. We also discuss our current understanding of how 4EHP processes are regulated in eukaryotic cells, and the diseases implicated with dysregulation of 4EHPmediated translational control.

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“…Hence, examining immune-related genes that influence the prognosis of glioma might unveil novel targets for immunotherapy targeting gliomas. In order to enhance the prognosis of gliomas and facilitate more personalized treatments, scientists have investigated various biomarkers associated with immune-related gliomas, including PLOD1 10 , GPX8 11 , and GIGYF2 12 . Additional validation of novel biomarkers associated with glioma prognosis is necessary due to variations in species and limited sample sizes.…”

Section: Introductionmentioning

confidence: 99%

Low expression of GALNT6 gene in gliomas promotes immune infiltration and improves tumor prognosis using bioinformatics analysis

Jiang,

Dong,

Huang

et al. 2023

Preprint

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Background The variation in the expression of GALNT6, an enzyme responsible for adding N-acetylgalactosamine, is linked to the growth of cancer cells and has the potential to serve as a novel indicator for the diagnosis and prognosis of tumors. However, its role and mechanisms in gliomas have not been thoroughly investigated. Our study aimed to examine the GALNT6 expression and its correlation with immune infiltration in gliomas, along with its prognostic significance in gliomas. Methods Access to the Cancer Genome Atlas database is available to assess tumor prognostic indicators, we analyzed the survival rate and expected survival time. The Kaplan-Meier method was utilized to create survival curves. Examining the correlation between GALNT6 and longevity through Cox regression and Nomogram forecasting models. Examining the correlation between clinicopathological characteristics and GALNT6 expression through logistic regression analysis. We also examined the mRNA expression of GALNT6 in tumour tissues and paracancerous tissues of glioma patients by RT-qPCR. The effect of interfering with GALNT6 expression in U87 cells was detected using Transwell cell invasion assay and cell scratch assay. Results The findings revealed a strong correlation between decreased GALNT6 expression and the status of isocitrate dehydrogenase (IDH), as well as a significantly improved overall survival rate.The prognosis of glioma may be influenced by factors such as the status of isocitrate dehydrogenase (IDH), codeletion of 1p/19q, age, and GALNT6 levels. The analysis of GO and KEGG pathways revealed the involvement of three signaling pathways in the regulation of the interaction between extracellular vesicles and GALNT6. In contrast, the mRNA level expression of GALNT6 in the paracancerous tissues was significantly lower than that in the tumour tissues, and the degree of cell invasion and migration were significantly reduced after interfering with the expression of GALNT6 in U87 cells. Conclusions Based on our analysis, it was found that reduced GALNT6 expression could potentially impede tumor advancement and enhance favorable prognosis to a certain degree.

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Elevated GIGYF2 expression suppresses tumor migration and enhances sensitivity to temozolomide in malignant glioma

Cited by 9 publications

References 25 publications

Integrated Analysis Identifies MEOX2 as a Malignant Driver and Prognostic Biomarker in Brain Glioma

Integrated Analysis Identifies MEOX2 as a Malignant Driver and Prognostic Biomarker in Brain Glioma

eIF4E‐homologous protein (4EHP): a multifarious cap‐binding protein

Low expression of GALNT6 gene in gliomas promotes immune infiltration and improves tumor prognosis using bioinformatics analysis

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