Elevated level of arsenic negatively influences nifH gene expression of isolated soil bacteria in culture condition as well as soil system

Chakraborty, Arindam; Chowdhury, Atif Aziz; Bhakat, Kiron; Islam, Ekramul

doi:10.1007/s10653-019-00261-2

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…On the other hand, 16S rRNA gene-based diversity analysis revealed an increase in proteobacterial abundance with increased concentration of arsenic (Chakraborty and Islam, 2018). A similar finding was also reported by Chakraborty et al (2019); they showed the decrease in expression of nifH gene and EPS production of arsenic oxidizing free living diazotrophs by members of Bacillus spp., Klebsiella spp., and Burkholderia spp. at higher arsenic concentrations.…”

Section: Summarizing the Genomics Of Major Plant-growth-promoting Attributes Characterized So Far In Diverse Bacteriasupporting

confidence: 80%

“…Nowadays, nitrogen fixing bacteria serve as key players as bioindicators of heavy metal contamination in soil. In this connection, two important studies by Chakraborty and Islam (2018) and Chakraborty et al (2019) reported a decrease in population of nitrogen-fixing diazotrophs at higher concentrations of arsenic while the abundance of proteobacteria was found to be increased. Moreover, they also reported a decrease in EPS production among free living diazotrophs under high arsenic stress.…”

Section: Role Of Nitrogen Fixation Under Heavy Metal Stressmentioning

confidence: 85%

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Molecular Insight Into Key Eco-Physiological Process in Bioremediating and Plant-Growth-Promoting Bacteria

Mandal¹,

Saha²,

Mandal³

2021

Front. Agron.

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Over the past few decades, the massive increase in anthropogenic activity and industrialization processes has increased new pollutants in the environment. The effects of such toxic components (heavy metals, pesticides, etc.) in our ecosystem vary significantly and are of significant public health and economic concern. Because of this, environmental consciousness is increasing amongst consumers and industrialists, and legal constraints on emissions are becoming progressively stricter; for the ultimate aim is to achieve cost-effective emission control. Fortunately, certain taxonomically and phylogenetically diverse microorganisms (e.g., sulfur oxidizing/reducing bacteria) are endowed with the capability to remediate such undesired components from diverse habitats and have diverse plant-growth-promoting abilities (auxin and siderophore production, phosphate solubilization, etc.). However, the quirk of fate for pollutant and plant-growth-promoting microbiome research is that, even with an early start, genetic knowledge on these systems is still considered to be in its infancy due to the unavailability of in-depth functional genomics and population dynamics data from various ecosystems. This knowledge gap can be breached if we have adequate information concerning their genetic make-up, so that we can use them in a targeted manner or with considerable operational flexibility in the agricultural sector. Amended understanding regarding the genetic basis of potential microbes involved in such processes has led to the establishment of novel or advanced bioremediation technologies (such as the detoxification efficiency of heavy metals), which will further our understanding of the genomic/genetic landscape in these potential organisms. Our review aimed to unravel the hidden genomic basis and eco-physiological properties of such potent bacteria and their interaction with plants from various ecosystems.

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Section: Summarizing the Genomics Of Major Plant-growth-promoting Attributes Characterized So Far In Diverse Bacteriasupporting

confidence: 80%

Section: Role Of Nitrogen Fixation Under Heavy Metal Stressmentioning

confidence: 85%

Molecular Insight Into Key Eco-Physiological Process in Bioremediating and Plant-Growth-Promoting Bacteria

Mandal¹,

Saha²,

Mandal³

2021

Front. Agron.

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“…Although, this type of molecular tool is widely used by the basic researches, it presented here efficient results as the differences in the structure of total diazotrophs in soils with castor bean planting and nifH gene had good amplification and representation of bands in the polyacrylamide gel. Moreover, nifH gene is present in all microorganisms fixing nitrogen that containing highly conserved regions among most diazotrophs (CHAKRABORTY et al, 2019). With this, the nifH gene can be widely used in several researches with positive responses, even in environments with opposite temperatures to our research as demonstrated by IZQUIERDO & NUSSLEIN (2006) that have been used successfully nifH sequences to estimate the diazotrophs richness in tropical forest soil in the arctic Tundra region.…”

Section: Resultsmentioning

confidence: 77%

Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils

Fracetto

Barros

et al. 2019

Cienc. Rural

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There are few studies of microbial diversity in castor bean soils in tropical semiarid environmental. Castor bean products have been widely used around the world justifying the commercial importance of ricinoculture in Brazil’ northeastern semiarid. There is no fertilization or maintenance of litter on the soils from the present study, so we hypothesized that the free-living diazotrophs microorganisms drive the nitrogen (N) input into these soils. We evaluated the communities’ structure and diversity of diazotrophs in tropical semiarid soils with i-Castor bean 50 years of cultivation intercropping with maize (CB-50); ii-Only Castor bean 10 years of cultivation (CB-10) and iii-soil under Caatinga vegetation (CAA). Nitrogenase enzyme activity was performed by the acetylene reduction assay (ARA) and the community profile of nifH gene was separated by denaturing gradient gel polyacrylamide electrophoresis (DGGE). Diazotrophs diversity was estimated by the Shannon (H’) and Simpson (D) indexes. Based on the maintenance of soil N stocks and N-microbial biomass over time, the activity of free-living diazotrophs was determinant in the N-input into these soils. The nifH gene was present and its profiles grouped the CB-50 with CAA treatments, so it reinforced the N-fixing by diazotrophs, which presented a greater diversity in the cultivated soils, even after the land use change for the castor bean implementation.

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“…The reduced nitrogen fixation capacity, on the one hand, may have been due to electron–donor competition and the shunting caused by the reduction of As(V). On the other hand, elevated As levels negatively affected the nifH gene expression in free-living nitrogen-fixing bacteria, as revealed by qPCR [ 58 ]. Collectively, As(V) reduction co-occurred with DNRA using Citrobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Arsenic Mobilization and Transformation by Ammonium-Generating Bacteria Isolated from High Arsenic Groundwater in Hetao Plain, China

Jiang

Shen

Shi

et al. 2022

IJERPH

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Arsenic (As) mobilization in groundwater involves biogeochemical cycles of carbon, iron, and sulfur. However, few studies have focused on the role of nitrogen-metabolizing bacteria in As mobilization, as well as in the transformation between inorganic and organic As in groundwater. In this study, the nitrogen and As metabolisms of Citrobacter sp. G-C1 and Paraclostridium sp. G-11, isolated from high As groundwater in Hetao Plain, China, were characterized by culture experiments and genome sequencing. The results showed Citrobacter sp. G-C1 was a dissimilatory nitrate-reducing bacterium. The dissimilatory nitrate reduction to ammonia (DNRA) and As-detoxifying pathways identified in the genome enabled Citrobacter sp. G-C1 to simultaneously reduce As(V) during DNRA. Paraclostridium sp. G-11 was a nitrogen-fixing bacterium and its nitrogen-fixing activity was constrained by As. Nitrogen fixation and the As-detoxifying pathways identified in its genome conferred the capability of As(V) reduction during nitrogen fixation. Under anaerobic conditions, Citrobacter sp. G-C1 was able to demethylate organic As and Paraclostridium sp. G-11 performed As(III) methylation with the arsM gene. Collectively, these results not only evidenced that ammonium-generating bacteria with the ars operon were able to transform As(V) to more mobile As(III) during nitrogen-metabolizing processes, but also involved the transformation between inorganic and organic As in groundwater.

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Elevated level of arsenic negatively influences nifH gene expression of isolated soil bacteria in culture condition as well as soil system

Cited by 8 publications

References 46 publications

Molecular Insight Into Key Eco-Physiological Process in Bioremediating and Plant-Growth-Promoting Bacteria

Molecular Insight Into Key Eco-Physiological Process in Bioremediating and Plant-Growth-Promoting Bacteria

Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils

Arsenic Mobilization and Transformation by Ammonium-Generating Bacteria Isolated from High Arsenic Groundwater in Hetao Plain, China

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